==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=12-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER BIOTIN-BINDING PROTEIN 27-JAN-98 1SWL . COMPND 2 MOLECULE: CORE-STREPTAVIDIN; . SOURCE 2 ORGANISM_SCIENTIFIC: STREPTOMYCES AVIDINII; . AUTHOR S.FREITAG,I.LE TRONG,A.CHILKOTI,L.A.KLUMB,P.S.STAYTON, . 455 7 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19000.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 317 69.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 240 52.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 2 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 1.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 44 9.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 26 5.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 2 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 3 3 0 5 6 4 1 0 8 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 16 A G 0 0 72 0, 0.0 86,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 -40.2 14.3 27.2 12.6 2 17 A I + 0 0 12 79,-0.1 14,-0.2 16,-0.1 87,-0.0 0.835 360.0 97.8 -73.8 -32.7 15.4 23.9 14.1 3 18 A T + 0 0 63 15,-0.1 2,-0.3 12,-0.1 14,-0.2 -0.289 65.6 65.1 -58.8 133.7 17.3 25.8 16.9 4 19 A G E S-A 16 0A 22 12,-2.2 12,-2.1 2,-0.0 2,-0.5 -0.943 88.7 -51.8 150.8-173.1 15.1 26.0 20.0 5 20 A T E -A 15 0A 51 -2,-0.3 2,-0.3 10,-0.2 10,-0.2 -0.915 51.8-172.1-104.4 127.7 13.4 24.1 22.8 6 21 A W E -A 14 0A 0 8,-3.1 8,-2.8 -2,-0.5 2,-0.4 -0.911 8.8-161.5-124.4 148.8 11.2 21.2 21.6 7 22 A Y E -AB 13 116A 59 109,-2.4 109,-2.2 -2,-0.3 6,-0.2 -0.987 9.6-143.4-128.0 142.3 8.8 18.9 23.5 8 23 A N E > - B 0 115A 13 4,-1.6 3,-1.5 -2,-0.4 107,-0.2 -0.351 38.9 -94.0 -93.9 179.1 7.3 15.6 22.6 9 24 A Q T 3 S+ 0 0 109 105,-0.6 106,-0.1 1,-0.3 -1,-0.1 0.580 123.0 57.1 -71.0 -2.0 3.9 14.3 23.3 10 25 A L T 3 S- 0 0 71 2,-0.1 -1,-0.3 22,-0.0 103,-0.0 0.454 121.1 -98.7-106.0 -2.3 5.1 12.6 26.5 11 26 A G S < S+ 0 0 28 -3,-1.5 2,-0.2 1,-0.3 -2,-0.1 0.642 73.2 143.6 97.8 14.4 6.5 15.7 28.2 12 27 A S - 0 0 0 18,-0.1 -4,-1.6 19,-0.0 2,-0.4 -0.633 41.0-134.6 -91.3 156.3 10.2 15.3 27.3 13 28 A T E -AC 7 29A 31 16,-2.1 16,-3.1 -2,-0.2 2,-0.4 -0.917 15.3-170.8-117.7 138.4 12.4 18.3 26.4 14 29 A F E -AC 6 28A 1 -8,-2.8 -8,-3.1 -2,-0.4 2,-0.6 -0.988 2.9-174.2-133.2 103.7 14.9 18.6 23.6 15 30 A I E +AC 5 27A 55 12,-2.3 12,-2.7 -2,-0.4 2,-0.3 -0.955 23.5 163.0-103.1 110.4 17.1 21.7 23.7 16 31 A V E -AC 4 26A 3 -12,-2.1 -12,-2.2 -2,-0.6 2,-0.4 -0.958 32.7-151.3-136.9 154.0 19.2 21.8 20.4 17 32 A T E - C 0 25A 73 8,-2.7 8,-2.4 -2,-0.3 2,-0.7 -0.992 11.5-150.9-126.3 123.2 21.3 24.1 18.3 18 33 A A E - C 0 24A 20 -2,-0.4 6,-0.2 6,-0.2 -15,-0.1 -0.807 20.3-160.2 -91.5 107.8 21.6 23.7 14.5 19 34 A G > - 0 0 23 4,-2.6 3,-1.1 -2,-0.7 -16,-0.0 -0.311 30.0-108.4 -81.8 172.3 25.0 25.0 13.4 20 35 A A T 3 S+ 0 0 99 1,-0.2 -1,-0.1 2,-0.1 27,-0.0 0.624 113.0 66.4 -79.4 -10.1 25.9 26.0 9.9 21 36 A D T 3 S- 0 0 107 2,-0.1 -1,-0.2 1,-0.0 3,-0.1 0.276 123.7 -97.8 -89.6 10.3 28.1 22.9 9.4 22 37 A G S < S+ 0 0 2 -3,-1.1 23,-1.8 1,-0.3 2,-0.2 0.505 81.4 142.5 83.3 6.4 25.1 20.7 9.5 23 38 A A E - D 0 44A 18 21,-0.2 -4,-2.6 19,-0.0 2,-0.4 -0.501 37.6-160.4 -85.9 152.9 25.8 19.9 13.1 24 39 A L E +CD 18 43A 0 19,-2.2 19,-1.9 -6,-0.2 2,-0.4 -0.988 16.2 173.6-127.2 118.9 23.2 19.5 15.8 25 40 A T E +CD 17 42A 73 -8,-2.4 -8,-2.7 -2,-0.4 2,-0.3 -0.977 19.3 91.1-133.2 147.0 24.2 19.9 19.5 26 41 A G E -CD 16 41A 23 15,-1.6 15,-2.3 -2,-0.4 2,-0.3 -0.971 56.9 -68.0 172.7-155.2 22.2 19.9 22.7 27 42 A T E -CD 15 40A 33 -12,-2.7 -12,-2.3 -2,-0.3 2,-0.4 -0.968 27.7-154.2-141.6 139.8 20.7 18.3 25.7 28 43 A Y E -CD 14 39A 1 11,-3.3 11,-2.2 -2,-0.3 2,-0.5 -0.990 4.1-168.7-120.5 136.1 17.8 15.9 26.1 29 44 A E E -CD 13 38A 81 -16,-3.1 -16,-2.1 -2,-0.4 2,-0.3 -0.981 15.4-153.6-122.0 118.5 15.6 15.4 29.2 30 45 A S - 0 0 23 7,-0.5 7,-0.2 -2,-0.5 -18,-0.1 -0.713 16.7-164.1 -96.1 143.9 13.3 12.3 29.1 31 46 A A S S+ 0 0 61 -2,-0.3 2,-0.3 5,-0.1 -1,-0.1 0.543 78.5 48.1 -93.7 -17.0 10.0 12.0 31.0 32 47 A V S S+ 0 0 39 2,-0.1 2,-0.1 416,-0.0 -2,-0.1 -0.834 90.5 24.8-123.5 161.4 10.0 8.2 30.6 33 48 A G S S- 0 0 48 -2,-0.3 2,-1.5 1,-0.1 416,-0.0 -0.272 103.9 -29.8 86.3-171.0 12.4 5.3 31.1 34 49 A N S S+ 0 0 88 37,-0.1 2,-0.3 -2,-0.1 37,-0.2 -0.664 102.4 97.2 -81.8 80.4 15.5 4.7 33.2 35 50 A A - 0 0 20 -2,-1.5 2,-0.3 35,-0.1 3,-0.1 -0.984 43.7-172.7-170.0 160.0 16.7 8.3 33.4 36 51 A E - 0 0 84 -2,-0.3 32,-0.3 33,-0.1 33,-0.2 -0.974 57.6 -3.4-162.0 139.4 16.9 11.6 35.3 37 52 A S S S- 0 0 47 -2,-0.3 -7,-0.5 -7,-0.2 2,-0.3 -0.308 99.5 -25.5 66.9-158.8 18.1 15.0 34.5 38 53 A R E -D 29 0A 133 -9,-0.1 2,-0.3 -3,-0.1 -9,-0.2 -0.685 47.7-168.2-101.0 145.4 19.9 16.0 31.3 39 54 A Y E -D 28 0A 4 -11,-2.2 -11,-3.3 -2,-0.3 2,-0.2 -0.933 28.7-108.1-126.8 148.0 21.9 14.0 28.7 40 55 A V E -D 27 0A 69 -2,-0.3 23,-0.5 25,-0.3 2,-0.3 -0.481 34.1-168.6 -74.4 140.7 24.1 15.2 25.8 41 56 A L E +DE 26 62A 2 -15,-2.3 -15,-1.6 -2,-0.2 2,-0.3 -0.957 8.0 179.4-124.1 154.2 22.9 14.8 22.3 42 57 A T E +DE 25 61A 22 19,-1.8 19,-2.1 -2,-0.3 2,-0.3 -0.998 15.3 130.8-149.8 153.6 24.6 15.2 19.0 43 58 A G E -DE 24 60A 0 -19,-1.9 -19,-2.2 -2,-0.3 2,-0.3 -0.931 38.7-101.7-168.2-163.6 23.5 14.8 15.3 44 59 A R E -DE 23 59A 28 15,-1.4 15,-2.0 -2,-0.3 2,-0.3 -0.904 21.9-171.1-138.9 158.5 23.4 16.1 11.8 45 60 A Y E - E 0 58A 31 -23,-1.8 2,-0.7 -2,-0.3 13,-0.2 -0.982 39.3 -91.2-148.3 162.2 20.9 17.8 9.5 46 61 A D - 0 0 3 11,-2.4 -24,-0.1 -2,-0.3 145,-0.1 -0.641 34.9-168.2 -74.0 109.0 20.5 18.8 5.9 47 62 A S S S+ 0 0 46 -2,-0.7 -1,-0.2 1,-0.3 -25,-0.1 0.556 81.1 35.2 -83.6 -1.0 21.8 22.4 5.9 48 63 A A S S- 0 0 47 -26,-0.0 -1,-0.3 -3,-0.0 141,-0.1 -0.514 82.2-178.4-148.2 78.2 20.6 23.0 2.4 49 64 A P - 0 0 54 0, 0.0 7,-0.1 0, 0.0 6,-0.0 -0.276 37.8 -73.8 -77.7 163.8 17.3 21.2 1.7 50 65 A A - 0 0 28 5,-0.3 3,-0.2 1,-0.1 4,-0.1 -0.141 30.3-140.5 -48.5 142.6 15.2 21.1 -1.5 51 66 A T S S+ 0 0 146 1,-0.2 2,-1.1 2,-0.1 -1,-0.1 0.554 82.0 92.9 -86.5 -13.6 13.2 24.2 -2.4 52 67 A D S S- 0 0 101 1,-0.1 -1,-0.2 3,-0.1 3,-0.1 -0.158 111.4 -88.6 -79.2 41.6 10.2 22.2 -3.5 53 68 A G S S+ 0 0 55 -2,-1.1 165,-0.3 1,-0.2 -1,-0.1 0.553 85.5 133.3 70.8 3.7 8.2 22.1 -0.2 54 69 A S - 0 0 31 163,-0.1 163,-0.3 161,-0.1 -1,-0.2 -0.438 54.9-109.7 -80.8 164.9 9.9 19.0 1.2 55 70 A G - 0 0 11 161,-2.4 2,-0.7 159,-0.1 -5,-0.3 -0.314 30.0 -94.0 -91.2 178.2 11.1 18.9 4.7 56 71 A T E - F 0 81A 18 25,-1.8 25,-2.3 -7,-0.1 2,-0.1 -0.826 34.5-135.6 -95.8 108.8 14.6 18.9 6.2 57 72 A A E + F 0 80A 0 -2,-0.7 -11,-2.4 23,-0.2 2,-0.3 -0.382 36.8 156.5 -67.3 139.6 16.1 15.5 7.0 58 73 A L E -EF 45 79A 1 21,-2.5 21,-2.5 -13,-0.2 2,-0.3 -0.965 18.5-175.4-151.0 167.7 17.9 15.2 10.4 59 74 A G E -EF 44 78A 0 -15,-2.0 -15,-1.4 -2,-0.3 2,-0.3 -0.972 2.4-178.6-160.2 162.4 18.9 12.7 13.1 60 75 A W E -EF 43 77A 0 17,-1.7 17,-2.4 -2,-0.3 2,-0.3 -0.966 13.4-139.8-158.6 166.0 20.5 12.6 16.5 61 76 A T E -EF 42 76A 0 -19,-2.1 -19,-1.8 -2,-0.3 2,-0.4 -0.958 6.2-168.9-130.0 144.1 21.7 10.2 19.3 62 77 A V E -EF 41 75A 0 13,-1.9 13,-2.3 -2,-0.3 2,-0.5 -0.995 10.6-155.5-128.6 124.8 21.6 10.2 23.1 63 78 A A E - F 0 74A 4 -23,-0.5 2,-2.1 -2,-0.4 11,-0.3 -0.901 26.0-129.5 -94.0 124.0 23.6 7.6 25.1 64 79 A W E S+ 0 0 21 9,-2.8 7,-2.3 -2,-0.5 2,-0.4 -0.265 70.5 110.7 -84.0 57.6 21.8 7.3 28.4 65 80 A K E + F 0 70A 49 -2,-2.1 -25,-0.3 5,-0.2 2,-0.2 -0.999 32.1 141.2-123.2 127.1 24.8 7.8 30.7 66 81 A N - 0 0 25 3,-2.1 -27,-0.1 -2,-0.4 -29,-0.0 -0.754 67.9 -76.4-142.0-163.9 25.1 11.0 32.8 67 82 A N S S+ 0 0 162 -2,-0.2 3,-0.1 1,-0.2 -2,-0.0 0.701 126.3 49.5 -76.0 -13.2 26.4 11.8 36.3 68 83 A Y S S- 0 0 143 1,-0.3 2,-0.3 -32,-0.3 -1,-0.2 0.730 120.6 -15.9 -95.5 -34.7 23.2 10.5 37.9 69 84 A R - 0 0 94 -33,-0.2 -3,-2.1 -35,-0.0 2,-0.4 -0.989 46.0-133.3-163.0 172.5 22.7 7.1 36.2 70 85 A N E +F 65 0A 39 -2,-0.3 -5,-0.2 -5,-0.2 -35,-0.1 -0.944 12.5 179.2-135.9 106.3 23.6 4.6 33.4 71 86 A A E - 0 0 21 -7,-2.3 -6,-0.1 -2,-0.4 3,-0.1 0.432 42.5-127.8 -84.9 -1.1 20.8 2.8 31.6 72 87 A H E S+ 0 0 63 -8,-0.3 26,-2.8 1,-0.2 2,-0.3 0.945 73.4 89.4 56.9 50.3 23.4 1.0 29.3 73 88 A S E - G 0 97A 8 24,-0.3 -9,-2.8 101,-0.1 2,-0.3 -0.976 57.1-146.9-166.0 169.8 21.6 2.0 26.1 74 89 A A E -FG 63 96A 0 22,-2.4 22,-2.7 -2,-0.3 2,-0.4 -0.975 7.1-150.4-148.3 153.0 21.3 4.6 23.4 75 90 A T E -FG 62 95A 5 -13,-2.3 -13,-1.9 -2,-0.3 2,-0.4 -0.970 4.7-162.8-127.4 135.7 18.5 6.0 21.2 76 91 A T E -FG 61 94A 0 18,-2.6 18,-2.0 -2,-0.4 2,-0.4 -0.985 6.0-158.1-117.7 132.3 18.7 7.5 17.7 77 92 A W E -FG 60 93A 7 -17,-2.4 -17,-1.7 -2,-0.4 2,-0.4 -0.888 9.0-172.9-104.1 138.6 15.9 9.6 16.3 78 93 A S E +FG 59 92A 0 14,-3.0 14,-2.6 -2,-0.4 2,-0.3 -0.998 31.1 96.0-133.5 130.8 15.6 9.9 12.5 79 94 A G E -FG 58 91A 2 -21,-2.5 -21,-2.5 -2,-0.4 2,-0.4 -0.945 57.0 -78.6-176.9-158.8 13.2 12.3 10.9 80 95 A Q E -FG 57 90A 10 10,-2.1 10,-2.2 -2,-0.3 2,-0.4 -0.993 23.2-129.8-142.6 148.2 12.4 15.6 9.3 81 96 A Y E -FG 56 89A 39 -25,-2.3 -25,-1.8 -2,-0.4 2,-0.5 -0.730 19.5-161.8 -91.1 135.9 11.7 19.1 10.3 82 97 A V E - 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