==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=4-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 06-OCT-07 2RH7 . COMPND 2 MOLECULE: GREEN FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: RENILLA RENIFORMIS; . AUTHOR A.M.LOENING,T.D.FENN,S.S.GAMBHIR . 845 14 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 36163.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 647 76.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 48 5.7 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 398 47.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 11 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 107 12.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 56 6.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 23 2.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 4 0 11 1 0 4 13 5 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 7 A G 0 0 65 0, 0.0 2,-0.1 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 71.9 20.2 10.2 -8.6 2 8 A L - 0 0 67 77,-0.1 2,-0.3 74,-0.0 3,-0.0 -0.460 360.0-153.7-119.4 178.5 20.7 9.0 -12.1 3 9 A K - 0 0 135 -2,-0.1 28,-0.4 1,-0.1 3,-0.0 -0.976 36.4-111.5-149.9 142.7 21.9 9.8 -15.6 4 10 A E S S+ 0 0 107 -2,-0.3 27,-1.8 1,-0.2 2,-0.6 0.876 113.7 42.3 -51.6 -41.0 20.8 8.5 -19.0 5 11 A V E S-A 30 0A 67 25,-0.2 25,-0.2 26,-0.1 -1,-0.2 -0.936 81.6-171.0-110.0 112.4 24.1 6.7 -19.4 6 12 A M E -A 29 0A 0 23,-1.7 23,-1.1 -2,-0.6 2,-0.1 -0.881 13.7-143.3-109.9 129.0 25.3 5.0 -16.1 7 13 A P E -A 28 0A 47 0, 0.0 103,-2.6 0, 0.0 2,-0.3 -0.508 21.5-162.9 -77.4 156.9 28.7 3.5 -15.4 8 14 A T E -Ab 27 110A 2 19,-2.2 19,-2.0 101,-0.2 2,-0.4 -0.988 18.3-161.3-140.3 152.7 28.8 0.3 -13.3 9 15 A K E -Ab 26 111A 67 101,-2.6 103,-2.9 -2,-0.3 2,-0.5 -0.998 17.1-164.4-127.9 131.4 31.4 -1.7 -11.3 10 16 A I E -Ab 25 112A 3 15,-3.1 15,-2.3 -2,-0.4 2,-0.6 -0.970 9.2-166.2-125.3 122.8 30.5 -5.3 -10.5 11 17 A N E -Ab 24 113A 57 101,-3.0 103,-2.4 -2,-0.5 2,-0.5 -0.931 12.3-169.7-108.1 111.6 32.2 -7.5 -7.9 12 18 A L E -Ab 23 114A 0 11,-2.7 11,-3.0 -2,-0.6 2,-0.4 -0.862 7.9-170.0-104.9 128.6 31.4 -11.2 -8.2 13 19 A E E -Ab 22 115A 97 101,-2.7 103,-2.7 -2,-0.5 2,-0.3 -0.975 21.5-175.4-115.2 131.6 32.3 -13.8 -5.6 14 20 A G E -Ab 21 116A 12 7,-3.3 7,-2.1 -2,-0.4 2,-0.3 -0.860 32.4-179.8-135.5 158.4 31.8 -17.4 -6.6 15 21 A L E +Ab 20 117A 65 101,-2.1 103,-2.1 -2,-0.3 2,-0.6 -0.797 10.7 179.6-154.6 106.7 31.8 -21.1 -5.7 16 22 A V E > S-Ab 19 118A 8 3,-2.3 3,-2.3 -2,-0.3 2,-0.4 -0.955 73.1 -41.1-113.2 116.0 30.9 -23.6 -8.4 17 23 A G T 3 S- 0 0 16 101,-3.0 101,-0.0 -2,-0.6 0, 0.0 -0.577 126.5 -28.4 60.6-121.2 31.1 -27.1 -7.0 18 24 A D T 3 S+ 0 0 155 -2,-0.4 2,-0.8 2,-0.1 -1,-0.3 0.325 113.9 110.5-101.0 1.8 34.3 -26.9 -4.9 19 25 A H E < -A 16 0A 53 -3,-2.3 -3,-2.3 2,-0.0 2,-0.3 -0.727 51.9-161.2 -91.7 112.0 35.9 -24.2 -7.1 20 26 A A E +A 15 0A 58 -2,-0.8 2,-0.3 -5,-0.2 -5,-0.2 -0.694 22.0 157.9 -87.0 141.0 36.2 -20.8 -5.4 21 27 A F E -A 14 0A 3 -7,-2.1 -7,-3.3 -2,-0.3 2,-0.4 -0.986 28.6-149.0-159.7 158.8 36.7 -17.8 -7.6 22 28 A S E -AC 13 44A 9 22,-2.1 21,-2.6 -2,-0.3 22,-1.8 -0.985 17.7-177.5-135.3 142.6 36.3 -14.1 -7.8 23 29 A M E -AC 12 42A 4 -11,-3.0 -11,-2.7 -2,-0.4 2,-0.3 -0.953 9.8-155.7-139.3 159.3 35.5 -11.9 -10.8 24 30 A E E -AC 11 41A 96 17,-2.8 17,-2.6 -2,-0.3 2,-0.3 -0.977 5.1-169.6-135.6 150.0 35.1 -8.3 -11.7 25 31 A G E -AC 10 40A 6 -15,-2.3 -15,-3.1 -2,-0.3 2,-0.4 -0.997 1.9-171.1-138.4 143.0 33.3 -6.4 -14.4 26 32 A V E +AC 9 39A 68 13,-2.0 13,-3.0 -2,-0.3 2,-0.3 -0.994 30.5 112.2-131.6 139.7 33.3 -2.7 -15.5 27 33 A G E -AC 8 38A 17 -19,-2.0 -19,-2.2 -2,-0.4 2,-0.3 -0.906 42.6-119.7-172.0-160.6 31.0 -1.1 -18.0 28 34 A E E +AC 7 37A 99 9,-1.8 9,-2.8 -21,-0.3 2,-0.3 -0.946 20.7 175.1-159.2 173.7 28.2 1.2 -18.8 29 35 A G E -AC 6 36A 9 -23,-1.1 -23,-1.7 -2,-0.3 2,-0.6 -0.978 36.5 -94.6-172.1 178.0 24.8 1.3 -20.2 30 36 A N E >> -AC 5 35A 24 5,-2.1 4,-2.1 -2,-0.3 5,-1.5 -0.941 23.2-166.1-117.8 109.3 21.7 3.3 -21.1 31 37 A I T 45S+ 0 0 6 -27,-1.8 34,-0.4 -2,-0.6 -26,-0.1 0.491 89.8 47.4 -78.8 -4.6 19.0 3.1 -18.3 32 38 A L T 45S+ 0 0 98 -28,-0.5 35,-0.3 34,-0.1 -1,-0.2 0.782 119.1 36.7 -94.2 -42.8 16.3 4.5 -20.6 33 39 A E T 45S- 0 0 126 -29,-0.2 33,-0.5 33,-0.1 -2,-0.2 0.671 106.1-122.9 -86.6 -19.8 17.0 2.3 -23.7 34 40 A G T <5 + 0 0 1 -4,-2.1 172,-3.2 31,-0.4 2,-0.3 0.940 63.1 130.8 75.0 50.5 17.8 -0.8 -21.7 35 41 A T E < +CD 30 205A 38 -5,-1.5 -5,-2.1 170,-0.2 2,-0.3 -0.939 25.3 168.1-130.7 154.6 21.3 -1.5 -23.0 36 42 A Q E -CD 29 204A 10 168,-1.6 168,-2.4 -2,-0.3 2,-0.4 -0.949 18.5-159.3-164.6 146.5 24.6 -2.3 -21.3 37 43 A E E -CD 28 203A 91 -9,-2.8 -9,-1.8 -2,-0.3 2,-0.4 -0.998 14.6-173.1-130.9 130.7 28.1 -3.5 -22.1 38 44 A V E -CD 27 202A 4 164,-2.7 164,-2.4 -2,-0.4 2,-0.6 -0.982 17.5-146.7-129.7 130.0 30.4 -5.0 -19.4 39 45 A K E -CD 26 201A 127 -13,-3.0 -13,-2.0 -2,-0.4 2,-0.4 -0.864 27.5-163.5 -95.1 123.5 34.0 -6.1 -19.6 40 46 A I E +C 25 0A 4 160,-3.9 2,-0.3 -2,-0.6 -15,-0.2 -0.868 15.7 168.9-121.5 140.0 34.6 -9.1 -17.4 41 47 A S E -C 24 0A 41 -17,-2.6 -17,-2.8 -2,-0.4 2,-0.4 -0.985 35.8-112.6-144.6 149.5 37.7 -10.7 -15.9 42 48 A V E +C 23 0A 37 -2,-0.3 -19,-0.3 -19,-0.2 3,-0.1 -0.717 26.4 179.4 -80.9 133.3 38.6 -13.3 -13.3 43 49 A T E + 0 0 52 -21,-2.6 2,-0.3 -2,-0.4 -20,-0.2 0.491 69.7 17.1-108.6 -8.7 40.4 -11.8 -10.3 44 50 A K E S+C 22 0A 107 -22,-1.8 -22,-2.1 1,-0.0 -1,-0.3 -0.958 113.7 27.1-165.2 137.8 40.8 -15.0 -8.4 45 51 A G S S- 0 0 30 -2,-0.3 -1,-0.0 -24,-0.2 -22,-0.0 0.752 83.6-156.6 81.1 25.3 40.7 -18.7 -9.0 46 52 A A + 0 0 70 2,-0.1 -1,-0.2 1,-0.1 2,-0.1 -0.524 61.5 42.8 -80.2 151.6 41.6 -18.7 -12.7 47 53 A P S S- 0 0 113 0, 0.0 -1,-0.1 0, 0.0 -2,-0.0 0.654 82.6-135.8 -85.5 156.2 41.2 -20.5 -14.9 48 54 A L - 0 0 30 1,-0.1 4,-0.1 -2,-0.1 -2,-0.1 -0.494 0.4-146.9 -73.6 138.0 37.6 -21.5 -14.4 49 55 A P S S+ 0 0 50 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.232 79.6 54.1 -85.0 10.7 36.8 -25.2 -14.8 50 56 A F S S- 0 0 3 79,-0.1 78,-0.2 80,-0.1 2,-0.2 -0.873 101.4 -71.3-139.8 167.6 33.3 -24.4 -16.2 51 57 A A > - 0 0 3 76,-2.4 3,-1.2 -2,-0.3 4,-0.2 -0.446 35.0-139.9 -65.5 132.2 31.7 -22.3 -18.9 52 58 A F G > S+ 0 0 33 1,-0.2 3,-2.6 -2,-0.2 4,-0.3 0.889 91.8 77.5 -55.1 -43.2 31.9 -18.6 -18.3 53 59 A D G > S+ 0 0 6 1,-0.3 3,-1.3 2,-0.2 4,-0.4 0.645 75.5 73.8 -49.9 -23.9 28.3 -18.1 -19.6 54 60 A I G < S+ 0 0 0 -3,-1.2 3,-0.3 1,-0.3 -1,-0.3 0.821 99.5 46.3 -65.1 -25.5 26.7 -19.4 -16.4 55 61 A V G X> S+ 0 0 0 -3,-2.6 4,-1.9 -4,-0.2 3,-0.5 0.436 84.3 96.4 -91.5 2.4 27.7 -16.1 -14.6 56 62 A S T <4 S+ 0 0 5 -3,-1.3 -1,-0.2 -4,-0.3 -2,-0.1 0.898 85.3 47.9 -58.8 -41.4 26.5 -13.9 -17.4 57 63 A V T 34 S+ 0 0 24 -4,-0.4 -1,-0.3 -3,-0.3 -2,-0.1 0.740 107.9 59.1 -68.0 -24.4 23.1 -13.3 -15.7 58 64 A A T <4 0 0 3 -3,-0.5 -2,-0.2 1,-0.2 -1,-0.2 0.883 360.0 360.0 -73.8 -37.0 25.1 -12.5 -12.5 59 65 A F < 0 0 10 -4,-1.9 -1,-0.2 -3,-0.1 -2,-0.1 0.027 360.0 360.0 -65.7 360.0 27.0 -9.6 -14.0 60 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 61 69 A N > 0 0 22 0, 0.0 3,-1.9 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 98.6 21.0 -5.0 -13.1 62 70 A R T 3 + 0 0 14 1,-0.3 3,-0.4 2,-0.0 13,-0.2 0.498 360.0 85.6 -67.1 -5.1 17.5 -4.5 -14.1 63 71 A A T 3 S+ 0 0 0 1,-0.3 2,-1.6 12,-0.1 -1,-0.3 0.894 81.9 68.5 -59.6 -32.9 18.1 -0.7 -13.9 64 72 A Y S < S+ 0 0 2 -3,-1.9 2,-0.3 -30,-0.1 -1,-0.3 -0.457 78.7 106.9 -84.2 62.1 19.4 -1.3 -17.4 65 73 A T S S- 0 0 2 -2,-1.6 2,-0.7 -34,-0.4 -31,-0.4 -0.997 70.5-119.7-137.0 144.7 16.0 -2.1 -19.0 66 74 A G B -e 207 0A 8 140,-3.5 142,-3.1 -33,-0.5 -33,-0.1 -0.696 33.7-169.9 -79.3 114.8 13.8 -0.0 -21.4 67 75 A Y - 0 0 34 -2,-0.7 6,-0.1 -35,-0.3 142,-0.1 -0.934 16.8-134.2-113.7 124.1 10.5 0.6 -19.8 68 76 A P > - 0 0 32 0, 0.0 3,-1.7 0, 0.0 141,-0.0 -0.382 28.3-113.6 -65.8 148.4 7.5 2.1 -21.6 69 77 A E T 3 S+ 0 0 166 1,-0.3 4,-0.1 -2,-0.1 -2,-0.0 0.822 114.2 62.8 -53.5 -32.4 5.6 4.8 -19.8 70 78 A E T 3 S+ 0 0 137 1,-0.1 2,-0.5 2,-0.1 -1,-0.3 0.669 95.5 66.3 -72.3 -17.9 2.6 2.5 -19.5 71 79 A I S < S- 0 0 9 -3,-1.7 -1,-0.1 137,-0.1 137,-0.1 -0.924 90.8-115.6-111.7 121.0 4.4 -0.1 -17.4 72 80 A S - 0 0 27 -2,-0.5 2,-0.9 110,-0.1 3,-0.1 -0.307 22.7-141.9 -52.7 126.5 5.5 0.7 -13.8 73 81 A D > - 0 0 17 1,-0.2 4,-1.9 -4,-0.1 -1,-0.1 -0.812 19.4-177.4 -96.7 96.1 9.4 0.6 -13.7 74 82 A Y T 4 S+ 0 0 4 -2,-0.9 -1,-0.2 1,-0.2 4,-0.1 0.796 79.3 52.0 -63.7 -30.8 10.0 -1.0 -10.2 75 83 A F T >4 S+ 0 0 0 2,-0.2 3,-1.3 -13,-0.2 4,-0.4 0.929 109.1 44.4 -79.9 -47.5 13.8 -0.7 -10.7 76 84 A L G >4 S+ 0 0 53 1,-0.2 3,-1.4 -14,-0.2 -2,-0.2 0.924 111.1 58.8 -59.0 -40.0 14.1 3.0 -11.6 77 85 A Q G 3< S+ 0 0 40 -4,-1.9 -1,-0.2 1,-0.3 -2,-0.2 0.518 94.6 66.6 -66.2 -6.5 11.6 3.6 -8.8 78 86 A S G <> S+ 0 0 0 -3,-1.3 4,-2.1 -4,-0.1 3,-0.4 0.634 86.9 134.0 -93.3 -16.0 14.0 2.0 -6.2 79 87 A F T <4 + 0 0 12 -3,-1.4 -77,-0.1 -4,-0.4 25,-0.0 -0.420 44.0 34.7 -85.4 162.2 16.8 4.6 -6.4 80 88 A P T 4 S+ 0 0 72 0, 0.0 25,-0.4 0, 0.0 -1,-0.3 -0.981 133.5 37.4 -73.8 -16.2 18.7 6.4 -5.0 81 89 A E T 4 S- 0 0 98 -3,-0.4 24,-0.3 1,-0.2 -2,-0.3 0.915 98.9-160.3 -65.1 -44.3 18.8 3.5 -2.5 82 90 A G < - 0 0 0 -4,-2.1 94,-2.8 22,-0.1 95,-0.5 -0.611 8.5 -88.1 98.3-157.4 18.5 0.8 -5.2 83 91 A F E -FG 103 175A 0 20,-1.2 20,-2.9 92,-0.3 2,-0.3 -0.901 19.4-144.5-150.1 169.1 17.5 -2.9 -4.9 84 92 A T E -FG 102 174A 45 90,-2.6 90,-2.9 -2,-0.3 2,-0.3 -0.851 12.9-155.1-128.8 171.8 18.6 -6.4 -4.3 85 93 A Y E -FG 101 173A 7 16,-1.8 16,-2.1 -2,-0.3 2,-0.4 -0.994 14.7-154.0-146.8 155.7 17.7 -9.8 -5.6 86 94 A E E -FG 100 172A 81 86,-1.7 86,-3.2 -2,-0.3 2,-0.4 -1.000 25.6-172.7-120.9 126.2 17.7 -13.5 -4.7 87 95 A R E -FG 99 171A 2 12,-2.8 12,-2.5 -2,-0.4 2,-0.4 -0.970 10.6-164.2-130.0 126.6 17.8 -15.7 -7.8 88 96 A N E -FG 98 170A 54 82,-2.9 82,-2.5 -2,-0.4 2,-0.4 -0.957 8.3-167.4-114.5 137.0 17.4 -19.5 -8.0 89 97 A I E -FG 97 169A 2 8,-2.6 8,-2.1 -2,-0.4 2,-0.5 -0.976 9.3-168.1-127.9 131.5 18.4 -21.5 -11.1 90 98 A R E -FG 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22.7 -15.9 -6.8 100 108 A K E +FH 86 115A 97 15,-2.0 15,-3.0 -2,-0.4 2,-0.3 -0.980 10.7 179.6-128.9 137.1 22.3 -12.9 -4.5 101 109 A S E -FH 85 114A 10 -16,-2.1 -16,-1.8 -2,-0.4 2,-0.5 -0.995 23.3-153.6-145.8 145.5 23.0 -9.4 -5.4 102 110 A D E -FH 84 113A 54 11,-1.9 11,-1.9 -2,-0.3 2,-0.6 -0.984 10.5-166.3-115.9 124.3 23.1 -5.8 -4.2 103 111 A I E +FH 83 112A 0 -20,-2.9 -20,-1.2 -2,-0.5 2,-0.2 -0.947 21.2 165.3-109.3 111.7 22.6 -3.0 -6.6 104 112 A S E - H 0 111A 33 7,-2.3 7,-1.7 -2,-0.6 2,-0.4 -0.730 33.0-118.8-122.7 175.2 23.6 0.4 -5.0 105 113 A L E H 0 110A 39 -25,-0.4 5,-0.2 -24,-0.3 -103,-0.0 -0.965 360.0 360.0-126.4 125.1 24.3 3.9 -6.3 106 114 A E 0 0 146 3,-3.5 -1,-0.1 -2,-0.4 4,-0.1 0.789 360.0 360.0-104.1 360.0 27.6 5.6 -5.7 107 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 108 116 A G 0 0 63 0, 0.0 2,-0.4 0, 0.0 -105,-0.1 0.000 360.0 360.0 360.0 47.4 26.2 8.3 -10.2 109 117 A K - 0 0 56 -103,-0.1 -3,-3.5 -107,-0.0 2,-0.8 -0.991 360.0-126.2-122.1 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