==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=21-JUN-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER SIGNALING PROTEIN 06-APR-11 3RFG . COMPND 2 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-L . SOURCE 2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE; . AUTHOR L.YATIME,K.L.HEIN,J.NILSSON,P.NISSEN . 576 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 25984.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 357 62.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 236 41.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 16 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 12 2.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 58 10.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 32 5.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 10 8 4 6 14 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 2 6 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 6 A V 0 0 100 0, 0.0 287,-2.1 0, 0.0 2,-0.3 0.000 360.0 360.0 360.0 55.7 29.0 -59.4 22.8 2 7 A L E -A 287 0A 13 285,-0.2 2,-0.4 241,-0.2 285,-0.2 -0.666 360.0-162.4 -86.7 138.1 31.7 -60.8 20.4 3 8 A V E -A 286 0A 63 283,-1.8 283,-1.7 -2,-0.3 2,-0.9 -0.979 27.1-109.2-128.6 137.9 31.3 -64.3 19.0 4 9 A L E +A 285 0A 77 -2,-0.4 281,-0.3 281,-0.2 3,-0.1 -0.409 34.7 177.7 -68.4 101.6 33.8 -66.6 17.4 5 10 A R E - 0 0 60 279,-1.9 2,-0.3 -2,-0.9 -1,-0.2 0.706 53.5 -70.3 -78.9 -20.5 32.9 -66.7 13.7 6 11 A G E -A 284 0A 18 278,-1.1 278,-2.4 42,-0.1 2,-0.5 -0.942 43.9 -83.1 154.8-176.2 35.8 -68.9 12.6 7 12 A T E -A 283 0A 57 -2,-0.3 2,-0.6 276,-0.2 276,-0.2 -0.947 18.6-148.9-134.3 113.6 39.5 -69.3 12.0 8 13 A L E -A 282 0A 5 274,-2.0 274,-2.4 -2,-0.5 2,-0.8 -0.699 20.7-160.6 -73.3 118.9 41.5 -68.4 8.9 9 14 A E E +A 281 0A 146 -2,-0.6 272,-0.2 272,-0.2 271,-0.1 -0.828 43.5 112.9-111.6 96.6 44.3 -71.0 8.9 10 15 A G + 0 0 37 -2,-0.8 -1,-0.1 270,-0.7 271,-0.1 0.738 44.3 75.7-125.7 -54.8 47.2 -69.8 6.7 11 16 A H - 0 0 9 269,-0.5 4,-0.1 2,-0.1 3,-0.1 0.017 51.9-146.0 -76.5 172.3 50.6 -69.0 8.2 12 17 A N S S+ 0 0 144 2,-0.1 2,-0.3 269,-0.0 -1,-0.1 0.075 86.3 33.8-123.3 22.3 53.5 -71.0 9.5 13 18 A G S S- 0 0 9 264,-0.1 21,-0.2 267,-0.0 264,-0.1 -0.931 111.0 -50.0-158.2 170.4 54.5 -68.6 12.2 14 19 A W - 0 0 73 -2,-0.3 19,-1.5 19,-0.2 2,-1.5 -0.130 52.0-119.7 -46.9 134.8 52.7 -66.2 14.6 15 20 A V - 0 0 0 262,-1.5 247,-0.3 266,-0.2 17,-0.3 -0.677 39.5-178.9 -74.4 97.1 50.2 -63.9 12.8 16 21 A T + 0 0 10 -2,-1.5 2,-0.3 15,-0.5 16,-0.2 0.630 55.7 13.9 -80.7 -17.6 52.1 -60.9 14.1 17 22 A S E -D 31 0B 11 14,-1.0 14,-2.4 246,-0.2 2,-0.3 -0.974 56.6-153.3-163.3 147.8 49.8 -58.3 12.5 18 23 A L E -D 30 0B 10 246,-0.5 2,-0.4 -2,-0.3 12,-0.2 -0.900 11.1-170.1-122.0 148.6 46.4 -57.7 10.9 19 24 A A E +D 29 0B 4 10,-2.5 10,-2.5 -2,-0.3 2,-0.3 -0.994 7.1 173.6-139.4 143.5 45.3 -55.1 8.5 20 25 A T E -D 28 0B 34 -2,-0.4 2,-0.3 8,-0.2 8,-0.1 -0.976 16.6-146.1-150.8 138.7 41.9 -54.1 7.1 21 26 A S - 0 0 3 3,-0.6 247,-0.3 6,-0.6 6,-0.2 -0.813 19.6-142.9-112.0 146.8 40.9 -51.2 4.9 22 27 A A S S+ 0 0 75 -2,-0.3 49,-0.2 48,-0.2 -1,-0.1 0.785 104.1 65.3 -66.5 -29.4 37.8 -49.1 4.8 23 28 A G S S+ 0 0 35 47,-1.3 49,-0.2 46,-0.3 48,-0.2 0.992 115.3 19.9 -56.2 -68.1 38.4 -49.2 1.0 24 29 A Q > + 0 0 73 1,-0.2 3,-1.6 3,-0.1 -3,-0.6 -0.856 55.7 174.4-112.0 99.6 37.8 -52.9 0.6 25 30 A P T 3 S+ 0 0 62 0, 0.0 -1,-0.2 0, 0.0 17,-0.2 0.664 82.0 76.6 -65.6 -18.1 35.9 -54.5 3.5 26 31 A N T 3 S+ 0 0 94 -3,-0.1 16,-2.9 15,-0.1 2,-0.3 0.716 94.1 68.7 -57.9 -19.2 36.1 -57.5 1.1 27 32 A L E < - E 0 41B 3 -3,-1.6 -6,-0.6 14,-0.2 2,-0.3 -0.846 50.7-179.1-128.7 141.0 39.7 -57.8 2.3 28 33 A L E -DE 20 40B 0 12,-2.7 12,-4.2 -2,-0.3 2,-0.4 -0.944 13.3-158.4-127.0 151.4 42.0 -58.7 5.2 29 34 A L E -DE 19 39B 2 -10,-2.5 -10,-2.5 -2,-0.3 2,-0.4 -0.979 8.4-173.1-132.7 142.8 45.8 -58.6 5.4 30 35 A S E -DE 18 38B 0 8,-1.4 8,-1.8 -2,-0.4 2,-0.2 -0.994 11.7-158.8-141.0 140.8 48.2 -60.4 7.7 31 36 A A E +DE 17 37B 0 -14,-2.4 -14,-1.0 -2,-0.4 -15,-0.5 -0.673 20.5 179.0-104.8 168.5 51.9 -60.5 8.6 32 37 A S > - 0 0 0 4,-0.5 31,-1.6 -17,-0.3 3,-1.4 -0.993 47.9-101.0-163.2 160.3 53.7 -63.4 10.3 33 38 A R T 3 S+ 0 0 97 -19,-1.5 -19,-0.2 -2,-0.3 29,-0.2 0.090 107.8 84.4 -73.3 25.5 57.0 -64.8 11.6 34 39 A D T 3 S- 0 0 29 2,-0.3 -1,-0.3 -20,-0.2 3,-0.1 0.242 109.8-112.9-109.0 10.8 56.9 -66.8 8.4 35 40 A K S < S+ 0 0 110 -3,-1.4 24,-0.5 1,-0.2 2,-0.3 0.738 83.7 95.4 72.6 23.5 58.4 -63.9 6.4 36 41 A T - 0 0 17 -4,-0.5 -4,-0.5 21,-0.1 -2,-0.3 -0.973 60.9-142.4-143.9 153.4 55.2 -63.4 4.4 37 42 A L E -EF 31 56B 0 19,-1.3 19,-2.1 -2,-0.3 2,-0.4 -0.822 17.6-153.6-106.6 159.5 52.0 -61.4 4.2 38 43 A I E -EF 30 55B 18 -8,-1.8 -8,-1.4 -2,-0.3 2,-0.4 -0.981 6.9-145.9-135.0 122.0 48.7 -62.9 3.1 39 44 A S E -EF 29 54B 4 15,-3.0 14,-2.4 -2,-0.4 15,-1.4 -0.691 21.2-158.2 -77.0 137.0 45.8 -61.1 1.6 40 45 A W E -EF 28 52B 2 -12,-4.2 -12,-2.7 -2,-0.4 2,-0.6 -0.968 11.2-152.0-117.6 134.7 42.5 -62.6 2.6 41 46 A K E -EF 27 51B 72 10,-1.8 10,-1.9 -2,-0.4 2,-0.3 -0.943 19.6-150.2-101.3 121.5 39.2 -62.2 0.8 42 47 A L E - F 0 50B 35 -16,-2.9 2,-0.4 -2,-0.6 8,-0.2 -0.716 14.7-174.4 -91.1 140.7 36.4 -62.5 3.3 43 48 A T - 0 0 74 6,-2.3 -2,-0.0 -2,-0.3 -17,-0.0 -0.999 40.1 -91.5-137.9 134.2 33.1 -63.9 2.2 44 49 A G S S+ 0 0 75 -2,-0.4 2,-0.3 1,-0.0 6,-0.0 -0.173 74.8 128.0 -43.2 129.3 29.9 -64.1 4.2 45 50 A D - 0 0 66 1,-0.1 3,-0.1 -39,-0.0 4,-0.1 -0.973 62.9-141.2-177.4 160.1 30.0 -67.5 5.9 46 51 A D S S+ 0 0 132 1,-0.4 3,-0.5 -2,-0.3 2,-0.2 0.810 101.9 28.5 -88.8 -75.1 29.9 -69.9 8.8 47 52 A Q S S+ 0 0 158 1,-0.2 -1,-0.4 2,-0.1 -41,-0.1 -0.586 127.2 25.6 -84.7 150.3 32.7 -72.1 7.8 48 53 A K + 0 0 171 -2,-0.2 -5,-0.3 -41,-0.2 -1,-0.2 0.922 70.0 175.7 60.4 47.7 35.5 -70.7 5.6 49 54 A F + 0 0 20 -3,-0.5 -6,-2.3 -44,-0.2 2,-0.3 0.916 68.8 13.1 -46.8 -50.1 34.9 -67.1 6.9 50 55 A G E S-F 42 0B 7 -8,-0.2 -8,-0.2 -23,-0.0 -43,-0.1 -0.861 74.3-142.0-128.1 165.8 37.9 -66.0 4.9 51 56 A V E -F 41 0B 53 -10,-1.9 -10,-1.8 -2,-0.3 2,-0.7 -0.944 26.1-106.2-132.1 144.7 40.0 -67.4 2.2 52 57 A P E +F 40 0B 92 0, 0.0 -12,-0.2 0, 0.0 3,-0.1 -0.606 45.1 164.3 -67.1 110.9 43.8 -67.3 1.2 53 58 A V E - 0 0 76 -14,-2.4 2,-0.3 -2,-0.7 -13,-0.2 0.859 58.0 -9.0 -95.2 -46.1 43.8 -64.9 -1.8 54 59 A R E -F 39 0B 101 -15,-1.4 -15,-3.0 2,-0.0 -1,-0.3 -0.989 51.6-147.6-156.8 144.4 47.5 -64.0 -2.1 55 60 A S E -F 38 0B 44 -2,-0.3 2,-0.5 -17,-0.2 -17,-0.2 -0.817 9.3-151.4-108.1 153.7 50.9 -64.4 -0.4 56 61 A F E -F 37 0B 2 -19,-2.1 -19,-1.3 -2,-0.3 2,-0.8 -0.935 11.2-173.6-127.9 105.7 53.7 -61.8 -0.6 57 62 A K + 0 0 175 -2,-0.5 -21,-0.1 -21,-0.2 -2,-0.0 -0.460 43.0 110.0-106.6 64.3 57.1 -63.5 -0.2 58 63 A G + 0 0 24 -2,-0.8 -1,-0.2 -23,-0.1 -22,-0.1 0.850 46.1 83.4-102.2 -41.5 59.6 -60.7 0.0 59 64 A H - 0 0 10 -24,-0.5 4,-0.1 -3,-0.3 3,-0.0 -0.306 59.8-145.5 -75.7 153.2 61.2 -60.2 3.4 60 65 A S S S+ 0 0 90 2,-0.1 2,-0.3 -2,-0.0 -1,-0.1 0.443 83.8 21.1 -89.5 -3.8 64.2 -62.3 4.6 61 66 A H S S- 0 0 72 -26,-0.1 20,-0.2 1,-0.1 -2,-0.1 -0.849 105.9 -35.4-152.1-175.0 62.8 -62.2 8.1 62 67 A I - 0 0 18 -2,-0.3 18,-3.4 -29,-0.2 2,-0.5 -0.158 46.3-138.3 -54.1 132.8 59.8 -61.7 10.3 63 68 A V E -G 79 0C 0 -31,-1.6 16,-0.3 16,-0.3 -47,-0.2 -0.822 19.3-172.2 -84.5 135.0 57.3 -59.2 9.2 64 69 A Q E + 0 0 45 14,-4.4 2,-0.3 -2,-0.5 15,-0.2 0.571 61.2 3.7-106.4 -13.3 56.3 -57.4 12.4 65 70 A D E -G 78 0C 36 13,-1.3 13,-2.6 42,-0.1 -1,-0.4 -0.951 57.5-170.0-172.4 146.4 53.5 -55.3 11.2 66 71 A C E +G 77 0C 2 -2,-0.3 2,-0.3 11,-0.2 11,-0.2 -0.921 6.4 173.7-138.6 162.5 51.3 -54.6 8.2 67 72 A T E -G 76 0C 38 9,-1.2 9,-1.0 -2,-0.3 2,-0.2 -0.900 19.7-131.3-172.3 150.1 48.7 -52.1 7.0 68 73 A L E -G 75 0C 14 -2,-0.3 7,-0.2 7,-0.2 42,-0.1 -0.659 30.2-101.1-103.6 154.2 46.7 -51.1 4.0 69 74 A T - 0 0 14 5,-2.0 -46,-0.3 -2,-0.2 -1,-0.1 -0.113 46.7 -98.8 -57.0 169.1 46.1 -47.8 2.3 70 75 A A S S+ 0 0 68 42,-0.2 -47,-1.3 1,-0.2 -48,-0.2 0.772 127.4 43.3 -68.8 -24.8 42.7 -46.2 3.0 71 76 A D S S- 0 0 86 -48,-0.2 -1,-0.2 -49,-0.2 -50,-0.1 0.637 114.0-117.8 -92.8 -18.4 41.3 -47.5 -0.3 72 77 A G S S+ 0 0 1 -49,-0.2 -2,-0.1 2,-0.2 17,-0.1 0.530 79.1 122.4 95.0 9.3 43.0 -50.9 0.2 73 78 A A + 0 0 30 1,-0.1 16,-1.9 16,-0.1 17,-0.4 0.900 67.8 37.1 -69.8 -44.2 45.1 -50.6 -2.9 74 79 A Y E - H 0 88C 83 14,-0.2 -5,-2.0 15,-0.1 2,-0.3 -0.922 64.6-151.5-125.7 138.0 48.5 -51.0 -1.3 75 80 A A E -GH 68 87C 0 12,-1.6 12,-3.2 -2,-0.4 2,-0.4 -0.712 10.5-156.0-104.0 155.7 49.9 -53.1 1.5 76 81 A L E -GH 67 86C 3 -9,-1.0 -9,-1.2 -2,-0.3 2,-0.4 -0.991 4.6-162.8-141.3 125.5 52.8 -52.2 3.6 77 82 A S E -GH 66 85C 0 8,-2.3 8,-0.6 -2,-0.4 2,-0.4 -0.870 7.2-163.3-115.0 140.2 55.2 -54.5 5.6 78 83 A A E -GH 65 84C 0 -13,-2.6 -14,-4.4 -2,-0.4 -13,-1.3 -0.989 26.5-174.2-120.5 131.8 57.6 -53.8 8.5 79 84 A S E > -G 63 0C 0 4,-1.8 26,-1.5 -2,-0.4 3,-1.5 -0.832 44.5-121.6-137.0 160.1 60.1 -56.5 9.1 80 85 A W T 3 S+ 0 0 48 -18,-3.4 -18,-0.1 1,-0.3 -17,-0.1 0.389 109.7 71.5 -78.7 -0.7 63.0 -57.8 11.2 81 86 A D T 3 S- 0 0 28 -19,-0.4 -1,-0.3 -20,-0.2 3,-0.1 0.375 117.3-118.8 -87.6 -0.4 65.1 -57.7 8.1 82 87 A K S < S+ 0 0 72 -3,-1.5 19,-0.3 1,-0.3 2,-0.2 0.740 74.4 125.1 74.7 23.6 64.8 -54.0 8.9 83 88 A T - 0 0 28 22,-0.1 -4,-1.8 17,-0.1 2,-0.4 -0.672 51.5-150.9-105.4 160.9 63.1 -53.4 5.6 84 89 A L E -HI 78 98C 0 14,-1.3 14,-3.2 -2,-0.2 2,-0.2 -0.870 17.4-167.7-131.4 107.8 59.9 -51.9 4.7 85 90 A R E -H 77 0C 45 -8,-0.6 -8,-2.3 -2,-0.4 2,-0.4 -0.608 9.2-151.9 -89.2 151.5 58.3 -53.2 1.5 86 91 A L E -H 76 0C 18 -2,-0.2 9,-3.6 -10,-0.2 2,-0.3 -0.987 22.0-167.6-117.6 131.3 55.4 -51.6 -0.3 87 92 A W E -HJ 75 94C 2 -12,-3.2 -12,-1.6 -2,-0.4 2,-0.7 -0.874 30.2-125.1-124.5 156.7 53.2 -53.9 -2.3 88 93 A D E >> -H 74 0C 27 5,-1.9 4,-3.3 -2,-0.3 3,-1.2 -0.884 25.7-149.9 -89.2 113.7 50.4 -53.9 -4.8 89 94 A V T 34 S+ 0 0 19 -16,-1.9 -1,-0.1 -2,-0.7 -15,-0.1 0.837 93.3 53.6 -54.2 -36.2 47.7 -56.0 -3.1 90 95 A A T 34 S+ 0 0 70 -17,-0.4 -1,-0.3 1,-0.1 -16,-0.1 0.700 125.0 19.4 -76.6 -21.5 46.4 -57.2 -6.5 91 96 A T T <4 S- 0 0 62 -3,-1.2 -2,-0.2 2,-0.2 -1,-0.1 0.733 88.8-127.3-117.9 -39.6 49.8 -58.5 -7.7 92 97 A G < + 0 0 22 -4,-3.3 2,-0.5 1,-0.3 -35,-0.2 0.618 58.4 144.9 89.9 15.1 52.1 -59.0 -4.8 93 98 A E - 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