==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=4-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER TRANSFERASE 28-SEP-07 2RF4 . COMPND 2 MOLECULE: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA4; . SOURCE 2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE; . AUTHOR S.R.GEIGER,C.D.KUHN,P.CRAMER . 702 11 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 37697.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 451 64.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 20 2.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 186 26.5 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 6 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 6 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 4 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 57 8.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 57 8.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 112 16.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 7 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 0 0 1 1 0 0 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 3 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 7 11 1 3 9 3 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 3 3 0 0 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 22 A K 0 0 244 0, 0.0 2,-0.4 0, 0.0 55,-0.0 0.000 360.0 360.0 360.0 174.7 -55.1 -17.9 -56.4 2 23 A Q + 0 0 110 2,-0.1 2,-0.8 89,-0.0 55,-0.0 -0.906 360.0 172.6-148.5 124.4 -54.6 -21.4 -57.7 3 24 A V - 0 0 46 -2,-0.4 2,-0.2 188,-0.0 188,-0.0 -0.937 69.0-116.6-101.3 124.8 -52.4 -23.7 -59.9 4 25 A T - 0 0 95 -2,-0.8 -2,-0.1 1,-0.1 12,-0.0 -0.516 20.8-120.6 -74.6 139.4 -54.7 -26.4 -58.4 5 26 A N - 0 0 5 -2,-0.2 2,-0.4 84,-0.1 11,-0.1 -0.741 25.0-129.0 -87.5 119.3 -52.8 -28.9 -56.2 6 27 A P - 0 0 57 0, 0.0 9,-0.9 0, 0.0 2,-0.6 -0.549 11.1-145.9 -71.0 121.4 -53.2 -32.4 -57.6 7 28 A I E +A 14 0A 106 -2,-0.4 2,-0.3 7,-0.3 7,-0.3 -0.783 45.2 156.4 -84.0 121.8 -54.4 -34.9 -54.9 8 29 A D E >>> +A 13 0A 83 5,-1.7 3,-1.0 -2,-0.6 4,-0.6 -0.860 12.9 10.6-151.1-178.5 -52.6 -38.1 -55.9 9 30 A E T 345S- 0 0 136 -2,-0.3 3,-0.3 1,-0.2 -2,-0.0 -0.180 101.1 -37.6 59.4-144.8 -51.1 -41.5 -55.9 10 31 A K T 345S+ 0 0 181 1,-0.2 -1,-0.2 2,-0.1 0, 0.0 0.248 128.5 69.1 -97.0 6.9 -51.3 -43.9 -52.9 11 32 A N T <45S- 0 0 40 -3,-1.0 -2,-0.2 2,-0.2 -1,-0.2 0.541 82.5-148.2-103.7 -8.8 -51.0 -41.1 -50.3 12 33 A G T <5 + 0 0 20 -4,-0.6 2,-0.3 -3,-0.3 -3,-0.1 0.621 59.0 126.0 51.4 11.8 -54.4 -39.5 -51.0 13 34 A T E < -A 8 0A 33 -5,-0.6 -5,-1.7 -7,-0.1 2,-1.0 -0.755 66.3-120.2 -99.4 144.7 -52.6 -36.2 -50.0 14 35 A S E -A 7 0A 3 81,-2.8 -7,-0.3 -2,-0.3 -9,-0.1 -0.709 25.9-127.4 -94.8 100.8 -52.6 -33.2 -52.2 15 36 A N + 0 0 43 -2,-1.0 81,-0.0 -9,-0.9 179,-0.0 -0.061 31.1 176.5 -40.1 124.8 -49.1 -32.2 -53.2 16 37 A C + 0 0 3 -11,-0.1 73,-2.1 74,-0.1 2,-0.5 0.484 42.6 100.0-116.9 -3.0 -48.7 -28.5 -52.4 17 38 A I E +B 88 0B 4 176,-0.2 176,-1.5 71,-0.2 2,-0.3 -0.750 47.5 178.4 -90.5 124.9 -45.1 -27.7 -53.2 18 39 A V E -BC 87 192B 1 69,-1.9 69,-3.2 -2,-0.5 2,-0.5 -0.917 26.3-130.8-125.1 149.2 -44.5 -26.0 -56.5 19 40 A R E -BC 86 191B 61 172,-3.8 172,-2.4 -2,-0.3 67,-0.2 -0.878 27.0-171.2 -99.5 128.3 -41.4 -24.7 -58.2 20 41 A V E -B 85 0B 9 65,-3.6 65,-2.7 -2,-0.5 2,-0.1 -0.870 13.4-154.5-131.5 106.6 -41.7 -21.1 -59.5 21 42 A P E +B 84 0B 10 0, 0.0 166,-1.0 0, 0.0 167,-0.7 -0.500 25.4 172.1 -71.0 141.5 -39.2 -19.3 -61.7 22 43 A I E -BD 83 186B 3 61,-3.0 61,-2.8 164,-0.2 2,-0.4 -0.988 26.4-147.6-150.1 150.4 -39.4 -15.5 -61.3 23 44 A A E -BD 82 185B 17 162,-2.8 162,-2.7 -2,-0.3 2,-0.3 -0.976 25.1-168.5-119.0 133.0 -37.4 -12.6 -62.5 24 45 A L E -BD 81 184B 6 57,-2.3 57,-1.8 -2,-0.4 2,-0.8 -0.957 26.5-138.8-133.0 148.7 -37.2 -9.5 -60.2 25 46 A Y E -BD 80 183B 89 158,-1.5 158,-1.2 -2,-0.3 2,-0.3 -0.893 37.3-167.7-101.7 105.4 -36.1 -5.9 -60.2 26 47 A V E -B 79 0B 0 53,-2.4 53,-1.5 -2,-0.8 2,-1.4 -0.743 29.1-124.6-105.8 146.4 -34.4 -5.3 -56.9 27 48 A S E -B 78 0B 40 -2,-0.3 51,-0.2 153,-0.2 50,-0.1 -0.678 36.6-153.3 -84.7 86.8 -33.3 -2.3 -55.1 28 49 A L - 0 0 17 -2,-1.4 5,-0.1 49,-0.6 12,-0.0 -0.327 12.7-121.1 -64.5 142.6 -29.6 -3.2 -54.5 29 50 A A > - 0 0 33 1,-0.1 3,-0.8 7,-0.1 -1,-0.1 -0.518 13.7-125.2 -83.0 155.1 -27.9 -1.6 -51.5 30 51 A P G > S+ 0 0 117 0, 0.0 3,-1.3 0, 0.0 -1,-0.1 0.838 111.1 62.7 -65.5 -33.8 -24.8 0.6 -52.0 31 52 A X G 3 S+ 0 0 124 1,-0.3 -3,-0.0 3,-0.0 8,-0.0 0.735 101.6 52.1 -62.3 -27.4 -22.9 -1.6 -49.6 32 53 A Y G X S+ 0 0 41 -3,-0.8 3,-1.1 4,-0.1 7,-0.3 0.101 76.0 124.1-101.2 18.7 -23.4 -4.6 -52.0 33 54 A L T < S+ 0 0 57 -3,-1.3 43,-0.0 1,-0.2 44,-0.0 -0.429 73.5 31.0 -74.6 159.7 -22.1 -3.1 -55.2 34 55 A E T 3 S+ 0 0 166 -2,-0.1 -1,-0.2 1,-0.1 607,-0.1 0.397 131.4 45.5 71.7 -5.5 -19.2 -5.1 -56.9 35 56 A N <> + 0 0 70 -3,-1.1 4,-0.7 605,-0.1 3,-0.4 -0.235 62.6 153.7-163.4 60.6 -21.0 -8.1 -55.2 36 57 A P H > + 0 0 28 0, 0.0 4,-3.3 0, 0.0 5,-0.2 0.646 66.3 73.4 -66.5 -21.6 -24.9 -7.8 -55.9 37 58 A L H > S+ 0 0 69 1,-0.2 4,-1.2 2,-0.2 -5,-0.0 0.967 101.8 40.5 -60.4 -53.4 -25.3 -11.6 -55.6 38 59 A Q H 4 S+ 0 0 77 -3,-0.4 -1,-0.2 1,-0.2 -6,-0.0 0.734 115.5 57.0 -67.4 -19.6 -24.8 -11.5 -51.8 39 60 A G H >< S+ 0 0 6 -4,-0.7 3,-2.5 -7,-0.3 -2,-0.2 0.959 101.1 51.0 -75.4 -52.6 -26.9 -8.3 -51.9 40 61 A V H >X S+ 0 0 0 -4,-3.3 3,-1.1 1,-0.3 4,-0.8 0.759 100.4 66.3 -56.5 -27.3 -30.0 -9.7 -53.5 41 62 A X H 3X S+ 0 0 18 -4,-1.2 4,-2.0 1,-0.3 -1,-0.3 0.485 91.9 60.5 -76.5 0.0 -30.0 -12.5 -51.0 42 63 A K H <4 S+ 0 0 77 -3,-2.5 -1,-0.3 2,-0.2 -2,-0.1 -0.229 108.7 45.0-113.5 34.9 -30.7 -9.8 -48.3 43 64 A Q H <4 S+ 0 0 39 -3,-1.1 -2,-0.2 -17,-0.0 -3,-0.1 0.173 127.5 30.4-144.3 -25.0 -33.9 -9.3 -50.4 44 65 A H H X>S+ 0 0 2 -4,-0.8 4,-1.4 -5,-0.2 5,-0.9 0.782 107.2 65.2-107.0 -50.1 -34.4 -13.1 -50.7 45 66 A L H >X5S- 0 0 2 -4,-2.0 4,-2.8 2,-0.2 3,-0.9 0.852 118.9 -2.7 -51.2-119.1 -33.1 -14.8 -47.6 46 67 A N H 345S+ 0 0 30 1,-0.3 -1,-0.1 -5,-0.3 5,-0.1 0.938 126.8 72.0 -41.9 -51.2 -34.7 -14.2 -44.2 47 68 A P H 345S+ 0 0 55 0, 0.0 -1,-0.3 0, 0.0 -2,-0.2 0.831 121.2 5.0 -31.1 -59.1 -36.9 -11.7 -46.0 48 69 A L H X<5S+ 0 0 34 -4,-1.4 3,-2.5 -3,-0.9 12,-0.6 0.840 96.7 109.1-103.0 -37.3 -38.8 -14.5 -47.7 49 70 A V B 3< -F 58 0B 32 5,-1.9 5,-1.8 1,-0.1 -1,-0.1 -0.984 16.3-149.7-135.2 146.7 -45.8 -14.2 -49.6 54 75 A N T > 5S+ 0 0 122 -2,-0.3 3,-0.9 3,-0.2 -1,-0.1 0.834 90.3 61.9 -85.5 -38.5 -48.8 -12.9 -51.6 55 76 A K T 3 5S+ 0 0 123 1,-0.3 -1,-0.1 2,-0.1 129,-0.0 0.862 123.4 25.2 -58.3 -34.2 -47.5 -12.4 -55.1 56 77 A V T 3 5S- 0 0 22 2,-0.2 -1,-0.3 -36,-0.0 -2,-0.2 0.164 104.9-136.3-112.2 12.9 -46.8 -16.2 -55.3 57 78 A G T < 5 + 0 0 21 -3,-0.9 2,-0.3 1,-0.1 -3,-0.2 0.785 66.7 69.4 31.4 60.1 -49.4 -16.9 -52.7 58 79 A G E + G 0 82B 112 15,-2.3 15,-2.6 1,-0.1 3,-0.6 -0.942 18.7 174.1-128.5 105.7 -28.6 -15.4 -61.3 68 89 A I T 3 + 0 0 29 -2,-0.5 12,-0.2 13,-0.2 -1,-0.1 0.747 60.8 99.5 -81.2 -23.5 -27.8 -11.7 -61.0 69 90 A L T 3 + 0 0 115 1,-0.3 2,-4.6 -3,-0.1 -1,-0.2 0.578 45.0 111.7 -39.7 -10.0 -26.3 -11.9 -64.4 70 91 A D S < S+ 0 0 69 -3,-0.6 10,-0.6 10,-0.3 -1,-0.3 -0.088 76.3 51.7 -66.4 50.7 -29.6 -10.4 -65.6 71 92 A A + 0 0 58 -2,-4.6 -3,-0.0 8,-0.1 8,-0.0 -0.689 65.4 139.4 178.4 124.7 -27.6 -7.3 -66.3 72 93 A D 0 0 139 -2,-0.2 -3,-0.0 1,-0.0 0, 0.0 -0.543 360.0 360.0 179.5 110.5 -24.4 -7.2 -68.4 73 94 A P 0 0 170 0, 0.0 -1,-0.0 0, 0.0 -2,-0.0 0.964 360.0 360.0 -72.9 360.0 -23.1 -4.7 -71.0 74 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 75 112 A P 0 0 156 0, 0.0 -42,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 177.6 -23.2 4.3 -59.6 76 113 A F - 0 0 169 1,-0.0 -48,-0.2 -43,-0.0 0, 0.0 0.615 360.0-174.9 77.3 24.8 -26.1 1.9 -60.3 77 114 A G + 0 0 47 -50,-0.1 -49,-0.6 -44,-0.0 2,-0.1 0.591 65.6 40.4 -20.0 -46.3 -28.8 1.7 -57.5 78 115 A F E -B 27 0B 93 -51,-0.2 2,-0.3 -53,-0.0 -51,-0.2 -0.464 59.5-176.1-109.9 178.8 -31.0 -1.2 -59.0 79 116 A T E -B 26 0B 15 -53,-1.5 -53,-2.4 -2,-0.1 2,-0.3 -0.942 24.0-121.3-172.2 150.4 -30.7 -4.5 -60.9 80 117 A W E +B 25 0B 104 -10,-0.6 2,-0.3 -2,-0.3 -10,-0.3 -0.729 35.2 169.1 -94.3 142.8 -32.5 -7.4 -62.6 81 118 A C E -B 24 0B 0 -57,-1.8 -57,-2.3 -2,-0.3 2,-0.4 -0.974 22.5-140.0-148.4 161.0 -32.0 -11.0 -61.4 82 119 A H E +BG 23 67B 42 -15,-2.6 -15,-2.3 -2,-0.3 2,-0.3 -0.987 26.1 157.5-130.1 134.1 -33.6 -14.4 -61.9 83 120 A V E -B 22 0B 0 -61,-2.8 -61,-3.0 -2,-0.4 2,-0.5 -0.928 35.2-125.5-145.0 169.2 -34.3 -17.1 -59.4 84 121 A N E -BG 21 64B 21 -20,-1.7 -20,-2.6 -2,-0.3 2,-0.2 -0.978 26.3-160.1-121.0 118.2 -36.5 -20.2 -58.9 85 122 A L E -BG 20 63B 4 -65,-2.7 -65,-3.6 -2,-0.5 2,-0.5 -0.616 10.0-143.1 -94.1 156.1 -38.6 -20.2 -55.7 86 123 A Y E -BG 19 62B 10 -24,-1.9 -25,-1.9 -67,-0.2 -24,-0.9 -0.990 25.3-161.4-116.8 127.0 -40.0 -23.3 -54.1 87 124 A V E -BG 18 60B 0 -69,-3.2 -69,-1.9 -2,-0.5 2,-0.7 -0.934 26.0-148.1-127.9 124.2 -43.4 -22.6 -52.6 88 125 A W E +BG 17 59B 5 -29,-2.1 -29,-3.2 -2,-0.5 -71,-0.2 -0.724 33.3 174.6 -77.4 118.1 -45.7 -24.4 -50.0 89 126 A Q + 0 0 15 -73,-2.1 2,-0.2 -2,-0.7 -31,-0.1 -0.566 6.5 176.8-132.5 66.5 -48.9 -23.4 -51.6 90 127 A P - 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