==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=13-NOV-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER MEMBRANE PROTEIN 20-JAN-04 1S5H . COMPND 2 MOLECULE: ANTIBODY FAB FRAGMENT LIGHT CHAIN; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR R.MACKINNON,M.ZHOU . 533 3 3 3 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 24220.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 356 66.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 10 1.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 167 31.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 5 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 2 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 3 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 50 9.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 33 6.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 81 15.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 6 1.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 RESIDUES PER ALPHA HELIX . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 3 6 5 2 4 8 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 5 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A D 0 0 106 0, 0.0 2,-0.5 0, 0.0 95,-0.1 0.000 360.0 360.0 360.0 133.7 153.5 138.9 -12.7 2 2 A I - 0 0 5 93,-0.4 2,-0.5 91,-0.2 88,-0.1 -0.553 360.0-150.4 -76.5 119.3 150.4 141.0 -11.8 3 3 A L - 0 0 114 -2,-0.5 23,-2.1 94,-0.1 2,-0.6 -0.758 5.9-157.9 -90.3 128.9 149.5 140.9 -8.1 4 4 A L E -A 25 0A 6 -2,-0.5 96,-0.7 94,-0.4 2,-0.6 -0.948 6.2-158.0-110.3 116.3 145.7 141.3 -7.4 5 5 A T E -A 24 0A 73 19,-3.0 19,-2.3 -2,-0.6 2,-0.7 -0.856 7.9-172.2-100.7 118.2 145.0 142.5 -3.9 6 6 A Q - 0 0 6 -2,-0.6 17,-0.2 17,-0.2 -2,-0.0 -0.912 21.1-156.0-110.6 106.1 141.5 141.9 -2.5 7 7 A S + 0 0 78 -2,-0.7 15,-0.2 15,-0.3 2,-0.1 -1.000 65.6 35.3-139.6 137.0 141.0 143.6 0.8 8 8 A P S S- 0 0 63 0, 0.0 14,-0.2 0, 0.0 -1,-0.1 0.517 70.4-151.5 -74.7 161.0 139.3 143.2 3.1 9 9 A A S S+ 0 0 62 93,-0.1 94,-3.0 -2,-0.1 2,-0.4 0.697 83.2 35.9 -70.2 -22.7 139.2 139.5 3.1 10 10 A I E -d 103 0B 89 92,-0.2 2,-0.4 75,-0.0 94,-0.2 -0.999 66.6-165.7-137.1 138.0 135.8 139.5 4.7 11 11 A L E -d 104 0B 32 92,-2.9 94,-3.7 -2,-0.4 2,-0.5 -0.988 4.2-164.4-127.1 123.9 132.8 141.9 4.2 12 12 A S E +d 105 0B 79 -2,-0.4 2,-0.3 92,-0.2 94,-0.2 -0.915 17.6 163.3-109.9 132.5 129.8 142.0 6.6 13 13 A V E -d 106 0B 19 92,-2.1 94,-2.9 -2,-0.5 3,-0.1 -0.967 37.0-110.7-144.7 156.5 126.7 143.7 5.6 14 14 A S > - 0 0 71 -2,-0.3 3,-1.9 92,-0.2 64,-0.3 -0.603 54.4 -81.4 -86.1 151.4 123.0 143.8 6.7 15 15 A P T 3 S+ 0 0 81 0, 0.0 64,-0.2 0, 0.0 -1,-0.1 -0.239 115.0 18.1 -55.1 132.0 120.4 142.3 4.4 16 16 A G T 3 S+ 0 0 40 62,-2.4 61,-0.2 1,-0.3 63,-0.1 0.208 94.5 123.2 94.0 -16.4 119.3 144.7 1.6 17 17 A E < - 0 0 42 -3,-1.9 61,-2.3 60,-0.2 2,-0.4 -0.370 67.7-106.6 -78.6 158.9 122.3 147.0 1.8 18 18 A R - 0 0 135 59,-0.2 2,-0.3 58,-0.2 58,-0.2 -0.688 38.5-165.9 -85.3 135.1 124.7 147.8 -1.1 19 19 A V E - B 0 75A 9 56,-2.3 56,-2.5 -2,-0.4 2,-0.4 -0.935 7.5-159.0-126.0 148.7 128.1 146.1 -0.8 20 20 A S E - B 0 74A 54 -2,-0.3 2,-0.4 54,-0.2 54,-0.2 -0.989 3.5-155.5-132.7 128.8 131.4 146.7 -2.7 21 21 A F E - B 0 73A 1 52,-3.1 52,-1.9 -2,-0.4 2,-0.4 -0.834 17.4-139.1-101.8 136.4 134.4 144.4 -3.1 22 22 A S E + B 0 72A 53 -2,-0.4 2,-0.4 -15,-0.2 -15,-0.3 -0.769 19.7 178.8-100.7 142.3 137.8 145.9 -3.9 23 23 A a E - B 0 71A 0 48,-2.0 48,-2.5 -2,-0.4 2,-0.5 -0.962 6.4-172.3-139.7 116.3 140.4 144.6 -6.3 24 24 A R E -AB 5 70A 30 -19,-2.3 -19,-3.0 -2,-0.4 2,-0.3 -0.952 11.6-148.1-116.5 123.3 143.7 146.5 -6.7 25 25 A A E -A 4 0A 9 44,-2.8 -21,-0.2 -2,-0.5 4,-0.1 -0.673 18.9-129.2 -89.5 142.7 146.2 145.5 -9.4 26 26 A S S S+ 0 0 70 -23,-2.1 2,-0.3 -2,-0.3 -1,-0.1 0.609 94.2 12.6 -64.3 -13.4 150.0 145.9 -8.9 27 27 A Q S S- 0 0 72 -24,-0.2 2,-0.1 41,-0.1 -2,-0.0 -0.966 103.7 -69.1-155.8 163.5 150.2 147.8 -12.2 28 28 A S + 0 0 75 -2,-0.3 41,-0.2 1,-0.1 3,-0.1 -0.354 44.5 169.1 -62.4 129.5 147.7 149.3 -14.7 29 29 A I > - 0 0 1 39,-2.8 3,-2.3 1,-0.4 62,-0.2 -0.003 34.1-136.0-131.2 28.9 145.6 146.8 -16.6 30 30 A G T 3 S- 0 0 36 1,-0.3 38,-1.9 2,-0.1 -1,-0.4 -0.304 70.2 -37.2 52.9-121.6 143.0 148.9 -18.3 31 31 A T T 3 S+ 0 0 64 36,-0.2 2,-1.9 -3,-0.1 -1,-0.3 0.077 97.8 124.8-121.1 22.0 139.7 147.1 -17.9 32 32 A D < + 0 0 5 -3,-2.3 59,-1.7 18,-0.2 60,-0.4 -0.296 40.5 116.8 -80.8 53.3 141.0 143.5 -18.2 33 33 A I E -E 90 0B 0 -2,-1.9 18,-3.5 57,-0.2 2,-0.3 -0.957 42.6-169.2-122.6 142.0 139.5 142.4 -14.9 34 34 A H E -E 89 0B 0 55,-2.1 55,-1.6 -2,-0.4 2,-0.4 -0.968 11.0-141.1-130.6 147.3 136.9 139.7 -14.4 35 35 A W E -EF 88 48B 0 13,-2.6 12,-3.4 -2,-0.3 13,-1.3 -0.917 14.1-170.0-115.9 136.3 134.8 138.7 -11.4 36 36 A Y E -EF 87 46B 0 51,-2.9 51,-2.2 -2,-0.4 2,-0.5 -0.928 12.2-146.7-123.9 147.7 133.8 135.2 -10.2 37 37 A Q E -EF 86 45B 7 8,-2.9 8,-1.3 -2,-0.3 2,-0.5 -0.939 13.8-174.6-112.3 129.0 131.4 134.0 -7.5 38 38 A Q E -E 85 0B 13 47,-2.9 47,-2.8 -2,-0.5 2,-0.1 -0.932 5.8-163.6-128.8 110.9 132.2 130.8 -5.7 39 39 A R > - 0 0 81 -2,-0.5 3,-2.7 4,-0.4 45,-0.1 -0.434 48.3 -64.0 -83.8 163.1 129.6 129.4 -3.2 40 40 A T T 3 S- 0 0 81 1,-0.3 -1,-0.2 43,-0.3 3,-0.1 -0.214 122.2 -5.7 -47.7 127.1 130.5 126.7 -0.6 41 41 A N T 3 S+ 0 0 163 1,-0.2 -1,-0.3 -3,-0.1 2,-0.2 0.653 112.4 115.5 58.5 18.0 131.5 123.5 -2.4 42 42 A G < - 0 0 26 -3,-2.7 -1,-0.2 1,-0.0 3,-0.1 -0.660 69.1-107.2-110.5 169.9 130.6 125.0 -5.8 43 43 A S - 0 0 44 -2,-0.2 -4,-0.4 1,-0.1 -6,-0.1 -0.618 49.0 -82.7 -93.1 158.4 132.8 125.8 -8.8 44 44 A P - 0 0 4 0, 0.0 2,-0.4 0, 0.0 276,-0.2 -0.218 45.1-156.4 -57.5 148.7 133.6 129.5 -9.7 45 45 A R E -F 37 0B 124 -8,-1.3 -8,-2.9 274,-0.1 2,-0.2 -0.981 18.0-117.0-135.8 125.0 131.0 131.3 -11.7 46 46 A L E +F 36 0B 13 -2,-0.4 -10,-0.2 272,-0.3 3,-0.1 -0.356 37.9 163.6 -61.3 125.4 131.6 134.3 -14.0 47 47 A L E + 0 0 2 -12,-3.4 8,-0.7 1,-0.4 2,-0.3 0.775 65.2 9.3-108.8 -45.4 129.7 137.5 -12.8 48 48 A I E -FG 35 54B 1 -13,-1.3 -13,-2.6 6,-0.2 -1,-0.4 -0.997 63.7-157.2-141.1 138.5 131.3 140.4 -14.6 49 49 A K E >> S+ G 0 53B 53 4,-2.4 4,-2.0 -2,-0.3 3,-0.6 -0.897 74.2 10.1-119.0 151.7 133.7 140.5 -17.5 50 50 A Y T 34 S- 0 0 37 -2,-0.3 2,-2.1 1,-0.3 -16,-0.2 0.917 123.4 -70.5 46.8 51.8 136.1 143.3 -18.5 51 51 A A T 34 S+ 0 0 1 -18,-3.5 -1,-0.3 -3,-0.2 21,-0.2 -0.099 127.5 19.3 65.7 -38.1 135.4 145.2 -15.3 52 52 A S T <4 S+ 0 0 73 -2,-2.1 2,-0.4 -3,-0.6 12,-0.3 0.476 85.0 115.6-140.9 -3.5 131.8 146.3 -16.0 53 53 A E E < -G 49 0B 99 -4,-2.0 -4,-2.4 -3,-0.2 -2,-0.1 -0.611 59.1-131.3 -79.5 125.7 130.2 144.1 -18.7 54 54 A S E -G 48 0B 97 -2,-0.4 2,-0.4 -6,-0.2 -6,-0.2 -0.319 11.2-138.4 -73.9 154.8 127.3 142.1 -17.5 55 55 A I > - 0 0 34 -8,-0.7 3,-1.4 4,-0.1 2,-0.2 -0.900 26.6-106.1-113.2 142.4 126.8 138.4 -18.2 56 56 A S T 3 S+ 0 0 115 -2,-0.4 0, 0.0 1,-0.2 0, 0.0 -0.472 104.8 30.0 -71.3 136.3 123.5 136.9 -19.1 57 57 A G T 3 S+ 0 0 68 1,-0.3 -1,-0.2 -2,-0.2 3,-0.0 0.163 92.8 114.1 102.4 -17.1 121.9 134.8 -16.4 58 58 A I S < S- 0 0 27 -3,-1.4 -1,-0.3 -11,-0.1 4,-0.1 -0.769 76.9-102.8 -91.6 127.5 123.4 137.0 -13.7 59 59 A P > - 0 0 46 0, 0.0 3,-1.4 0, 0.0 -4,-0.1 -0.032 25.0-121.6 -45.9 147.7 121.0 139.1 -11.5 60 60 A S T 3 S+ 0 0 104 1,-0.3 -2,-0.0 -6,-0.1 16,-0.0 0.656 104.4 76.2 -68.3 -18.0 120.9 142.8 -12.3 61 61 A R T 3 S+ 0 0 42 14,-0.1 15,-2.8 16,-0.1 2,-0.5 0.583 80.5 88.2 -71.7 -6.8 121.9 143.8 -8.8 62 62 A F E < +C 75 0A 13 -3,-1.4 2,-0.3 13,-0.2 13,-0.2 -0.814 53.5 152.4 -95.2 127.6 125.4 142.8 -9.8 63 63 A S E -C 74 0A 48 11,-2.4 11,-3.6 -2,-0.5 2,-0.3 -0.979 24.3-153.5-149.2 158.5 127.7 145.5 -11.4 64 64 A G E +C 73 0A 11 -12,-0.3 2,-0.3 -2,-0.3 9,-0.2 -0.917 13.4 174.5-132.9 160.1 131.3 146.3 -11.7 65 65 A S E +C 72 0A 60 7,-2.3 7,-2.7 -2,-0.3 2,-0.1 -0.957 25.1 74.4-155.5 172.1 133.4 149.4 -12.2 66 66 A G E -C 71 0A 37 -2,-0.3 2,-0.3 5,-0.2 5,-0.2 -0.260 38.2-149.0 106.5 165.5 136.9 150.9 -12.3 67 67 A S E > +C 70 0A 80 3,-0.8 2,-0.9 -2,-0.1 3,-0.9 -0.948 63.2 28.6-165.1 158.6 139.9 150.9 -14.7 68 68 A G T 3 S- 0 0 17 -38,-1.9 -39,-2.8 -2,-0.3 -41,-0.1 -0.751 128.2 -22.8 90.3-105.3 143.6 151.2 -14.3 69 69 A T T 3 S+ 0 0 55 -2,-0.9 -44,-2.8 -41,-0.2 2,-0.5 0.546 122.8 71.2-118.1 -17.4 144.6 149.6 -11.0 70 70 A D E < +BC 24 67A 99 -3,-0.9 -3,-0.8 -46,-0.2 2,-0.3 -0.916 54.6 170.6-114.9 125.7 141.4 149.9 -9.0 71 71 A F E -BC 23 66A 1 -48,-2.5 -48,-2.0 -2,-0.5 2,-0.4 -0.927 13.4-158.0-130.5 153.6 138.3 147.9 -9.5 72 72 A T E -BC 22 65A 40 -7,-2.7 -7,-2.3 -2,-0.3 2,-0.5 -0.992 8.4-160.5-136.4 136.5 135.0 147.3 -7.7 73 73 A L E -BC 21 64A 0 -52,-1.9 -52,-3.1 -2,-0.4 2,-0.4 -0.972 24.3-167.8-111.1 122.5 132.4 144.6 -7.7 74 74 A S E -BC 20 63A 4 -11,-3.6 -11,-2.4 -2,-0.5 2,-0.5 -0.897 16.9-163.2-118.0 146.0 129.1 145.9 -6.3 75 75 A I E -BC 19 62A 0 -56,-2.5 -56,-2.3 -2,-0.4 2,-0.7 -0.992 16.0-145.6-123.7 120.1 125.9 144.2 -5.3 76 76 A N S S- 0 0 84 -15,-2.8 -58,-0.2 -2,-0.5 -59,-0.1 -0.785 81.3 -5.0 -88.9 117.5 122.8 146.3 -4.9 77 77 A S S S- 0 0 59 -2,-0.7 -1,-0.3 -61,-0.2 -59,-0.2 0.985 87.5-137.3 62.7 83.4 120.7 145.0 -2.1 78 78 A V - 0 0 3 -61,-2.3 -62,-2.4 -3,-0.3 2,-0.3 -0.413 23.7-173.5 -72.8 144.0 122.5 141.9 -1.0 79 79 A E > - 0 0 90 -64,-0.2 3,-2.3 -2,-0.1 4,-0.3 -0.847 41.7 -88.2-131.4 170.3 120.5 138.8 -0.2 80 80 A S G > S+ 0 0 30 1,-0.3 3,-2.0 -2,-0.3 90,-0.2 0.806 120.1 61.9 -47.9 -41.5 121.2 135.3 1.2 81 81 A E G 3 S+ 0 0 70 1,-0.3 -1,-0.3 88,-0.2 87,-0.1 0.680 93.5 67.0 -63.8 -15.3 122.1 133.7 -2.1 82 82 A D G < S+ 0 0 0 -3,-2.3 2,-0.3 2,-0.0 -1,-0.3 0.608 70.3 116.2 -80.7 -10.8 124.9 136.2 -2.5 83 83 A I < + 0 0 14 -3,-2.0 2,-0.3 -4,-0.3 -43,-0.3 -0.439 49.4 90.6 -62.5 119.2 126.8 134.6 0.4 84 84 A A S S- 0 0 8 20,-0.4 20,-2.4 -2,-0.3 2,-0.4 -0.966 79.1 -69.3 176.3-174.3 129.9 133.2 -1.1 85 85 A N E -EH 38 103B 62 -47,-2.8 -47,-2.9 -2,-0.3 2,-0.5 -0.831 41.2-151.0-102.3 143.7 133.6 134.0 -2.0 86 86 A Y E -EH 37 102B 0 16,-2.4 16,-2.1 -2,-0.4 2,-0.4 -0.968 12.7-173.3-123.4 125.1 134.4 136.6 -4.7 87 87 A Y E -E 36 0B 5 -51,-2.2 -51,-2.9 -2,-0.5 2,-0.3 -0.895 12.1-153.8-118.8 144.4 137.5 136.5 -6.8 88 88 A a E -E 35 0B 0 -2,-0.4 11,-2.6 -53,-0.2 2,-0.3 -0.761 14.9-169.2-107.5 159.5 138.9 138.9 -9.4 89 89 A Q E -EI 34 98B 0 -55,-1.6 -55,-2.1 -2,-0.3 2,-0.4 -0.990 10.2-141.0-150.3 138.3 141.2 137.8 -12.2 90 90 A Q E +EI 33 97B 0 7,-2.4 7,-1.1 -2,-0.3 -57,-0.2 -0.831 20.7 173.8-109.8 146.5 143.3 139.6 -14.7 91 91 A S + 0 0 1 -59,-1.7 380,-0.1 -2,-0.4 -58,-0.1 0.033 56.4 99.6-130.6 23.4 144.0 138.9 -18.3 92 92 A N S S+ 0 0 10 -60,-0.4 2,-0.3 1,-0.2 379,-0.2 0.897 91.7 17.5 -77.8 -41.5 146.0 142.0 -19.3 93 93 A R S S- 0 0 68 2,-0.2 -1,-0.2 377,-0.1 376,-0.2 -0.955 99.1 -66.8-132.8 152.1 149.4 140.3 -19.1 94 94 A W S S+ 0 0 4 374,-1.9 373,-2.8 371,-0.4 374,-0.2 -0.614 106.9 42.6 -81.2 136.6 150.6 136.7 -19.1 95 95 A P S S- 0 0 2 0, 0.0 -93,-0.4 0, 0.0 -2,-0.2 0.569 85.5-130.1 -89.9 162.4 150.1 134.8 -16.9 96 96 A F - 0 0 2 -5,-0.1 2,-0.3 -2,-0.1 -5,-0.2 -0.376 33.8-165.5 -62.1 147.1 146.5 135.3 -15.9 97 97 A T E -I 90 0B 26 -7,-1.1 -7,-2.4 -4,-0.1 2,-0.3 -0.962 12.4-139.1-138.8 158.9 146.4 135.7 -12.2 98 98 A F E -I 89 0B 26 -2,-0.3 -94,-0.4 159,-0.2 -9,-0.2 -0.871 17.9-121.7-117.9 149.7 143.7 135.6 -9.4 99 99 A G - 0 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