==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=16-NOV-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNE SYSTEM 25-JUL-12 4GAG . COMPND 2 MOLECULE: NEUTRALIZING ANTIBODY AP33 HEAVY CHAIN; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR J.A.POTTER,A.OWSIANKA,A.G.N.ANGUS,N.JEFFERY,D.MATTHEWS,Z.KEC . 439 4 4 4 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19471.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 269 61.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 9 2.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 184 41.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 5 1.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 5 1.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 25 5.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 35 8.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 12 2.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 3 3 9 2 4 7 3 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 3 0 5 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 H E 0 0 197 0, 0.0 26,-0.1 0, 0.0 24,-0.1 0.000 360.0 360.0 360.0 -24.6 1.1 -43.3 27.4 2 2 H V - 0 0 17 24,-0.2 2,-0.4 22,-0.1 24,-0.2 -0.282 360.0-162.9 -49.8 137.1 -1.3 -40.4 28.3 3 3 H Q E -A 25 0A 133 22,-2.7 22,-2.6 24,-0.1 2,-0.3 -0.979 6.1-165.0-129.9 141.5 -1.2 -37.6 25.8 4 4 H L E -A 24 0A 13 -2,-0.4 106,-0.5 104,-0.3 2,-0.4 -0.959 5.3-176.5-123.6 142.6 -2.5 -34.0 26.2 5 5 H Q E -A 23 0A 113 18,-1.3 18,-3.1 -2,-0.3 2,-0.2 -0.952 14.0-146.3-146.3 129.5 -3.1 -31.4 23.6 6 6 H E E +A 22 0A 4 -2,-0.4 2,-0.3 16,-0.2 16,-0.2 -0.585 22.8 172.5 -90.4 146.9 -4.3 -27.8 23.9 7 7 H S E +A 21 0A 54 14,-2.5 14,-3.0 -2,-0.2 105,-0.1 -0.985 18.8 92.7-148.5 158.4 -6.5 -26.1 21.4 8 8 H G S S- 0 0 36 -2,-0.3 12,-0.1 12,-0.2 10,-0.0 -0.788 71.3 -23.4 145.0 179.3 -8.4 -22.8 20.9 9 9 H P - 0 0 81 0, 0.0 3,-0.1 0, 0.0 10,-0.1 -0.091 38.1-153.1 -57.1 146.4 -8.0 -19.3 19.5 10 10 H S S S+ 0 0 22 1,-0.2 105,-2.5 104,-0.1 2,-0.4 0.548 83.0 46.9 -93.2 -9.3 -4.6 -17.8 19.1 11 11 H L E +d 115 0B 37 103,-0.2 2,-0.4 136,-0.1 105,-0.2 -0.999 60.8 176.0-140.4 129.0 -5.9 -14.2 19.4 12 12 H V E -d 116 0B 15 103,-2.4 105,-1.7 -2,-0.4 6,-0.1 -0.984 29.5-121.1-130.1 139.3 -8.3 -12.8 22.0 13 13 H K > - 0 0 106 -2,-0.4 3,-2.1 103,-0.2 72,-0.2 -0.637 41.6 -89.2 -82.5 147.9 -9.3 -9.1 22.3 14 14 H P T 3 S+ 0 0 42 0, 0.0 72,-0.2 0, 0.0 -1,-0.1 -0.187 112.7 21.1 -53.8 143.5 -8.7 -7.2 25.5 15 15 H S T 3 S+ 0 0 92 70,-3.5 69,-0.5 1,-0.3 71,-0.1 0.174 102.6 102.6 78.7 -11.0 -11.5 -7.5 27.9 16 16 H Q S < S- 0 0 102 -3,-2.1 69,-2.3 68,-0.1 2,-0.4 -0.272 79.5 -87.6 -93.0-174.0 -13.0 -10.7 26.5 17 17 H T - 0 0 62 66,-0.2 2,-0.5 67,-0.2 66,-0.2 -0.804 28.7-144.7 -99.6 131.2 -12.7 -14.2 27.7 18 18 H L E - B 0 82A 1 64,-2.9 64,-2.9 -2,-0.4 2,-0.4 -0.829 22.4-174.8 -90.4 130.0 -9.8 -16.6 26.8 19 19 H S E + B 0 81A 47 -2,-0.5 2,-0.3 62,-0.2 62,-0.2 -0.995 7.7 172.7-129.2 132.5 -10.9 -20.2 26.4 20 20 H L E - B 0 80A 3 60,-2.1 60,-2.8 -2,-0.4 2,-0.4 -0.986 16.9-147.1-134.3 154.9 -8.8 -23.2 25.8 21 21 H T E -AB 7 79A 34 -14,-3.0 -14,-2.5 -2,-0.3 2,-0.5 -0.900 1.7-154.8-117.7 146.2 -9.4 -26.9 25.7 22 22 H a E -AB 6 78A 0 56,-3.2 56,-2.0 -2,-0.4 2,-0.5 -0.985 11.5-158.0-115.3 119.6 -7.1 -29.8 26.7 23 23 H S E -AB 5 77A 38 -18,-3.1 -18,-1.3 -2,-0.5 2,-0.4 -0.871 9.5-158.2-100.3 128.0 -7.9 -33.1 24.9 24 24 H V E -A 4 0A 7 52,-2.5 2,-0.3 -2,-0.5 -20,-0.2 -0.889 10.8-177.3-112.6 132.7 -6.6 -36.1 26.8 25 25 H T E +A 3 0A 80 -22,-2.6 -22,-2.7 -2,-0.4 3,-0.0 -0.900 59.0 27.9-127.8 150.2 -5.9 -39.5 25.1 26 26 H G S S+ 0 0 58 -2,-0.3 2,-0.3 -24,-0.2 -24,-0.2 0.504 115.7 38.9 79.3 14.1 -4.7 -42.9 26.6 27 27 H D S S- 0 0 84 -24,-0.1 2,-0.4 -26,-0.1 -24,-0.1 -0.912 85.9-100.0-173.1 165.5 -6.0 -42.5 30.1 28 28 H S > - 0 0 51 -2,-0.3 3,-1.2 1,-0.1 -3,-0.1 -0.816 15.5-135.5-105.9 140.4 -9.1 -41.1 31.8 29 29 H I T 3 S+ 0 0 0 -2,-0.4 5,-0.1 1,-0.3 -1,-0.1 0.396 91.3 86.6 -70.0 2.1 -9.4 -37.7 33.5 30 30 H T T 3 S+ 0 0 71 43,-0.1 2,-0.3 24,-0.1 -1,-0.3 0.266 90.4 50.3 -90.9 16.5 -11.3 -39.3 36.6 31 31 H S S < S+ 0 0 65 -3,-1.2 2,-0.0 21,-0.0 68,-0.0 -0.962 101.8 21.5-135.0 155.8 -7.9 -40.0 38.2 32 32 H G S S- 0 0 15 -2,-0.3 21,-1.5 65,-0.1 2,-0.4 -0.161 88.0 -72.6 87.4-179.4 -5.0 -37.4 38.6 33 33 H Y E -EF 52 98C 2 65,-2.2 65,-2.5 19,-0.2 2,-0.6 -0.981 38.2-149.7-126.1 130.1 -4.9 -33.6 38.7 34 34 H W E -EF 51 97C 0 17,-2.7 17,-2.1 -2,-0.4 63,-0.2 -0.920 25.7-175.2-117.3 111.9 -5.4 -31.7 35.4 35 35 H N E -EF 50 96C 0 61,-3.1 61,-1.6 -2,-0.6 2,-0.4 -0.598 26.4-147.1-113.4 161.5 -3.6 -28.4 35.3 36 36 H W E +EF 49 95C 0 13,-2.2 12,-2.4 59,-0.2 13,-1.6 -0.991 21.2 172.0-124.7 138.7 -2.9 -25.3 33.3 37 37 H I E -EF 47 94C 0 57,-2.5 57,-3.2 -2,-0.4 2,-0.3 -0.933 12.5-157.5-136.7 150.5 0.4 -23.5 33.3 38 38 H R E -EF 46 93C 9 8,-2.2 8,-3.0 -2,-0.3 2,-0.5 -0.975 8.8-148.0-136.4 157.4 1.5 -20.6 31.1 39 39 H K E -EF 45 92C 23 53,-2.5 53,-2.4 -2,-0.3 6,-0.2 -0.986 20.8-138.8-125.3 118.6 4.9 -19.2 30.0 40 40 H F > - 0 0 47 4,-2.2 3,-2.7 -2,-0.5 51,-0.2 -0.222 30.4-102.8 -73.7 164.7 5.1 -15.4 29.5 41 41 H P T 3 S+ 0 0 100 0, 0.0 -1,-0.1 0, 0.0 50,-0.1 0.889 126.1 59.9 -60.9 -30.5 7.1 -13.9 26.5 42 42 H G T 3 S- 0 0 58 1,-0.1 -2,-0.0 2,-0.1 -3,-0.0 0.478 121.1-114.2 -73.4 -3.7 9.8 -13.1 29.0 43 43 H N < + 0 0 90 -3,-2.7 -1,-0.1 1,-0.3 2,-0.0 0.227 67.2 141.9 89.2 -6.5 10.0 -16.8 29.7 44 44 H K - 0 0 112 -5,-0.1 -4,-2.2 1,-0.1 2,-0.4 -0.375 44.4-137.1 -62.9 139.7 8.7 -16.8 33.3 45 45 H L E -E 39 0C 13 272,-0.4 271,-0.4 -6,-0.2 2,-0.4 -0.863 18.9-169.9-100.2 138.0 6.4 -19.8 34.1 46 46 H E E -E 38 0C 38 -8,-3.0 -8,-2.2 -2,-0.4 2,-0.3 -0.987 19.3-133.1-127.4 130.0 3.3 -19.2 36.1 47 47 H Y E -E 37 0C 1 267,-0.4 -10,-0.2 -2,-0.4 269,-0.0 -0.658 22.6-178.9 -76.9 132.3 1.0 -22.0 37.6 48 48 H M E - 0 0 0 -12,-2.4 12,-2.8 -2,-0.3 2,-0.3 0.875 41.6 -88.2 -97.9 -50.8 -2.7 -21.3 36.9 49 49 H G E -EG 36 59C 0 -13,-1.6 -13,-2.2 10,-0.2 2,-0.3 -0.950 29.6-112.4 167.9-179.9 -4.6 -24.1 38.5 50 50 H Y E -EG 35 58C 1 8,-2.4 8,-1.8 -2,-0.3 2,-0.4 -0.942 4.2-148.3-136.7 164.5 -6.2 -27.6 38.4 51 51 H I E -EG 34 57C 11 -17,-2.1 -17,-2.7 -2,-0.3 6,-0.2 -0.993 34.8-143.0-121.3 135.3 -9.4 -29.5 38.4 52 52 H S E > -E 33 0C 0 4,-3.2 3,-1.7 -2,-0.4 -19,-0.2 -0.387 29.2 -91.2 -91.8 178.0 -8.9 -32.8 40.1 53 53 H Y T 3 S+ 0 0 80 -21,-1.5 -20,-0.1 1,-0.3 -1,-0.1 0.667 126.8 49.6 -55.7 -21.4 -10.3 -36.3 39.5 54 54 H S T 3 S- 0 0 74 2,-0.1 -1,-0.3 0, 0.0 3,-0.1 0.515 120.9-102.6 -95.9 -12.2 -13.2 -35.5 41.8 55 55 H G S < S+ 0 0 32 -3,-1.7 -2,-0.1 1,-0.4 16,-0.1 0.264 78.8 134.0 102.8 -11.0 -14.2 -32.1 40.2 56 56 H S - 0 0 51 -5,-0.1 -4,-3.2 1,-0.0 -1,-0.4 -0.360 40.7-151.5 -67.5 153.3 -12.5 -30.0 43.0 57 57 H T E -G 51 0C 51 -6,-0.2 2,-0.4 -3,-0.1 379,-0.3 -0.882 17.5-163.8-130.0 161.6 -10.3 -27.1 41.8 58 58 H Y E -G 50 0C 8 -8,-1.8 -8,-2.4 -2,-0.3 2,-0.4 -0.970 15.4-171.7-142.0 127.6 -7.3 -25.1 43.0 59 59 H Y E -G 49 0C 36 -2,-0.4 -10,-0.2 -10,-0.2 2,-0.2 -0.951 31.7-106.3-123.4 144.4 -6.5 -21.7 41.5 60 60 H N >> - 0 0 11 -12,-2.8 3,-2.3 -2,-0.4 4,-1.3 -0.454 30.8-130.3 -58.9 131.0 -3.5 -19.4 41.8 61 61 H L T 34 S+ 0 0 83 1,-0.3 4,-0.4 2,-0.2 -1,-0.2 0.771 106.3 67.2 -58.6 -28.2 -4.6 -16.5 44.0 62 62 H S T 34 S+ 0 0 60 1,-0.2 -1,-0.3 2,-0.1 -2,-0.1 0.748 119.6 22.1 -63.0 -24.1 -3.1 -14.1 41.4 63 63 H L T X> S+ 0 0 1 -3,-2.3 3,-2.5 -15,-0.2 4,-1.4 0.462 87.8 110.5-114.9 -11.5 -5.9 -15.4 39.1 64 64 H R T 3< S+ 0 0 175 -4,-1.3 -3,-0.1 1,-0.3 -2,-0.1 0.760 80.2 53.1 -46.8 -31.6 -8.6 -16.8 41.4 65 65 H S T 34 S+ 0 0 110 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.778 118.9 31.6 -74.9 -25.9 -11.1 -14.0 40.6 66 66 H R T <4 S+ 0 0 53 -3,-2.5 17,-3.0 17,-0.1 2,-0.3 0.490 102.6 84.8-110.3 -4.9 -10.9 -14.4 36.7 67 67 H I E < +C 82 0A 12 -4,-1.4 2,-0.4 15,-0.2 15,-0.2 -0.774 41.6 178.3-109.8 148.8 -10.2 -18.1 36.1 68 68 H S E -C 81 0A 35 13,-2.7 13,-2.2 -2,-0.3 2,-0.5 -0.994 7.0-168.1-138.6 135.4 -12.4 -21.2 35.8 69 69 H I E +C 80 0A 2 -2,-0.4 2,-0.2 11,-0.2 11,-0.2 -0.994 21.5 162.7-119.1 126.2 -11.2 -24.8 35.1 70 70 H T E -C 79 0A 69 9,-1.6 9,-3.0 -2,-0.5 2,-0.3 -0.792 18.1-148.7-132.2 177.2 -13.9 -27.3 34.2 71 71 H R E -C 78 0A 68 7,-0.3 2,-0.4 -2,-0.2 7,-0.2 -0.948 16.1-151.9-145.9 164.0 -14.1 -30.7 32.6 72 72 H D E >>> -C 77 0A 48 5,-2.9 5,-1.8 -2,-0.3 3,-1.1 -0.866 9.6-171.7-136.2 101.8 -16.3 -33.1 30.5 73 73 H T T 345S+ 0 0 72 -2,-0.4 -44,-0.1 1,-0.3 -43,-0.1 0.666 84.5 64.6 -70.5 -13.1 -15.5 -36.7 31.3 74 74 H S T 345S+ 0 0 104 1,-0.2 -1,-0.3 3,-0.1 -2,-0.0 0.753 114.6 31.9 -80.6 -19.9 -17.7 -37.9 28.4 75 75 H K T <45S- 0 0 124 -3,-1.1 -2,-0.2 2,-0.2 -1,-0.2 0.382 105.1-128.0-104.9 -5.4 -15.2 -36.1 26.0 76 76 H N T <5 + 0 0 30 -4,-0.6 -52,-2.5 1,-0.2 2,-0.3 0.944 69.5 123.3 48.7 48.9 -12.1 -36.7 28.2 77 77 H Q E < -BC 23 72A 46 -5,-1.8 -5,-2.9 -54,-0.2 2,-0.3 -0.954 45.5-162.6-128.8 155.3 -11.4 -33.0 27.9 78 78 H Y E -BC 22 71A 0 -56,-2.0 -56,-3.2 -2,-0.3 2,-0.3 -0.933 15.2-145.3-134.7 167.3 -10.9 -30.2 30.4 79 79 H Y E -BC 21 70A 42 -9,-3.0 -9,-1.6 -2,-0.3 2,-0.4 -0.928 10.5-150.1-138.3 146.1 -11.1 -26.5 29.8 80 80 H L E -BC 20 69A 1 -60,-2.8 -60,-2.1 -2,-0.3 2,-0.4 -0.951 12.4-177.6-111.4 137.9 -9.6 -23.2 30.9 81 81 H Q E -BC 19 68A 85 -13,-2.2 -13,-2.7 -2,-0.4 2,-0.5 -0.991 1.4-175.6-131.2 131.5 -11.5 -19.9 30.8 82 82 H L E -BC 18 67A 0 -64,-2.9 -64,-2.9 -2,-0.4 3,-0.3 -0.968 17.0-149.8-125.9 110.3 -9.8 -16.7 31.8 83 82AH N + 0 0 63 -17,-3.0 -66,-0.2 -2,-0.5 -67,-0.1 -0.347 65.6 13.8 -79.8 163.1 -12.1 -13.6 31.9 84 82BH S S S- 0 0 74 -69,-0.5 -1,-0.2 1,-0.1 -67,-0.2 0.876 82.2-165.7 53.5 47.0 -11.3 -9.9 31.3 85 82CH V - 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