==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=21-JUN-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER SIGNALING PROTEIN 06-APR-11 3RFH . COMPND 2 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-L . SOURCE 2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE; . AUTHOR L.YATIME,K.L.HEIN,J.NILSSON,P.NISSEN . 1176 9 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 49843.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 818 69.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 7 0.6 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 553 47.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 36 3.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 24 2.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 8 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 10 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 126 10.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 64 5.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 7 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 2 0 5 4 50 15 4 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 15 3 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A S 0 0 174 0, 0.0 2,-0.3 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 116.8 6.0 -10.5 72.9 2 4 A N - 0 0 103 1,-0.0 2,-0.1 290,-0.0 244,-0.0 -0.724 360.0-146.4-121.7 146.3 9.1 -8.8 71.7 3 5 A E - 0 0 60 -2,-0.3 2,-0.3 289,-0.2 289,-0.2 -0.387 24.7-148.6 -90.6 177.0 11.4 -8.0 68.7 4 6 A V E -A 291 0A 55 287,-1.8 287,-2.5 -2,-0.1 2,-0.5 -0.999 14.2-130.2-151.6 144.5 15.2 -7.6 68.9 5 7 A L E -A 290 0A 11 -2,-0.3 2,-0.5 285,-0.2 285,-0.2 -0.866 29.0-161.9 -95.3 128.0 18.1 -5.8 67.3 6 8 A V E -A 289 0A 50 283,-3.2 283,-2.0 -2,-0.5 2,-0.2 -0.941 23.6-116.3-119.9 124.6 20.9 -8.1 66.3 7 9 A L E -A 288 0A 73 -2,-0.5 281,-0.3 281,-0.2 3,-0.1 -0.348 32.5-174.7 -59.5 121.4 24.4 -7.0 65.5 8 10 A R - 0 0 64 279,-3.9 2,-0.2 -2,-0.2 280,-0.2 0.736 45.4 -77.2 -98.9 -26.5 24.9 -7.8 61.8 9 11 A G - 0 0 5 278,-0.8 278,-0.3 40,-0.1 2,-0.3 -0.810 53.0 -86.2 167.8-122.4 28.5 -7.1 61.1 10 12 A T - 0 0 17 40,-1.4 43,-0.6 -2,-0.2 42,-0.4 -0.955 13.0-136.5-177.0 161.3 30.6 -3.9 60.6 11 13 A L B +B 285 0A 2 274,-3.0 274,-2.4 -2,-0.3 2,-0.2 -0.873 25.0 178.6-137.4 102.8 31.7 -1.4 58.0 12 14 A E + 0 0 126 -2,-0.4 272,-0.1 41,-0.2 3,-0.1 -0.667 37.3 101.5-102.1 158.2 35.3 -0.2 58.0 13 15 A G + 0 0 43 1,-0.5 2,-0.2 270,-0.4 -1,-0.1 0.166 57.5 98.4 155.7 -19.1 37.0 2.3 55.7 14 16 A H - 0 0 9 269,-0.7 -1,-0.5 -3,-0.2 4,-0.1 -0.581 52.9-155.9 -93.8 157.3 37.2 5.6 57.5 15 17 A N S S+ 0 0 128 -2,-0.2 2,-0.3 2,-0.1 -1,-0.1 0.607 76.9 38.4-104.1 -18.3 40.1 7.0 59.4 16 18 A G S S- 0 0 11 1,-0.2 21,-0.2 267,-0.1 264,-0.1 -0.809 106.9 -51.5-129.1 170.0 38.2 9.2 61.8 17 19 A W - 0 0 82 -2,-0.3 19,-1.8 264,-0.2 2,-1.1 -0.011 52.3-123.0 -37.4 136.5 35.0 9.2 63.8 18 20 A V E +E 35 0B 0 262,-1.6 247,-0.4 266,-0.2 17,-0.3 -0.801 38.5 176.6 -86.6 100.3 32.0 8.4 61.7 19 21 A T E + 0 0 12 15,-1.2 2,-0.3 -2,-1.1 16,-0.2 0.630 55.2 18.8 -87.3 -19.9 30.1 11.6 62.4 20 22 A S E -E 34 0B 6 14,-1.2 14,-2.2 246,-0.1 2,-0.4 -0.984 56.0-160.2-154.8 140.9 27.0 11.2 60.2 21 23 A L E -E 33 0B 13 246,-0.5 2,-0.4 -2,-0.3 12,-0.2 -0.955 10.6-168.2-119.6 146.1 25.1 8.5 58.3 22 24 A A E -E 32 0B 7 10,-3.0 10,-2.4 -2,-0.4 2,-0.3 -0.990 1.1-163.6-136.5 144.4 22.7 8.9 55.5 23 25 A T E -E 31 0B 43 -2,-0.4 2,-0.4 8,-0.2 48,-0.4 -0.810 7.4-146.2-123.3 158.4 20.2 6.6 53.8 24 26 A S - 0 0 2 6,-0.7 247,-0.3 3,-0.5 6,-0.3 -0.994 9.1-157.7-130.7 135.7 18.3 6.6 50.5 25 27 A A S S+ 0 0 72 48,-0.5 49,-0.2 -2,-0.4 -1,-0.1 0.884 99.1 50.3 -74.5 -40.5 14.8 5.3 49.8 26 28 A G S S+ 0 0 56 47,-2.0 -1,-0.2 1,-0.3 48,-0.1 0.690 120.1 38.6 -70.4 -19.0 15.5 5.0 46.0 27 29 A Q > + 0 0 84 46,-0.3 3,-1.6 3,-0.1 -3,-0.5 -0.687 60.8 169.6-135.4 80.5 18.7 3.1 46.8 28 30 A P T 3 S+ 0 0 86 0, 0.0 -1,-0.1 0, 0.0 243,-0.1 0.737 82.1 59.9 -59.9 -24.3 18.3 0.7 49.8 29 31 A N T 3 S+ 0 0 101 -3,-0.1 16,-2.2 15,-0.1 2,-0.4 0.128 93.6 86.0 -91.9 21.1 21.7 -0.8 48.9 30 32 A L E < + F 0 44B 16 -3,-1.6 -6,-0.7 -6,-0.3 2,-0.4 -0.984 50.4 178.3-128.4 130.1 23.4 2.5 49.3 31 33 A L E -EF 23 43B 1 12,-1.5 12,-3.1 -2,-0.4 2,-0.4 -0.972 10.4-158.7-126.4 144.2 24.8 4.1 52.5 32 34 A L E +EF 22 42B 1 -10,-2.4 -10,-3.0 -2,-0.4 2,-0.3 -0.979 12.9 179.9-123.9 136.7 26.6 7.4 52.9 33 35 A S E -EF 21 41B 0 8,-2.2 8,-2.7 -2,-0.4 2,-0.3 -0.976 13.6-154.2-136.8 148.6 28.8 8.3 55.8 34 36 A A E -EF 20 40B 0 -14,-2.2 -14,-1.2 -2,-0.3 -15,-1.2 -0.882 17.4-172.7-121.0 156.8 30.9 11.3 56.8 35 37 A S E > -E 18 0B 0 4,-1.7 31,-1.8 -2,-0.3 3,-1.1 -0.924 42.3-112.0-146.8 162.3 34.0 11.4 59.0 36 38 A R T 3 S+ 0 0 86 -19,-1.8 29,-0.1 -2,-0.3 -19,-0.1 0.499 105.4 83.5 -73.9 -3.5 36.5 13.5 60.9 37 39 A D T 3 S- 0 0 34 -20,-0.3 -1,-0.3 2,-0.2 3,-0.1 0.287 114.5-110.6 -81.5 10.5 39.0 12.2 58.3 38 40 A K S < S+ 0 0 92 -3,-1.1 24,-0.4 1,-0.2 23,-0.4 0.534 84.5 94.3 76.8 9.2 37.6 15.0 56.1 39 41 A T - 0 0 10 27,-0.1 -4,-1.7 21,-0.1 2,-0.3 -0.789 61.5-138.7-126.4 169.2 35.9 12.7 53.7 40 42 A L E -FG 34 59B 0 19,-1.1 19,-2.7 -2,-0.3 2,-0.4 -0.919 19.2-151.2-118.2 152.8 32.5 11.0 52.9 41 43 A I E -FG 33 58B 7 -8,-2.7 -8,-2.2 -2,-0.3 2,-0.4 -0.991 5.2-150.3-128.7 132.7 32.1 7.5 51.8 42 44 A S E -FG 32 57B 3 15,-2.1 14,-2.6 -2,-0.4 15,-1.0 -0.815 23.6-151.9 -92.3 139.2 29.4 5.9 49.7 43 45 A W E -FG 31 55B 1 -12,-3.1 -12,-1.5 -2,-0.4 2,-0.7 -0.941 16.1-144.8-123.0 134.7 28.8 2.2 50.5 44 46 A K E -FG 30 54B 108 10,-2.1 10,-1.8 -2,-0.4 2,-0.6 -0.840 26.3-147.7 -89.0 116.1 27.5 -0.8 48.5 45 47 A L E + G 0 53B 11 -16,-2.2 8,-0.2 -2,-0.7 7,-0.1 -0.771 29.3 165.3 -92.8 121.7 25.4 -2.9 50.9 46 48 A T - 0 0 87 6,-2.1 7,-0.2 -2,-0.6 -1,-0.2 0.686 40.6-134.6-103.4 -26.1 25.5 -6.6 50.3 47 49 A G + 0 0 39 5,-2.3 5,-0.2 1,-0.1 6,-0.1 0.652 59.1 135.3 78.3 16.2 24.0 -7.7 53.6 48 50 A D - 0 0 103 4,-0.5 2,-0.4 3,-0.5 -1,-0.1 0.038 55.2 -65.0 -80.8-166.4 26.6 -10.4 54.0 49 51 A D S S- 0 0 102 2,-0.2 -40,-0.1 1,-0.1 -41,-0.0 -0.982 101.8 -23.3-144.4 128.8 28.7 -11.3 57.1 50 52 A Q S S+ 0 0 119 -2,-0.4 2,-3.2 -42,-0.1 -40,-1.4 -0.986 129.8 68.2 -96.2 2.0 30.6 -9.8 58.4 51 53 A K + 0 0 109 1,-0.2 -3,-0.5 -42,-0.2 -5,-0.3 -0.270 58.9 170.0 -71.2 57.8 31.3 -7.6 55.4 52 54 A F - 0 0 6 -2,-3.2 -5,-2.3 -42,-0.4 -6,-2.1 0.845 66.9 -5.4 -36.3 -47.4 27.9 -6.0 55.6 53 55 A G E -G 45 0B 1 -43,-0.6 -41,-0.2 -3,-0.3 -8,-0.2 -0.880 63.9-140.1-144.1 175.1 29.1 -3.5 53.0 54 56 A V E -G 44 0B 60 -10,-1.8 -10,-2.1 -2,-0.3 -41,-0.1 -0.991 33.4 -97.1-146.0 134.3 32.1 -2.3 51.1 55 57 A P E +G 43 0B 54 0, 0.0 -12,-0.2 0, 0.0 3,-0.1 -0.094 36.5 174.2 -45.9 140.6 33.4 1.2 50.2 56 58 A V E + 0 0 94 -14,-2.6 2,-0.3 1,-0.4 -13,-0.2 0.704 61.9 3.4-113.4 -51.5 32.5 2.3 46.7 57 59 A R E -G 42 0B 103 -15,-1.0 -15,-2.1 2,-0.0 2,-0.5 -0.998 56.9-141.0-144.4 141.3 33.6 5.9 46.4 58 60 A S E -G 41 0B 53 -2,-0.3 2,-0.7 -17,-0.2 -17,-0.2 -0.891 15.5-148.2-103.4 131.9 35.5 8.5 48.5 59 61 A F E +G 40 0B 0 -19,-2.7 -19,-1.1 -2,-0.5 2,-0.3 -0.856 22.8 179.7-105.2 105.7 34.4 12.1 48.4 60 62 A K + 0 0 146 -2,-0.7 37,-0.2 35,-0.4 -21,-0.1 -0.776 40.1 84.5-110.4 150.2 37.3 14.5 48.9 61 63 A G + 0 0 38 1,-0.5 -1,-0.1 -23,-0.4 2,-0.1 -0.033 53.7 112.9 154.7 -37.7 37.4 18.3 49.0 62 64 A H - 0 0 10 -24,-0.4 -1,-0.5 -3,-0.2 4,-0.1 -0.404 54.2-151.1 -64.1 134.8 36.5 19.3 52.5 63 65 A S S S+ 0 0 86 -2,-0.1 2,-0.3 2,-0.1 -1,-0.1 0.227 74.7 8.2 -91.1 14.1 39.5 20.9 54.2 64 66 A H S S- 0 0 69 -26,-0.2 20,-0.2 1,-0.0 -27,-0.1 -0.970 108.1 -19.2-175.8 174.1 38.4 19.8 57.7 65 67 A I - 0 0 15 -2,-0.3 18,-3.0 -29,-0.1 2,-0.6 0.086 48.6-142.0 -32.0 129.9 36.0 17.6 59.6 66 68 A V E +H 82 0C 0 -31,-1.8 -47,-0.3 16,-0.2 16,-0.3 -0.922 23.9 176.8 -94.0 120.4 32.8 16.6 57.9 67 69 A Q E + 0 0 45 14,-2.6 2,-0.3 -2,-0.6 15,-0.2 0.789 57.6 14.0 -95.9 -33.0 30.1 16.8 60.6 68 70 A D E +H 81 0C 34 13,-1.3 13,-3.0 40,-0.2 2,-0.3 -0.976 56.8 178.3-145.7 153.6 26.9 16.0 58.7 69 71 A C E -H 80 0C 6 -2,-0.3 2,-0.3 11,-0.3 11,-0.2 -0.985 1.9-177.0-153.0 159.9 25.7 14.6 55.4 70 72 A T E -H 79 0C 27 9,-2.1 9,-2.7 -2,-0.3 2,-0.3 -0.991 16.0-133.4-156.8 157.7 22.4 13.7 53.7 71 73 A L E -H 78 0C 14 -48,-0.4 7,-0.2 -2,-0.3 42,-0.1 -0.849 24.8-111.4-115.9 150.6 21.1 12.1 50.5 72 74 A T > - 0 0 21 5,-2.5 3,-1.5 -2,-0.3 -46,-0.2 -0.313 44.2-100.2 -65.1 160.7 18.5 13.2 48.0 73 75 A A T 3 S+ 0 0 86 1,-0.3 -47,-2.0 42,-0.2 -48,-0.5 0.856 126.1 47.1 -53.9 -35.3 15.4 10.9 48.0 74 76 A D T 3 S- 0 0 87 -49,-0.2 -1,-0.3 -48,-0.1 -50,-0.1 0.552 108.9-123.0 -85.5 -10.4 16.8 9.2 44.9 75 77 A G S < S+ 0 0 0 -3,-1.5 -2,-0.1 2,-0.2 3,-0.1 0.517 81.1 115.3 81.2 6.1 20.3 8.8 46.2 76 78 A A S S+ 0 0 30 1,-0.1 16,-2.3 16,-0.1 17,-0.5 0.803 72.7 36.2 -79.6 -30.5 21.8 10.7 43.3 77 79 A Y E - I 0 91C 85 14,-0.2 -5,-2.5 15,-0.1 2,-0.3 -0.964 64.7-158.7-133.5 142.7 23.1 13.6 45.2 78 80 A A E -HI 71 90C 0 12,-1.9 12,-3.1 -2,-0.4 2,-0.4 -0.859 3.7-154.6-117.7 150.4 24.7 14.1 48.6 79 81 A L E -HI 70 89C 4 -9,-2.7 -9,-2.1 -2,-0.3 2,-0.4 -0.980 13.4-169.2-120.5 139.5 25.0 17.1 50.9 80 82 A S E -HI 69 88C 0 8,-1.9 8,-1.5 -2,-0.4 2,-0.4 -0.976 13.6-151.3-131.3 147.4 27.7 17.6 53.5 81 83 A A E -HI 68 87C 0 -13,-3.0 -14,-2.6 -2,-0.4 -13,-1.3 -0.934 23.1-169.3-109.6 135.9 28.4 20.0 56.3 82 84 A S E > -H 66 0C 0 4,-2.0 26,-2.4 -2,-0.4 3,-1.1 -0.865 39.0-126.3-129.4 159.7 32.0 20.7 57.4 83 85 A W T 3 S+ 0 0 42 -18,-3.0 -18,-0.1 -2,-0.3 -17,-0.1 0.466 105.1 79.1 -75.3 -4.9 34.1 22.4 60.1 84 86 A D T 3 S- 0 0 27 -19,-0.5 -1,-0.3 -20,-0.2 3,-0.1 0.246 115.8-113.9 -85.2 13.1 35.7 24.3 57.2 85 87 A K S < S+ 0 0 78 -3,-1.1 18,-0.5 1,-0.2 19,-0.5 0.679 82.2 109.0 69.8 20.8 32.5 26.4 57.4 86 88 A T E - J 0 102C 21 16,-0.1 -4,-2.0 -4,-0.1 2,-0.4 -0.735 56.2-148.1-121.5 162.2 31.4 25.1 54.0 87 89 A L E -IJ 81 101C 3 14,-1.2 14,-2.2 -2,-0.2 2,-0.4 -0.990 15.2-156.7-126.5 144.9 28.8 22.8 52.5 88 90 A R E -IJ 80 100C 29 -8,-1.5 -8,-1.9 -2,-0.4 2,-0.5 -0.968 10.8-147.4-122.3 141.0 29.3 20.8 49.4 89 91 A L E -IJ 79 99C 16 10,-2.3 9,-2.8 -2,-0.4 10,-1.1 -0.915 29.0-161.8 -99.3 126.0 26.8 19.3 47.0 90 92 A W E -IJ 78 97C 1 -12,-3.1 -12,-1.9 -2,-0.5 2,-0.8 -0.858 26.1-129.7-115.9 143.9 28.1 16.0 45.6 91 93 A D E > -I 77 0C 19 5,-2.5 4,-2.4 -2,-0.3 5,-0.4 -0.816 24.6-154.7 -82.3 110.8 27.4 13.8 42.6 92 94 A V T 4 S+ 0 0 15 -16,-2.3 -1,-0.2 -2,-0.8 -15,-0.1 0.891 88.4 47.0 -56.6 -42.5 27.0 10.4 44.4 93 95 A A T 4 S+ 0 0 78 -17,-0.5 -1,-0.2 1,-0.2 -16,-0.1 0.890 124.2 28.8 -69.7 -40.7 28.0 8.4 41.3 94 96 A T T 4 S- 0 0 49 2,-0.2 -1,-0.2 -37,-0.0 -2,-0.2 0.514 94.8-134.2-101.7 -9.8 31.1 10.4 40.3 95 97 A G < + 0 0 10 -4,-2.4 -35,-0.4 1,-0.2 2,-0.3 0.649 60.7 128.9 66.6 17.3 32.1 11.6 43.7 96 98 A E - 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