==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=27-NOV-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER LUMINESCENCE 31-MAR-97 1EMC . COMPND 2 MOLECULE: GREEN FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: AEQUOREA VICTORIA; . AUTHOR G.PALM,A.ZDANOV,A.WLODAWER . 897 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 41294.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 653 72.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 48 5.4 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 384 42.8 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 92 10.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 85 9.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 26 2.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 0 0 0 8 0 4 8 8 4 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 4 A G > 0 0 46 0, 0.0 3,-0.7 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 -26.0 12.2 3.9 -17.8 2 5 A E G > + 0 0 94 1,-0.2 3,-1.5 2,-0.2 4,-0.1 0.773 360.0 66.6 -73.2 -31.3 15.9 4.9 -17.3 3 6 A E G 3 S+ 0 0 81 1,-0.3 3,-0.3 2,-0.2 -1,-0.2 0.540 92.6 64.0 -68.6 -5.9 15.3 8.2 -19.1 4 7 A L G < S+ 0 0 74 -3,-0.7 3,-0.3 1,-0.2 -1,-0.3 0.601 97.1 55.1 -91.6 -15.3 13.0 9.2 -16.1 5 8 A F S < S+ 0 0 5 -3,-1.5 29,-0.5 1,-0.2 30,-0.4 0.238 72.0 106.0-104.5 13.2 15.9 9.1 -13.6 6 9 A T S S+ 0 0 89 -3,-0.3 -1,-0.2 -4,-0.1 2,-0.2 0.631 88.0 16.1 -67.4 -18.5 18.3 11.5 -15.3 7 10 A G S S- 0 0 30 -3,-0.3 27,-0.6 25,-0.1 2,-0.5 -0.442 109.2 -46.2-133.1-155.4 17.6 14.3 -12.8 8 11 A V E -A 33 0A 81 -2,-0.2 25,-0.2 25,-0.2 -2,-0.1 -0.757 52.8-164.3 -84.9 130.7 16.1 14.9 -9.4 9 12 A V E -A 32 0A 8 23,-2.1 23,-2.2 -2,-0.5 2,-0.2 -0.933 16.0-128.3-117.4 132.9 12.7 13.2 -8.9 10 13 A P E -A 31 0A 54 0, 0.0 104,-2.3 0, 0.0 2,-0.4 -0.580 24.5-148.7 -77.0 147.5 10.2 14.0 -6.1 11 14 A I E -Ab 30 114A 6 19,-3.1 19,-1.4 -2,-0.2 2,-0.4 -0.934 10.3-162.3-122.9 145.8 9.0 11.1 -4.1 12 15 A L E -Ab 29 115A 57 102,-2.2 104,-2.5 -2,-0.4 2,-0.4 -0.991 11.4-168.2-128.4 122.1 5.6 10.6 -2.4 13 16 A V E +Ab 28 116A 1 15,-2.7 15,-2.6 -2,-0.4 2,-0.4 -0.956 9.6 176.2-124.0 130.0 5.3 7.9 0.3 14 17 A E E +Ab 27 117A 63 102,-2.5 104,-3.8 -2,-0.4 2,-0.4 -0.961 2.6 175.9-132.9 114.2 2.1 6.5 2.0 15 18 A L E -Ab 26 118A 1 11,-1.9 11,-1.8 -2,-0.4 2,-0.4 -0.944 8.8-173.4-121.5 139.9 2.0 3.7 4.5 16 19 A D E +Ab 25 119A 90 102,-1.8 104,-2.3 -2,-0.4 2,-0.3 -0.995 24.9 170.5-129.8 120.9 -1.1 2.4 6.3 17 20 A G E -Ab 24 120A 10 7,-2.1 7,-1.6 -2,-0.4 2,-0.3 -0.840 31.8-161.8-134.3 173.3 -0.4 -0.1 9.0 18 21 A D E -Ab 23 121A 35 102,-1.2 104,-2.9 -2,-0.3 2,-0.5 -0.919 10.3-174.0-153.1 122.7 -1.5 -2.2 11.9 19 22 A V E > S-Ab 22 122A 0 3,-2.6 3,-2.2 -2,-0.3 104,-0.2 -0.956 77.7 -24.6-129.4 112.5 0.8 -3.9 14.5 20 23 A N T 3 S- 0 0 56 102,-2.5 -1,-0.1 -2,-0.5 103,-0.1 0.892 132.1 -44.5 55.0 39.5 -0.8 -6.2 17.0 21 24 A G T 3 S+ 0 0 55 101,-0.3 2,-0.5 1,-0.3 -1,-0.3 0.326 111.7 127.0 89.4 -8.3 -4.0 -4.3 16.5 22 25 A H E < -A 19 0A 79 -3,-2.2 -3,-2.6 29,-0.0 2,-0.4 -0.739 43.7-159.9 -86.1 121.1 -2.4 -0.8 16.6 23 26 A K E +A 18 0A 155 -2,-0.5 2,-0.3 -5,-0.2 -5,-0.2 -0.815 22.2 147.9-106.5 143.6 -3.3 1.3 13.6 24 27 A F E -A 17 0A 2 -7,-1.6 -7,-2.1 -2,-0.4 2,-0.3 -0.945 28.3-139.8-160.5 171.2 -1.3 4.2 12.4 25 28 A S E -A 16 0A 21 -2,-0.3 21,-1.4 -9,-0.2 22,-1.0 -0.989 8.2-165.5-145.2 152.6 -0.3 6.1 9.2 26 29 A V E -AC 15 45A 2 -11,-1.8 -11,-1.9 -2,-0.3 2,-0.4 -0.953 1.9-165.1-137.3 149.0 2.8 7.8 7.9 27 30 A S E -AC 14 44A 47 17,-2.4 17,-2.3 -2,-0.3 2,-0.3 -0.990 10.2-172.6-136.0 143.0 3.4 10.2 5.0 28 31 A G E -AC 13 43A 2 -15,-2.6 -15,-2.7 -2,-0.4 2,-0.3 -0.993 9.6-176.3-141.5 155.3 6.6 11.1 3.4 29 32 A E E +AC 12 42A 150 13,-1.6 13,-1.9 -2,-0.3 2,-0.3 -0.979 28.4 102.1-143.2 153.5 8.1 13.5 0.8 30 33 A G E -AC 11 41A 21 -19,-1.4 -19,-3.1 -2,-0.3 2,-0.3 -0.974 48.6 -95.0 166.4-157.2 11.4 14.0 -0.7 31 34 A E E -AC 10 40A 92 9,-1.9 9,-2.3 -21,-0.3 2,-0.4 -0.961 16.8-148.8-152.1 166.3 13.6 13.5 -3.7 32 35 A G E -AC 9 39A 2 -23,-2.2 -23,-2.1 -2,-0.3 2,-0.6 -0.997 7.6-153.3-144.4 148.1 16.1 11.2 -5.3 33 36 A D E >>> -AC 8 38A 15 5,-2.3 5,-2.2 -2,-0.4 3,-1.8 -0.894 5.5-170.6-125.4 98.8 19.0 11.5 -7.6 34 37 A A G >45S+ 0 0 11 -27,-0.6 3,-0.6 -2,-0.6 35,-0.2 0.806 84.1 69.6 -56.1 -33.2 19.6 8.2 -9.4 35 38 A T G 345S+ 0 0 40 -30,-0.4 -1,-0.3 1,-0.2 -29,-0.1 0.690 115.0 22.8 -57.7 -28.0 22.8 9.6 -10.7 36 39 A Y G <45S- 0 0 7 -3,-1.8 -1,-0.2 30,-0.1 -2,-0.2 0.247 105.3-115.6-124.1 8.7 24.4 9.5 -7.2 37 40 A G T <<5 + 0 0 0 -4,-0.6 182,-2.0 -3,-0.6 2,-0.3 0.838 66.8 155.4 55.9 38.6 22.2 6.8 -5.6 38 41 A K E < -CD 33 218A 35 -5,-2.2 -5,-2.3 180,-0.2 2,-0.4 -0.730 26.4-177.9-108.5 146.8 21.0 9.5 -3.3 39 42 A L E -CD 32 217A 9 178,-1.9 178,-2.0 -2,-0.3 2,-0.4 -0.996 10.9-174.9-143.6 133.5 17.8 9.9 -1.2 40 43 A T E +CD 31 216A 55 -9,-2.3 -9,-1.9 -2,-0.4 2,-0.3 -0.990 21.4 164.0-128.7 119.1 16.4 12.6 1.1 41 44 A L E -CD 30 215A 9 174,-2.1 174,-3.1 -2,-0.4 2,-0.4 -0.999 22.0-166.4-138.1 139.1 13.2 11.8 2.8 42 45 A K E -CD 29 214A 55 -13,-1.9 -13,-1.6 -2,-0.3 2,-0.4 -0.974 14.9-166.8-123.4 135.7 11.2 13.1 5.8 43 46 A F E -CD 28 213A 0 170,-3.0 170,-1.7 -2,-0.4 2,-0.4 -0.950 5.4-165.9-124.4 147.0 8.3 11.2 7.2 44 47 A I E -CD 27 212A 20 -17,-2.3 -17,-2.4 -2,-0.4 2,-0.8 -0.971 23.0-128.7-130.5 147.7 5.6 12.1 9.7 45 48 A C E > -C 26 0A 5 166,-3.0 3,-0.9 -2,-0.4 166,-0.4 -0.869 21.3-168.7 -95.7 111.9 3.2 9.9 11.6 46 49 A T T 3 S+ 0 0 88 -21,-1.4 -20,-0.2 -2,-0.8 -1,-0.1 0.656 78.1 61.9 -79.8 -13.3 -0.3 11.4 10.8 47 50 A T T 3 S- 0 0 71 -22,-1.0 2,-0.6 1,-0.1 -1,-0.2 0.292 115.0-102.2 -95.1 10.6 -2.1 9.4 13.5 48 51 A G S < S+ 0 0 44 -3,-0.9 163,-0.5 -23,-0.2 2,-0.5 -0.919 89.7 20.2 113.1-117.5 -0.3 10.9 16.4 49 52 A K S S- 0 0 188 -2,-0.6 -3,-0.0 161,-0.1 161,-0.0 -0.871 78.7-122.2 -97.9 123.2 2.5 8.8 18.1 50 53 A L - 0 0 9 -2,-0.5 4,-0.1 1,-0.1 -26,-0.1 -0.457 15.2-149.3 -62.3 125.6 4.0 6.0 16.0 51 54 A P S S+ 0 0 31 0, 0.0 -1,-0.1 0, 0.0 83,-0.1 0.734 77.6 55.2 -69.4 -22.6 3.5 2.7 18.1 52 55 A V S S- 0 0 2 1,-0.1 2,-0.2 81,-0.1 80,-0.1 -0.709 100.0 -90.0-111.4 157.1 6.7 1.0 16.7 53 56 A P > - 0 0 10 0, 0.0 3,-1.9 0, 0.0 4,-0.3 -0.470 35.6-127.0 -64.9 138.9 10.3 2.3 16.7 54 57 A W G >> S+ 0 0 14 1,-0.3 3,-2.1 2,-0.2 4,-0.6 0.863 104.6 63.9 -56.9 -39.5 11.1 4.3 13.5 55 58 A P G 34 S+ 0 0 6 0, 0.0 4,-0.3 0, 0.0 -1,-0.3 0.649 92.3 66.7 -60.4 -14.1 14.2 2.3 12.5 56 59 A T G <4 S+ 0 0 3 -3,-1.9 -2,-0.2 1,-0.2 4,-0.1 0.609 102.7 45.5 -81.2 -10.6 11.9 -0.7 12.1 57 60 A L T X> S+ 0 0 0 -3,-2.1 4,-2.8 -4,-0.3 3,-1.2 0.538 81.7 97.8-107.3 -8.7 10.2 0.9 9.1 58 61 A V T 3< S+ 0 0 7 -4,-0.6 -2,-0.1 1,-0.3 -1,-0.1 0.855 85.5 47.6 -47.6 -49.7 13.2 2.2 7.2 59 62 A T T 34 S+ 0 0 27 -4,-0.3 -1,-0.3 2,-0.2 -2,-0.1 0.725 114.8 46.2 -67.3 -22.1 13.3 -0.7 4.8 60 63 A T T <4 0 0 5 -3,-1.2 -2,-0.2 1,-0.1 -1,-0.2 0.890 360.0 360.0 -85.4 -42.4 9.6 -0.5 4.0 61 64 A L < 0 0 15 -4,-2.8 -2,-0.2 -48,-0.1 -3,-0.2 0.863 360.0 360.0 -68.9 360.0 9.5 3.2 3.6 62 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 63 68 A V > 0 0 26 0, 0.0 3,-1.5 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 53.1 12.2 2.9 -3.4 64 69 A Q G > + 0 0 24 1,-0.3 3,-1.0 2,-0.1 16,-0.2 0.387 360.0 87.7 -62.9 2.7 15.2 1.0 -4.8 65 70 A C G 3 S+ 0 0 0 1,-0.2 -1,-0.3 14,-0.2 -31,-0.1 0.436 80.5 67.8 -79.9 -3.2 14.7 2.3 -8.4 66 71 A F G < S+ 0 0 1 -3,-1.5 153,-0.3 -29,-0.1 -1,-0.2 0.186 74.2 121.6 -97.8 12.1 17.0 5.2 -7.1 67 72 A S < - 0 0 0 -3,-1.0 2,-0.6 151,-0.1 153,-0.2 -0.430 69.0-111.4 -75.7 151.5 20.2 3.0 -6.8 68 73 A R B -e 220 0A 71 151,-3.1 153,-3.6 -32,-0.2 -33,-0.1 -0.742 30.0-164.8 -86.5 124.6 23.2 3.8 -8.6 69 74 A Y - 0 0 16 -2,-0.6 5,-0.1 -35,-0.2 153,-0.1 -0.960 20.3-126.0-116.2 116.1 24.0 1.3 -11.3 70 75 A P > - 0 0 34 0, 0.0 3,-1.6 0, 0.0 4,-0.2 -0.228 30.9-103.3 -52.8 153.4 27.6 1.5 -12.7 71 76 A D G > S+ 0 0 88 1,-0.3 3,-1.6 2,-0.2 4,-0.2 0.870 119.7 49.3 -48.0 -53.6 27.8 1.8 -16.4 72 77 A H G 3 S+ 0 0 120 1,-0.3 3,-0.3 2,-0.1 -1,-0.3 0.677 111.6 51.1 -63.9 -18.4 28.8 -1.8 -17.0 73 78 A M G X S+ 0 0 3 -3,-1.6 3,-1.3 1,-0.2 -1,-0.3 0.201 72.7 108.3-105.1 16.7 25.9 -3.1 -14.9 74 79 A K G X + 0 0 76 -3,-1.6 3,-1.5 1,-0.3 -1,-0.2 0.781 67.5 66.5 -70.3 -18.9 23.0 -1.2 -16.4 75 80 A R G 3 S+ 0 0 65 -3,-0.3 -1,-0.3 1,-0.3 115,-0.1 0.297 97.1 60.8 -82.5 13.1 21.4 -4.2 -18.1 76 81 A H G < S+ 0 0 28 -3,-1.3 2,-1.3 115,-0.1 -1,-0.3 0.383 70.2 111.7-123.1 9.9 20.8 -5.5 -14.6 77 82 A D <> + 0 0 2 -3,-1.5 4,-1.0 1,-0.2 112,-0.2 -0.650 32.3 167.3 -88.4 91.2 18.6 -2.7 -13.4 78 83 A F H > + 0 0 1 -2,-1.3 4,-1.3 2,-0.2 3,-0.3 0.955 68.4 62.0 -67.0 -52.9 15.2 -4.3 -13.0 79 84 A F H >4 S+ 0 0 1 1,-0.2 3,-0.7 2,-0.2 4,-0.2 0.873 106.7 42.2 -40.1 -56.9 13.6 -1.4 -11.0 80 85 A K H >4 S+ 0 0 11 1,-0.2 3,-2.0 2,-0.2 -1,-0.2 0.895 107.9 61.4 -63.9 -36.3 14.0 1.3 -13.6 81 86 A S H 3< S+ 0 0 31 -4,-1.0 -1,-0.2 -3,-0.3 -2,-0.2 0.748 98.0 57.4 -62.9 -26.0 12.9 -1.0 -16.5 82 87 A A T - G 0 174A 7 4,-1.6 3,-2.3 -2,-0.6 79,-0.2 -0.776 33.1-109.8 -90.4 116.9 12.4 -10.1 13.8 96 101 A K T 3 S+ 0 0 129 77,-1.9 3,-0.1 -2,-0.7 77,-0.1 -0.222 98.3 0.1 -49.6 124.0 14.4 -12.7 15.6 97 102 A D T 3 S+ 0 0 115 1,-0.3 -1,-0.3 75,-0.1 2,-0.2 0.674 129.5 62.7 67.4 23.5 12.6 -13.8 18.8 98 103 A D S < S- 0 0 29 -3,-2.3 -1,-0.3 1,-0.4 27,-0.2 -0.729 91.4 -55.3-150.5-161.6 9.7 -11.5 18.0 99 104 A G - 0 0 2 -2,-0.2 -4,-1.6 -3,-0.1 -1,-0.4 0.457 45.2-134.3 -69.0-151.8 6.9 -10.8 15.5 100 105 A N E -FH 94 123A 16 23,-1.3 23,-2.0 -6,-0.2 2,-0.4 -0.957 6.0-125.2-167.9 157.0 7.1 -10.1 11.8 101 106 A Y E -FH 93 122A 0 -8,-1.7 -8,-2.2 -2,-0.3 2,-0.5 -0.927 10.0-161.4-114.2 136.3 5.8 -7.7 9.2 102 107 A K E -FH 92 121A 107 19,-2.0 19,-2.3 -2,-0.4 2,-0.3 -0.953 22.9-179.7-112.9 127.4 3.9 -8.6 5.9 103 108 A T E -FH 91 120A 8 -12,-2.1 -12,-1.9 -2,-0.5 2,-0.4 -0.978 17.9-173.7-132.3 147.8 4.0 -5.7 3.5 104 109 A R E +FH 90 119A 129 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