==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=5-JUL-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE 07-SEP-12 4H04 . COMPND 2 MOLECULE: LACTO-N-BIOSIDASE; . SOURCE 2 ORGANISM_SCIENTIFIC: BIFIDOBACTERIUM BIFIDUM; . AUTHOR T.ITO,T.KATAYAMA,J.WADA,R.SUZUKI,H.ASHIDA,T.WAKAGI,K.YAMAMOT . 1265 2 2 2 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 46897.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 842 66.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 121 9.6 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 134 10.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 2 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 114 9.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 146 11.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 275 21.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 13 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 5 4 5 0 0 0 0 4 0 4 1 5 0 2 0 0 2 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 4 11 1 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 6 8 2 8 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 8 0 0 0 0 0 4 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 30 A S 0 0 85 0, 0.0 1225,-0.2 0, 0.0 1139,-0.0 0.000 360.0 360.0 360.0 166.2 62.9 36.4 56.1 2 31 A S - 0 0 1 1223,-2.6 1138,-2.5 1,-0.1 1139,-0.3 0.741 360.0-138.1 -77.2 -22.9 65.9 35.1 54.0 3 32 A G - 0 0 20 1222,-0.3 1134,-0.1 1,-0.2 -1,-0.1 0.593 34.4-179.5 71.6 13.2 66.9 38.5 52.7 4 33 A L + 0 0 11 1221,-0.2 -1,-0.2 1134,-0.1 1134,-0.2 -0.182 7.5 162.1 -44.9 126.5 67.5 36.9 49.3 5 34 A V E -A 1137 0A 69 1132,-1.7 1132,-2.8 -3,-0.1 -1,-0.0 -0.986 44.3 -91.7-152.3 141.8 68.7 39.5 46.7 6 35 A P E -A 1136 0A 81 0, 0.0 1130,-0.3 0, 0.0 1128,-0.0 -0.221 30.3-135.0 -51.7 140.9 70.4 39.5 43.3 7 36 A R - 0 0 89 1128,-3.3 1129,-0.1 1142,-0.1 1127,-0.0 0.892 27.5-169.6 -67.0 -43.8 74.1 39.7 43.6 8 37 A G - 0 0 70 1127,-0.4 3,-0.1 1,-0.1 1128,-0.1 0.831 7.5-162.4 58.7 44.5 74.3 42.3 40.9 9 38 A S - 0 0 58 1,-0.1 -1,-0.1 5,-0.0 3,-0.1 -0.218 26.3 -99.9 -55.7 143.7 78.2 42.4 40.3 10 39 A H - 0 0 141 1,-0.1 31,-0.2 33,-0.1 -1,-0.1 -0.271 47.1 -96.1 -58.8 152.4 79.7 45.4 38.5 11 40 A M S S+ 0 0 35 33,-0.1 31,-0.2 -3,-0.1 2,-0.2 -0.215 85.9 26.9 -73.7 158.4 80.4 44.9 34.8 12 41 A G E S-H 41 0B 3 29,-3.4 29,-2.8 71,-0.2 2,-0.3 -0.497 104.2 -14.8 91.2-165.4 83.9 43.9 33.7 13 42 A Y E -H 40 0B 71 27,-0.3 2,-0.4 95,-0.3 27,-0.2 -0.570 50.9-171.5 -74.9 144.7 86.6 42.1 35.5 14 43 A S E -H 39 0B 55 25,-3.0 25,-1.9 -2,-0.3 -2,-0.0 -1.000 21.8-149.9-131.2 134.7 86.7 41.5 39.2 15 44 A A S S+ 0 0 66 -2,-0.4 2,-0.5 23,-0.2 23,-0.3 0.688 86.1 75.0 -69.8 -18.2 89.8 40.1 41.1 16 45 A T + 0 0 108 21,-0.1 23,-0.2 23,-0.1 -1,-0.0 -0.871 55.9 143.9 -95.8 122.5 87.3 38.6 43.6 17 46 A A - 0 0 31 -2,-0.5 21,-0.2 21,-0.2 2,-0.0 -0.970 53.6-101.8-147.2 150.3 85.2 35.5 42.5 18 47 A P > - 0 0 29 0, 0.0 3,-1.6 0, 0.0 1147,-0.1 -0.428 35.1-121.7 -73.0 153.5 84.1 32.6 44.6 19 48 A V T 3 S+ 0 0 127 1,-0.3 1146,-0.2 121,-0.0 1147,-0.1 0.763 106.5 39.3 -65.5 -34.9 86.3 29.6 43.9 20 49 A N T 3 S+ 0 0 72 1144,-1.9 -1,-0.3 1145,-0.0 1145,-0.1 -0.010 74.3 147.1-111.4 32.1 83.5 27.3 42.7 21 50 A L < - 0 0 16 -3,-1.6 2,-0.1 1,-0.1 1142,-0.0 -0.431 60.9 -99.1 -54.4 134.2 81.4 29.7 40.7 22 51 A T - 0 0 40 117,-0.0 -1,-0.1 1,-0.0 -2,-0.0 -0.415 24.9-146.0 -61.5 137.5 80.0 27.7 37.8 23 52 A R - 0 0 76 116,-0.1 116,-0.1 -2,-0.1 -1,-0.0 -0.950 33.5-117.9 -96.7 117.1 81.7 27.9 34.4 24 53 A P - 0 0 2 0, 0.0 107,-0.1 0, 0.0 2,-0.1 -0.188 32.4-113.9 -59.5 141.4 78.8 27.6 31.9 25 54 A A - 0 0 12 105,-0.1 2,-0.3 454,-0.1 4,-0.1 -0.371 29.5-161.3 -73.6 161.1 79.1 24.5 29.8 26 55 A T - 0 0 3 -2,-0.1 4,-0.1 105,-0.0 101,-0.1 -0.925 20.3-108.5-130.8 166.1 79.7 24.5 26.0 27 56 A V S S+ 0 0 0 -2,-0.3 2,-0.2 151,-0.2 434,-0.1 -0.970 110.1 35.4-136.3 125.0 79.2 22.0 23.3 28 57 A P S S- 0 0 2 0, 0.0 433,-0.1 0, 0.0 -1,-0.1 0.567 110.4-120.6 -56.4 157.4 81.8 20.9 22.1 29 58 A S - 0 0 9 -2,-0.2 2,-0.8 438,-0.1 -2,-0.1 -0.351 11.3-131.2 -66.9 155.6 83.4 21.0 25.5 30 59 A M - 0 0 2 -4,-0.1 3,-0.5 116,-0.1 2,-0.2 -0.903 32.7-156.8-111.1 97.5 86.5 23.2 25.8 31 60 A D + 0 0 63 -2,-0.8 114,-0.3 1,-0.2 436,-0.0 -0.517 63.9 31.1 -83.0 146.9 88.8 20.7 27.6 32 61 A G S S+ 0 0 70 -2,-0.2 113,-0.2 1,-0.2 -1,-0.2 0.853 70.4 178.9 79.9 25.7 91.9 21.7 29.7 33 62 A W - 0 0 33 -3,-0.5 2,-0.5 111,-0.1 111,-0.2 -0.427 19.7-145.4 -52.4 131.1 90.6 25.1 30.9 34 63 A T E - I 0 143B 88 109,-2.7 109,-2.0 -2,-0.1 2,-0.0 -0.975 18.4-128.0-105.5 126.4 93.2 26.6 33.2 35 64 A D E - I 0 142B 124 -2,-0.5 107,-0.3 107,-0.2 2,-0.2 -0.358 27.3-170.9 -79.8 148.9 91.6 28.6 36.0 36 65 A G E - 0 0 41 105,-2.4 2,-0.4 2,-0.2 105,-0.2 -0.714 32.8 -75.8-122.1-174.2 92.6 32.2 36.7 37 66 A T E S- 0 0 100 -2,-0.2 -21,-0.1 103,-0.1 105,-0.1 -0.667 88.1 -12.8 -88.2 134.0 91.9 34.7 39.4 38 67 A G E S- 0 0 12 -2,-0.4 103,-2.8 -23,-0.3 2,-0.3 -0.380 76.1 -95.8 83.0-160.0 88.6 36.4 39.6 39 68 A A E -HI 14 140B 4 -25,-1.9 -25,-3.0 101,-0.3 2,-0.4 -0.976 17.5-124.8-156.7 166.1 85.8 36.6 37.0 40 69 A W E -HI 13 139B 0 99,-2.9 99,-3.1 -2,-0.3 2,-0.5 -0.947 19.0-157.1-125.4 137.5 84.4 38.8 34.2 41 70 A T E -HI 12 138B 12 -29,-2.8 -29,-3.4 -2,-0.4 2,-0.4 -0.941 13.6-139.0-122.1 128.2 80.9 40.1 33.9 42 71 A L + 0 0 2 95,-2.4 2,-0.3 -2,-0.5 42,-0.1 -0.645 48.9 135.7 -76.0 130.7 79.0 41.2 30.8 43 72 A G > - 0 0 12 -2,-0.4 3,-2.6 40,-0.3 -33,-0.1 -0.892 55.1 -30.0-161.4-170.7 77.0 44.4 31.8 44 73 A E T 3 S+ 0 0 157 1,-0.3 -33,-0.1 -2,-0.3 3,-0.1 -0.224 126.8 22.7 -59.2 134.4 76.0 47.9 30.7 45 74 A G T 3 S+ 0 0 38 27,-0.5 -1,-0.3 1,-0.4 2,-0.2 0.368 85.8 138.9 94.2 -12.3 78.7 49.4 28.6 46 75 A T < - 0 0 1 -3,-2.6 28,-0.8 38,-0.1 -1,-0.4 -0.548 31.6-173.4 -66.6 138.7 80.2 46.0 27.5 47 76 A R E -j 74 0B 71 -2,-0.2 38,-2.6 26,-0.2 2,-0.6 -0.825 30.6-122.1-123.6 165.2 81.2 46.1 23.8 48 77 A V E -jk 75 85B 0 26,-2.3 28,-2.4 -2,-0.3 2,-0.4 -0.952 38.8-166.7-104.3 117.1 82.4 43.7 21.2 49 78 A V E +jk 76 86B 4 36,-3.0 38,-2.6 -2,-0.6 2,-0.3 -0.830 18.8 138.5-109.1 135.1 85.6 45.2 20.0 50 79 A S E - k 0 87B 8 26,-2.3 28,-0.4 -2,-0.4 38,-0.1 -0.980 49.7 -76.6-154.6-179.5 87.5 44.2 16.9 51 80 A S > - 0 0 62 36,-0.5 3,-0.6 -2,-0.3 4,-0.2 -0.134 52.0-103.6 -66.2 167.6 89.6 45.2 13.8 52 81 A D G > S+ 0 0 132 1,-0.2 3,-1.5 2,-0.2 4,-0.3 0.896 122.4 59.3 -60.1 -38.4 87.8 46.5 10.8 53 82 A A G 3 S+ 0 0 95 1,-0.3 3,-0.2 2,-0.1 -1,-0.2 0.771 109.0 43.2 -65.0 -26.8 88.2 43.2 9.1 54 83 A L G <> S+ 0 0 4 -3,-0.6 4,-2.5 1,-0.2 3,-0.3 0.277 82.2 111.2 -96.4 0.9 86.3 41.5 11.9 55 84 A A H <> S+ 0 0 21 -3,-1.5 4,-2.9 1,-0.2 5,-0.2 0.853 74.0 50.7 -43.2 -53.3 83.6 44.1 12.0 56 85 A A H > S+ 0 0 74 -4,-0.3 4,-2.3 -3,-0.2 -1,-0.2 0.892 112.1 47.2 -55.7 -45.9 80.8 42.0 10.6 57 86 A R H > S+ 0 0 23 -3,-0.3 4,-2.4 2,-0.2 -2,-0.2 0.890 114.6 48.6 -61.6 -39.6 81.5 39.1 13.2 58 87 A A H X S+ 0 0 1 -4,-2.5 4,-2.7 1,-0.2 -2,-0.2 0.935 113.0 46.4 -67.6 -41.3 81.7 41.8 16.0 59 88 A Q H X S+ 0 0 111 -4,-2.9 4,-2.3 1,-0.2 5,-0.2 0.878 112.5 48.9 -66.4 -46.3 78.4 43.4 15.0 60 89 A S H X S+ 0 0 72 -4,-2.3 4,-2.2 -5,-0.2 -1,-0.2 0.926 113.8 47.2 -61.6 -42.5 76.6 40.1 14.6 61 90 A L H X S+ 0 0 0 -4,-2.4 4,-3.2 1,-0.2 5,-0.3 0.938 111.2 51.5 -63.9 -42.3 77.8 38.9 18.1 62 91 A A H X S+ 0 0 3 -4,-2.7 4,-2.1 1,-0.2 11,-0.4 0.918 111.7 46.6 -62.2 -41.2 76.9 42.3 19.7 63 92 A S H X S+ 0 0 69 -4,-2.3 4,-1.7 1,-0.2 -1,-0.2 0.910 114.4 48.2 -68.4 -42.5 73.3 42.1 18.3 64 93 A E H X S+ 0 0 12 -4,-2.2 4,-2.2 -5,-0.2 5,-0.2 0.924 112.8 46.2 -61.6 -45.2 73.0 38.5 19.3 65 94 A L H X S+ 0 0 0 -4,-3.2 4,-2.5 1,-0.2 6,-0.4 0.871 106.6 59.0 -68.4 -34.1 74.2 39.1 22.9 66 95 A T H X S+ 0 0 44 -4,-2.1 4,-1.6 -5,-0.3 5,-0.3 0.929 109.3 46.4 -56.1 -45.7 72.0 42.1 23.2 67 96 A K H < S+ 0 0 111 -4,-1.7 -2,-0.2 1,-0.2 -1,-0.2 0.913 117.1 41.1 -64.2 -46.6 69.0 39.8 22.5 68 97 A F H < S+ 0 0 38 -4,-2.2 -1,-0.2 1,-0.2 -2,-0.2 0.793 125.4 32.5 -72.9 -31.9 70.1 37.0 24.9 69 98 A T H < S- 0 0 15 -4,-2.5 -2,-0.2 2,-0.2 -1,-0.2 0.614 91.5-131.7-106.9 -8.8 71.2 39.2 27.8 70 99 A D < + 0 0 148 -4,-1.6 2,-0.4 -5,-0.3 -4,-0.1 0.738 69.9 119.2 63.5 21.1 68.9 42.3 27.6 71 100 A V S S- 0 0 26 -6,-0.4 2,-1.1 -5,-0.3 -1,-0.3 -0.959 76.8-112.1-114.1 141.7 71.9 44.5 27.9 72 101 A D - 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0 0 9 -2,-0.2 2,-0.8 438,-0.1 -2,-0.1 -0.377 10.5-132.4 -68.4 154.4 30.5 17.4 29.3 664 59 B M > - 0 0 9 -4,-0.1 3,-0.6 116,-0.1 2,-0.1 -0.857 30.7-157.0-110.3 99.5 27.0 19.1 29.1 665 60 B D T 3 + 0 0 62 -2,-0.8 114,-0.3 1,-0.2 436,-0.0 -0.363 64.6 31.1 -84.6 149.5 25.1 16.3 27.3 666 61 B G T 3 S+ 0 0 67 1,-0.2 -1,-0.2 112,-0.1 113,-0.2 0.790 70.9 175.3 77.6 22.4 21.9 17.0 25.3 667 62 B W < - 0 0 25 -3,-0.6 2,-0.5 111,-0.1 111,-0.2 -0.355 21.5-143.9 -49.3 137.5 22.7 20.5 24.1 668 63 B T E - K 0 777J 87 109,-2.5 109,-2.2 0, 0.0 -1,-0.0 -0.982 15.4-127.8-112.1 124.1 20.0 21.7 21.8 669 64 B D E - K 0 776J 118 -2,-0.5 107,-0.3 107,-0.2 2,-0.2 -0.343 26.3-172.5 -69.4 142.9 21.2 23.9 18.9 670 65 B G E - 0 0 41 105,-2.4 2,-0.3 2,-0.2 105,-0.3 -0.705 34.0 -77.5-120.4-175.3 19.6 27.2 18.3 671 66 B T E S- 0 0 99 -2,-0.2 -21,-0.1 103,-0.1 105,-0.1 -0.645 87.8 -7.0 -85.2 136.5 20.0 29.7 15.5 672 67 B G E S- 0 0 13 -2,-0.3 103,-2.4 -23,-0.3 2,-0.3 -0.401 77.7 -94.0 90.2-159.8 22.9 32.0 15.3 673 68 B A E -JK 648 774J 4 -25,-1.9 -25,-2.8 101,-0.3 2,-0.4 -0.960 18.4-126.8-156.6 158.9 25.8 32.6 17.8 674 69 B W E -JK 647 773J 0 99,-2.7 99,-3.5 -2,-0.3 2,-0.5 -0.974 21.0-156.9-117.2 138.3 26.7 34.9 20.7 675 70 B T E -JK 646 772J 11 -29,-2.0 -29,-3.5 -2,-0.4 2,-0.4 -0.944 11.9-141.4-121.9 128.9 30.0 36.8 20.9 676 71 B L + 0 0 2 95,-2.4 2,-0.2 -2,-0.5 42,-0.1 -0.671 47.5 131.5 -75.9 130.1 31.8 38.1 23.9 677 72 B G > - 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