==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=26-JUL-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 19-AUG-12 4GOB . COMPND 2 MOLECULE: KAEDE-TYPE FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT; . AUTHOR H.KIM,T.J.GRUNKEMEYER,L.CHEN,R.FROMME,R.M.WACHTER . 865 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33988.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 639 73.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 6.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 408 47.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 69 8.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 66 7.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 30 3.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 4 0 8 0 4 4 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A S 0 0 146 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 -50.0 -5.3 -46.2 -0.2 2 3 A V + 0 0 48 78,-0.1 2,-0.7 75,-0.1 75,-0.0 0.725 360.0 77.1 -70.6 -20.4 -4.1 -45.3 -3.7 3 4 A I S S- 0 0 6 77,-0.1 2,-0.1 74,-0.1 -1,-0.1 -0.834 73.6-166.7 -96.7 113.2 -1.3 -43.3 -2.0 4 5 A K - 0 0 101 -2,-0.7 29,-0.2 2,-0.2 -2,-0.1 -0.413 37.6 -97.2 -94.2 170.6 1.4 -45.6 -0.8 5 6 A S S S+ 0 0 82 1,-0.1 2,-0.4 26,-0.1 29,-0.2 0.789 115.0 37.0 -53.4 -36.4 4.3 -45.0 1.6 6 7 A D S S+ 0 0 99 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.993 74.1 173.7-123.4 129.1 6.7 -44.6 -1.4 7 8 A M E -A 30 0A 3 23,-2.3 23,-2.4 -2,-0.4 2,-0.2 -0.955 23.8-130.6-132.0 152.8 5.5 -42.8 -4.6 8 9 A K E -Ab 29 110A 77 101,-3.2 103,-2.6 -2,-0.3 2,-0.4 -0.600 19.4-151.6 -97.5 162.2 7.3 -41.6 -7.8 9 10 A I E -Ab 28 111A 2 19,-2.6 19,-2.9 101,-0.2 2,-0.4 -1.000 12.9-178.4-137.2 132.9 7.0 -38.1 -9.3 10 11 A K E -Ab 27 112A 93 101,-2.8 103,-1.4 -2,-0.4 2,-0.3 -0.983 13.2-178.3-127.3 146.6 7.2 -36.9 -12.9 11 12 A L E -Ab 26 113A 10 15,-2.3 15,-2.3 -2,-0.4 2,-0.4 -0.992 19.9-170.2-146.1 145.6 6.9 -33.2 -13.9 12 13 A R E -Ab 25 114A 99 101,-2.4 103,-2.8 -2,-0.3 2,-0.4 -0.970 11.4-171.2-134.8 126.0 6.9 -30.9 -16.9 13 14 A M E -Ab 24 115A 0 11,-3.0 11,-2.3 -2,-0.4 2,-0.4 -0.969 4.3-172.8-114.0 131.2 7.1 -27.1 -16.5 14 15 A E E +Ab 23 116A 82 101,-2.0 103,-2.0 -2,-0.4 2,-0.3 -0.986 28.4 148.5-116.5 129.0 6.6 -24.7 -19.4 15 16 A G E -Ab 22 117A 1 7,-2.6 7,-2.3 -2,-0.4 2,-0.3 -0.936 39.7-144.3-154.4 177.1 7.2 -21.1 -18.6 16 17 A T E -Ab 21 118A 2 101,-1.9 103,-2.8 -2,-0.3 2,-0.5 -0.939 9.2-169.8-150.8 125.6 8.4 -17.6 -19.4 17 18 A V E > S-Ab 20 119A 2 3,-1.9 3,-2.4 -2,-0.3 103,-0.2 -0.984 82.9 -19.3-119.3 121.0 10.2 -15.1 -17.1 18 19 A N T 3 S- 0 0 33 101,-2.3 -1,-0.2 -2,-0.5 102,-0.1 0.877 132.1 -49.9 48.7 39.1 10.5 -11.5 -18.4 19 20 A G T 3 S+ 0 0 39 100,-0.5 2,-0.6 1,-0.2 -1,-0.3 0.302 108.1 127.9 88.1 -10.5 9.7 -12.9 -21.9 20 21 A H E < -A 17 0A 64 -3,-2.4 -3,-1.9 1,-0.0 -1,-0.2 -0.723 50.1-145.4 -87.1 117.9 12.3 -15.7 -21.8 21 22 A K E +A 16 0A 110 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.517 26.3 162.3 -85.2 145.0 10.7 -19.1 -22.6 22 23 A F E -A 15 0A 2 -7,-2.3 -7,-2.6 -2,-0.2 2,-0.4 -0.989 26.6-150.9-156.2 161.1 11.9 -22.4 -21.0 23 24 A V E -AC 14 45A 4 22,-1.9 21,-2.9 -2,-0.3 22,-1.6 -0.995 13.9-174.8-135.4 134.7 10.9 -26.0 -20.4 24 25 A I E -AC 13 43A 0 -11,-2.3 -11,-3.0 -2,-0.4 2,-0.4 -0.992 6.1-160.9-128.9 137.1 12.1 -28.1 -17.4 25 26 A E E -AC 12 42A 86 17,-2.5 17,-2.9 -2,-0.4 2,-0.3 -0.909 9.4-167.5-116.6 145.2 11.4 -31.7 -16.8 26 27 A G E -AC 11 41A 9 -15,-2.3 -15,-2.3 -2,-0.4 2,-0.4 -0.965 12.9-175.5-130.4 152.1 11.6 -33.5 -13.4 27 28 A E E +AC 10 40A 90 13,-2.3 13,-2.8 -2,-0.3 2,-0.2 -0.987 29.0 123.9-141.0 135.8 11.7 -37.0 -12.1 28 29 A G E -AC 9 39A 5 -19,-2.9 -19,-2.6 -2,-0.4 2,-0.3 -0.845 39.7-119.1-161.9-158.0 11.7 -38.0 -8.4 29 30 A E E +AC 8 38A 134 9,-2.2 9,-2.7 -21,-0.3 2,-0.3 -0.965 22.2 176.2-156.0 159.9 10.3 -39.8 -5.5 30 31 A G E -AC 7 37A 8 -23,-2.4 -23,-2.3 -2,-0.3 7,-0.3 -0.962 34.4-115.8-162.3 177.7 8.8 -39.1 -2.1 31 32 A K E >>> - C 0 36A 74 5,-2.6 5,-1.7 -2,-0.3 4,-1.4 -0.918 24.9-167.9-124.6 93.7 7.2 -40.4 1.1 32 33 A P T 345S+ 0 0 0 0, 0.0 34,-0.7 0, 0.0 -1,-0.1 0.857 85.9 47.1 -59.3 -38.7 3.7 -38.8 1.3 33 34 A Y T 345S+ 0 0 104 1,-0.2 -28,-0.1 -28,-0.2 -27,-0.1 0.632 114.5 47.3 -78.9 -14.6 3.0 -39.7 4.9 34 35 A E T <45S- 0 0 113 -3,-1.1 33,-0.3 -29,-0.2 -1,-0.2 0.550 109.5-125.4 -96.1 -14.7 6.4 -38.5 6.1 35 36 A G T <5 + 0 0 0 -4,-1.4 175,-2.3 31,-0.3 2,-0.3 0.836 66.0 124.1 75.6 32.3 6.0 -35.3 4.1 36 37 A T E < +CD 31 209A 24 -5,-1.7 -5,-2.6 173,-0.2 2,-0.3 -0.947 29.3 166.5-126.3 150.3 9.3 -35.6 2.1 37 38 A Q E -CD 30 208A 14 171,-1.7 171,-2.1 -2,-0.3 2,-0.3 -0.981 13.9-163.5-157.6 151.9 10.2 -35.6 -1.6 38 39 A T E -CD 29 207A 42 -9,-2.7 -9,-2.2 -2,-0.3 2,-0.3 -0.988 12.5-177.7-134.3 144.6 13.3 -35.3 -3.8 39 40 A M E -CD 28 206A 6 167,-2.2 167,-3.0 -2,-0.3 2,-0.5 -0.997 22.5-141.4-144.0 143.6 13.5 -34.5 -7.5 40 41 A N E -CD 27 205A 75 -13,-2.8 -13,-2.3 -2,-0.3 2,-0.4 -0.923 26.5-161.7 -98.0 131.5 16.1 -34.2 -10.3 41 42 A L E -CD 26 204A 3 163,-3.1 163,-2.2 -2,-0.5 2,-0.4 -0.942 7.8-168.1-121.7 135.2 15.3 -31.4 -12.7 42 43 A K E -CD 25 203A 91 -17,-2.9 -17,-2.5 -2,-0.4 2,-0.5 -0.983 22.5-128.8-124.8 128.4 16.7 -30.9 -16.2 43 44 A V E +C 24 0A 15 159,-2.0 -19,-0.2 -2,-0.4 3,-0.1 -0.639 28.5 174.6 -70.6 121.1 16.4 -27.8 -18.4 44 45 A K E + 0 0 100 -21,-2.9 2,-0.3 -2,-0.5 -20,-0.2 0.748 65.3 13.5 -98.2 -33.8 15.1 -29.0 -21.8 45 46 A E E S+C 23 0A 120 -22,-1.6 -22,-1.9 1,-0.1 -1,-0.3 -0.992 113.0 36.4-143.2 147.8 14.6 -25.6 -23.5 46 47 A G S S- 0 0 24 -2,-0.3 -1,-0.1 1,-0.3 -24,-0.0 0.526 79.4-168.0 90.1 7.3 15.8 -22.1 -22.8 47 48 A A + 0 0 54 1,-0.1 -1,-0.3 2,-0.1 154,-0.1 -0.452 64.0 45.2 -69.3 146.1 19.2 -22.8 -21.3 48 49 A P S S- 0 0 77 0, 0.0 -1,-0.1 0, 0.0 153,-0.1 0.532 88.3-140.5 -83.1 133.7 21.1 -21.0 -19.9 49 50 A L - 0 0 4 151,-0.5 4,-0.1 1,-0.1 -2,-0.1 -0.417 5.4-153.1 -64.1 128.6 18.3 -19.6 -17.7 50 51 A P + 0 0 44 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.278 69.7 61.8 -88.0 9.2 18.9 -15.8 -17.2 51 52 A F S S- 0 0 4 79,-0.1 2,-0.3 80,-0.0 78,-0.2 -0.870 99.5 -73.2-133.1 164.1 17.1 -15.6 -13.9 52 53 A A > - 0 0 3 76,-1.4 3,-1.9 -2,-0.3 4,-0.2 -0.384 34.0-147.0 -62.1 118.5 17.4 -17.0 -10.3 53 54 A Y T >> S+ 0 0 7 -2,-0.3 3,-2.5 1,-0.3 4,-0.7 0.809 89.6 77.8 -57.9 -32.1 16.4 -20.7 -10.3 54 55 A D H 3> S+ 0 0 11 1,-0.3 4,-0.5 2,-0.2 3,-0.5 0.746 80.9 67.9 -52.7 -24.7 15.1 -20.3 -6.7 55 56 A I H <4 S+ 0 0 0 -3,-1.9 -1,-0.3 1,-0.2 -2,-0.2 0.734 104.1 44.2 -69.4 -14.8 11.9 -18.6 -8.0 56 57 A L H X> S+ 0 0 0 -3,-2.5 3,-1.4 -4,-0.2 4,-1.4 0.656 90.3 84.4 -96.1 -20.0 10.8 -21.9 -9.6 57 58 A T H 3< S+ 0 0 2 -4,-0.7 -2,-0.1 -3,-0.5 3,-0.1 0.844 87.0 50.6 -69.8 -34.3 11.6 -24.3 -6.8 58 59 A T T 3< S+ 0 0 28 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.641 107.4 57.4 -75.9 -9.9 8.3 -24.0 -4.8 59 60 A V T <4 0 0 1 -3,-1.4 -1,-0.2 1,-0.1 -2,-0.2 0.832 360.0 360.0 -81.7 -34.5 6.4 -24.6 -8.0 60 61 A F < 0 0 13 -4,-1.4 -2,-0.2 -3,-0.1 -3,-0.1 0.885 360.0 360.0 -71.2 360.0 8.2 -27.9 -8.5 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 65 A N > 0 0 7 0, 0.0 3,-1.9 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 106.8 2.4 -30.9 -4.3 63 66 A R T 3 + 0 0 10 1,-0.3 14,-0.2 13,-0.0 13,-0.2 0.476 360.0 84.8 -73.6 -3.2 0.9 -30.7 -0.9 64 67 A V T 3 S+ 0 0 0 1,-0.2 2,-1.9 12,-0.2 -1,-0.3 0.863 81.2 69.6 -53.6 -35.9 0.3 -34.5 -0.9 65 68 A F S < S+ 0 0 1 -3,-1.9 2,-0.3 -30,-0.1 -1,-0.2 -0.501 80.0 97.3 -89.7 70.2 3.9 -34.5 0.3 66 69 A A S S- 0 0 0 -2,-1.9 2,-0.7 -34,-0.7 -31,-0.3 -0.944 77.5-117.8-152.0 135.2 3.1 -33.1 3.7 67 70 A K B -e 211 0A 107 143,-2.3 145,-2.5 -33,-0.3 -34,-0.1 -0.703 31.8-164.8 -82.1 115.2 2.6 -35.0 6.9 68 71 A Y - 0 0 29 -2,-0.7 6,-0.1 143,-0.2 145,-0.1 -0.881 12.8-135.3-105.0 124.5 -0.9 -34.5 8.2 69 72 A P > - 0 0 41 0, 0.0 3,-1.7 0, 0.0 144,-0.0 -0.355 30.7-104.8 -70.5 162.2 -1.8 -35.4 11.8 70 73 A K T 3 S+ 0 0 216 1,-0.3 4,-0.1 -2,-0.1 -2,-0.0 0.792 114.8 60.7 -60.5 -31.0 -5.1 -37.3 12.3 71 74 A H T 3 S+ 0 0 129 1,-0.1 -1,-0.3 2,-0.1 -3,-0.0 0.480 95.7 66.7 -82.7 -3.5 -7.0 -34.3 13.7 72 75 A I S < S- 0 0 11 -3,-1.7 -1,-0.1 111,-0.1 140,-0.1 -0.973 89.1-121.0-116.9 120.6 -6.6 -32.2 10.5 73 76 A P - 0 0 35 0, 0.0 2,-1.0 0, 0.0 3,-0.1 -0.300 21.0-136.5 -56.4 134.5 -8.4 -33.4 7.3 74 77 A D > - 0 0 26 1,-0.2 4,-2.1 -6,-0.1 5,-0.1 -0.785 23.4-179.1-100.7 91.6 -5.9 -34.0 4.6 75 78 A Y H > S+ 0 0 18 -2,-1.0 4,-0.6 1,-0.2 -1,-0.2 0.840 77.7 54.0 -59.3 -35.1 -7.6 -32.5 1.5 76 79 A F H >4 S+ 0 0 0 2,-0.2 3,-0.8 1,-0.2 4,-0.3 0.947 110.6 40.8 -73.5 -50.2 -4.7 -33.4 -0.8 77 80 A K H >4 S+ 0 0 19 1,-0.2 3,-1.4 -14,-0.2 -1,-0.2 0.897 113.6 55.5 -67.9 -33.0 -4.4 -37.1 -0.1 78 81 A Q H 3< S+ 0 0 143 -4,-2.1 -1,-0.2 1,-0.2 -2,-0.2 0.648 93.1 72.9 -72.4 -11.2 -8.2 -37.4 -0.1 79 82 A S T - G 0 168A 3 4,-3.0 3,-1.8 -2,-0.7 76,-0.2 -0.482 26.1-115.6 -82.4 154.0 7.9 -10.1 -4.8 93 96 A E T 3 S+ 0 0 92 74,-2.1 75,-0.1 1,-0.3 -1,-0.1 0.657 113.1 53.1 -70.4 -14.9 8.6 -6.9 -2.9 94 97 A D T 3 S- 0 0 45 73,-0.2 -1,-0.3 2,-0.1 3,-0.1 0.201 126.6 -92.2-102.7 14.2 10.3 -5.2 -5.8 95 98 A G S < S+ 0 0 17 -3,-1.8 2,-0.1 1,-0.4 -2,-0.1 0.241 79.0 139.1 98.4 -10.6 7.3 -5.7 -8.2 96 99 A G - 0 0 0 -5,-0.1 -4,-3.0 23,-0.1 2,-0.4 -0.491 33.2-170.9 -58.8 132.8 8.2 -9.0 -9.9 97 100 A I E -FH 91 120A 8 23,-2.3 23,-2.6 -6,-0.2 2,-0.4 -1.000 8.2-172.1-133.1 133.7 5.0 -11.0 -10.1 98 101 A C E -FH 90 119A 0 -8,-2.4 -8,-2.7 -2,-0.4 2,-0.4 -0.949 9.2-162.0-122.6 147.3 4.6 -14.6 -11.1 99 102 A T E +FH 89 118A 1 19,-2.4 19,-2.8 -2,-0.4 2,-0.3 -0.961 18.2 175.3-117.9 147.1 1.5 -16.8 -11.7 100 103 A A E -FH 88 117A 1 -12,-2.1 -12,-2.6 -2,-0.4 2,-0.3 -0.989 12.3-169.2-148.4 154.9 1.8 -20.6 -11.7 101 104 A R E -FH 87 116A 16 15,-2.1 15,-1.9 -2,-0.3 2,-0.4 -0.987 2.6-167.2-142.1 148.7 -0.5 -23.6 -12.0 102 105 A N E -FH 86 115A 0 -16,-2.3 -16,-2.3 -2,-0.3 2,-0.6 -0.984 6.7-161.1-137.5 125.5 -0.1 -27.3 -11.5 103 106 A D E -FH 85 114A 57 11,-2.3 11,-2.2 -2,-0.4 2,-0.5 -0.945 20.6-160.2-102.5 116.4 -2.5 -30.0 -12.6 104 107 A I E +FH 84 113A 0 -20,-2.3 -20,-1.2 -2,-0.6 2,-0.3 -0.877 16.6 167.1-109.1 125.9 -1.7 -33.1 -10.6 105 108 A T E - 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