==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=6-MAY-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 16-OCT-11 3U8A . COMPND 2 MOLECULE: FLUORESCENT PROTEIN RSTAGRFP; . SOURCE 2 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT; . AUTHOR S.PLETNEV . 892 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33148.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 632 70.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 53 5.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 407 45.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 75 8.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 64 7.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 25 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 4 0 1 7 0 4 4 8 4 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A E 0 0 161 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 131.9 9.6 -10.8 -18.2 2 4 A L + 0 0 79 75,-0.1 2,-0.7 78,-0.1 75,-0.0 0.633 360.0 77.3 -76.9 -25.0 6.9 -11.9 -15.6 3 5 A I S S- 0 0 4 74,-0.1 2,-0.3 77,-0.1 4,-0.1 -0.856 71.9-163.0 -95.5 115.6 5.1 -8.6 -15.1 4 6 A K - 0 0 141 -2,-0.7 29,-0.1 2,-0.2 -2,-0.1 -0.791 35.7 -96.5-114.1 135.2 2.8 -8.2 -18.1 5 7 A E S S+ 0 0 124 -2,-0.3 28,-0.4 26,-0.1 2,-0.4 0.571 114.8 35.5 -22.1 -37.7 1.1 -5.0 -19.4 6 8 A N S S- 0 0 82 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.994 76.1-175.6-121.1 133.6 -2.1 -6.0 -17.6 7 9 A M E -A 30 0A 10 23,-2.0 23,-1.7 -2,-0.4 2,-0.2 -0.980 16.5-146.5-131.6 143.3 -2.2 -7.9 -14.1 8 10 A H E -A 29 0A 116 101,-0.5 103,-2.5 -2,-0.3 2,-0.3 -0.675 19.1-162.5 -97.9 154.5 -4.8 -9.4 -11.7 9 11 A M E -Ab 28 111A 3 19,-1.9 19,-2.0 -2,-0.2 2,-0.4 -0.985 12.3-162.7-137.3 152.3 -4.5 -9.5 -7.9 10 12 A K E -Ab 27 112A 107 101,-2.8 103,-3.3 -2,-0.3 2,-0.4 -0.994 16.1-165.2-129.8 136.6 -5.9 -11.3 -5.0 11 13 A L E -Ab 26 113A 1 15,-2.4 15,-1.7 -2,-0.4 2,-0.5 -0.977 16.3-172.9-134.0 134.3 -5.7 -10.0 -1.4 12 14 A Y E -Ab 25 114A 109 101,-2.7 103,-2.7 -2,-0.4 2,-0.4 -0.982 15.9-177.6-116.1 127.5 -6.3 -11.4 2.1 13 15 A M E +Ab 24 115A 4 11,-2.9 11,-2.3 -2,-0.5 2,-0.4 -0.982 7.5 176.2-127.7 137.3 -6.1 -9.0 5.1 14 16 A E E +Ab 23 116A 56 101,-1.8 103,-2.2 -2,-0.4 2,-0.3 -0.998 18.9 137.1-135.3 140.3 -6.4 -9.6 8.7 15 17 A G E -Ab 22 117A 4 7,-2.1 7,-2.1 -2,-0.4 2,-0.4 -0.968 39.8-130.2-164.3-179.7 -6.0 -7.2 11.5 16 18 A T E -Ab 21 118A 33 101,-1.3 103,-2.9 -2,-0.3 2,-0.4 -0.983 16.1-171.8-143.4 131.6 -7.1 -5.6 14.8 17 19 A V E > S-Ab 20 119A 0 3,-2.1 3,-1.9 -2,-0.4 103,-0.2 -0.992 78.7 -18.1-129.2 128.1 -7.6 -1.9 15.4 18 20 A N T 3 S- 0 0 20 101,-2.1 -1,-0.1 -2,-0.4 102,-0.1 0.915 130.8 -49.5 35.4 58.1 -8.2 -0.8 19.0 19 21 A N T 3 S+ 0 0 66 100,-0.4 2,-0.7 1,-0.2 -1,-0.2 0.510 111.3 123.3 71.5 1.0 -9.1 -4.4 20.0 20 22 A H E < -A 17 0A 59 -3,-1.9 -3,-2.1 30,-0.0 2,-0.2 -0.870 48.5-156.4 -98.8 112.2 -11.6 -4.8 17.1 21 23 A H E +A 16 0A 70 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.573 22.4 155.2 -85.2 148.1 -10.6 -7.8 14.9 22 24 A F E -A 15 0A 0 -7,-2.1 -7,-2.1 -2,-0.2 2,-0.3 -0.976 27.8-144.2-160.7 162.0 -11.6 -8.1 11.2 23 25 A K E -AC 14 45A 77 22,-1.7 21,-2.0 -2,-0.3 22,-1.6 -0.985 13.8-171.4-132.8 146.9 -10.7 -9.7 7.9 24 26 A C E -AC 13 43A 1 -11,-2.3 -11,-2.9 -2,-0.3 2,-0.3 -0.961 4.1-167.1-123.6 152.1 -11.0 -8.3 4.4 25 27 A T E -AC 12 42A 47 17,-2.2 17,-2.9 -2,-0.3 2,-0.3 -0.896 11.3-176.8-122.3 166.8 -10.5 -9.9 1.0 26 28 A S E -AC 11 41A 10 -15,-1.7 -15,-2.4 -2,-0.3 2,-0.4 -0.993 14.8-160.7-156.1 160.4 -10.2 -8.1 -2.3 27 29 A E E +AC 10 40A 106 13,-2.0 13,-3.0 -2,-0.3 2,-0.3 -0.988 29.7 130.6-136.6 144.1 -9.9 -8.7 -6.0 28 30 A G E -AC 9 39A 0 -19,-2.0 -19,-1.9 -2,-0.4 2,-0.3 -0.913 32.1-138.4-164.2-162.6 -8.7 -6.4 -8.6 29 31 A E E +AC 8 38A 58 9,-1.8 9,-2.8 -2,-0.3 2,-0.3 -0.961 17.6 167.5-165.3 169.2 -6.5 -5.6 -11.6 30 32 A G E -AC 7 37A 9 -23,-1.7 -23,-2.0 -2,-0.3 7,-0.3 -0.916 42.6 -93.0-167.8-172.4 -4.3 -2.9 -13.0 31 33 A K E >>> - C 0 36A 86 5,-2.7 4,-2.2 -2,-0.3 5,-1.4 -0.886 30.2-164.1-126.4 91.5 -1.7 -1.8 -15.6 32 34 A P T 345S+ 0 0 0 0, 0.0 -1,-0.2 0, 0.0 -26,-0.1 0.840 89.8 45.8 -47.5 -38.2 1.6 -2.2 -13.8 33 35 A Y T 345S+ 0 0 91 -28,-0.4 35,-0.2 1,-0.2 -27,-0.1 0.762 111.4 50.6 -87.8 -23.9 3.4 -0.1 -16.4 34 36 A E T <45S- 0 0 105 -3,-0.7 33,-0.2 -29,-0.3 -1,-0.2 0.820 108.1-122.9 -69.9 -35.1 0.8 2.7 -16.5 35 37 A G T <5 + 0 0 1 -4,-2.2 178,-2.2 1,-0.3 2,-0.3 0.671 69.5 124.4 91.6 23.6 0.9 3.0 -12.7 36 38 A T E < +CD 31 212A 37 -5,-1.4 -5,-2.7 176,-0.2 2,-0.3 -0.899 28.5 158.3-122.5 145.8 -2.9 2.3 -12.4 37 39 A Q E -CD 30 211A 10 174,-1.8 174,-2.3 -2,-0.3 2,-0.4 -0.992 22.5-150.4-162.5 159.9 -5.1 -0.2 -10.5 38 40 A T E -CD 29 210A 18 -9,-2.8 -9,-1.8 -2,-0.3 2,-0.4 -0.994 11.4-168.6-134.8 131.6 -8.5 -0.9 -9.0 39 41 A M E -CD 28 209A 5 170,-2.6 170,-2.6 -2,-0.4 2,-0.6 -0.983 11.4-157.7-122.2 136.4 -9.4 -3.0 -6.0 40 42 A R E -CD 27 208A 49 -13,-3.0 -13,-2.0 -2,-0.4 2,-0.4 -0.943 24.4-167.5-110.5 115.8 -12.8 -4.2 -4.9 41 43 A I E -CD 26 207A 0 166,-3.3 166,-2.4 -2,-0.6 2,-0.4 -0.809 12.6-165.5-114.3 138.5 -12.6 -5.0 -1.2 42 44 A K E -CD 25 206A 120 -17,-2.9 -17,-2.2 -2,-0.4 2,-0.8 -0.981 20.4-137.9-120.5 130.2 -15.0 -6.9 1.1 43 45 A V E +C 24 0A 14 162,-2.5 -19,-0.3 -2,-0.4 3,-0.1 -0.805 30.4 173.6 -77.8 114.4 -15.0 -6.8 4.9 44 46 A V E + 0 0 85 -21,-2.0 2,-0.3 -2,-0.8 -20,-0.2 0.569 59.8 30.3-104.8 -9.6 -15.7 -10.5 5.5 45 47 A E E S+C 23 0A 78 -22,-1.6 -22,-1.7 1,-0.1 -1,-0.3 -0.989 118.4 15.6-152.2 140.2 -15.2 -10.7 9.3 46 48 A G S S- 0 0 29 -2,-0.3 -2,-0.1 -24,-0.2 -1,-0.1 0.664 93.2-169.8 69.5 19.4 -15.8 -8.1 12.0 47 49 A G + 0 0 39 2,-0.1 -1,-0.2 -4,-0.1 2,-0.1 -0.556 55.7 46.7 -92.1 160.8 -17.9 -5.9 9.8 48 50 A P S S- 0 0 81 0, 0.0 156,-0.1 0, 0.0 155,-0.1 0.666 89.4-132.7 -68.1 150.7 -19.1 -3.3 9.8 49 51 A L - 0 0 6 154,-0.3 4,-0.1 155,-0.1 -2,-0.1 -0.461 1.9-144.0 -68.8 134.4 -15.9 -1.8 11.1 50 52 A P S S+ 0 0 55 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.053 82.2 59.3 -85.2 23.3 -16.3 0.6 14.1 51 53 A F S S- 0 0 0 79,-0.1 2,-0.3 80,-0.1 78,-0.2 -0.960 102.8 -74.2-142.8 167.5 -13.5 2.8 12.6 52 54 A A > - 0 0 1 76,-2.0 3,-1.8 -2,-0.3 4,-0.2 -0.416 30.4-145.6 -66.2 125.1 -12.8 4.6 9.4 53 55 A F G >> S+ 0 0 0 -2,-0.3 3,-2.9 1,-0.3 4,-0.5 0.836 90.6 80.7 -58.1 -29.8 -12.0 2.3 6.5 54 56 A D G >4 S+ 0 0 6 1,-0.3 3,-1.1 2,-0.2 4,-0.4 0.771 79.0 66.0 -48.8 -32.9 -9.6 5.0 5.3 55 57 A I G <4 S+ 0 0 2 -3,-1.8 -1,-0.3 1,-0.2 -2,-0.2 0.661 102.3 49.0 -68.6 -11.7 -6.9 3.9 7.7 56 58 A L G X4 S+ 0 0 0 -3,-2.9 3,-2.3 -4,-0.2 4,-0.3 0.571 83.9 96.8 -94.4 -10.6 -6.7 0.6 5.8 57 59 A A G X< S+ 0 0 2 -3,-1.1 3,-1.1 -4,-0.5 -2,-0.1 0.799 82.2 47.8 -55.7 -47.3 -6.4 2.2 2.4 58 60 A T G 3 S+ 0 0 28 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.501 101.0 67.2 -69.7 -5.0 -2.6 2.0 2.0 59 61 A S G < 0 0 0 -3,-2.3 -1,-0.2 1,-0.1 -2,-0.2 0.495 360.0 360.0-100.3 -2.1 -2.4 -1.5 3.1 60 62 A F < 0 0 9 -3,-1.1 -2,-0.2 -4,-0.3 -1,-0.1 0.784 360.0 360.0 -93.3 360.0 -4.2 -2.9 0.1 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 66 A S > 0 0 13 0, 0.0 3,-1.5 0, 0.0 -2,-0.0 0.000 360.0 360.0 360.0 74.9 0.3 -2.6 -4.3 63 67 A R G > + 0 0 14 1,-0.3 3,-0.5 2,-0.1 13,-0.2 0.577 360.0 72.4 -77.6 -15.8 3.8 -1.7 -5.2 64 68 A T G 3 S+ 0 0 0 1,-0.2 2,-0.3 12,-0.1 -1,-0.3 0.671 88.9 65.9 -64.2 -21.0 4.0 -3.6 -8.5 65 69 A F G < + 0 0 1 -3,-1.5 2,-0.6 -30,-0.1 148,-0.4 -0.265 68.2 120.8-105.9 45.0 1.7 -1.0 -10.1 66 70 A I < - 0 0 2 -3,-0.5 2,-0.4 -2,-0.3 148,-0.2 -0.946 61.6-131.9-109.5 111.1 4.0 2.1 -10.0 67 71 A N E -e 214 0A 43 146,-2.0 148,-3.4 -2,-0.6 2,-0.7 -0.470 20.5-148.2 -54.2 119.4 4.8 3.6 -13.4 68 72 A H E -e 215 0A 32 -2,-0.4 3,-0.4 146,-0.2 148,-0.1 -0.844 14.2-164.1 -96.9 113.3 8.5 4.1 -13.5 69 73 A T > + 0 0 45 146,-1.7 3,-2.3 -2,-0.7 145,-0.0 -0.425 64.9 18.0 -78.8 170.4 9.5 7.2 -15.6 70 74 A Q T 3 S- 0 0 151 1,-0.3 -1,-0.2 -2,-0.1 146,-0.0 0.735 124.8 -67.9 39.2 39.7 13.0 8.0 -16.9 71 75 A G T 3 S+ 0 0 72 -3,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.518 74.8 166.6 71.0 7.2 14.3 4.5 -16.5 72 76 A I < - 0 0 3 -3,-2.3 -1,-0.2 143,-0.1 143,-0.1 -0.414 49.1-107.7 -54.7 118.6 14.3 4.4 -12.6 73 77 A P - 0 0 33 0, 0.0 2,-1.3 0, 0.0 3,-0.1 -0.249 27.7-122.1 -55.7 129.9 14.8 0.7 -12.0 74 78 A D > - 0 0 19 1,-0.2 4,-2.8 -6,-0.1 5,-0.2 -0.627 24.6-167.9 -80.7 98.1 11.6 -0.8 -10.8 75 79 A F H > S+ 0 0 2 -2,-1.3 4,-0.5 1,-0.2 -1,-0.2 0.856 87.5 42.2 -46.3 -41.9 12.6 -2.3 -7.4 76 80 A W H 4 S+ 0 0 0 -13,-0.2 -1,-0.2 2,-0.2 4,-0.2 0.815 113.4 49.0 -83.6 -34.4 9.3 -4.2 -7.1 77 81 A K H >4 S+ 0 0 9 1,-0.2 3,-1.4 -14,-0.2 -2,-0.2 0.925 107.4 56.2 -72.1 -42.8 9.1 -5.5 -10.8 78 82 A Q H 3< S+ 0 0 56 -4,-2.8 -1,-0.2 1,-0.2 -2,-0.2 0.757 94.1 73.5 -54.9 -26.4 12.7 -6.7 -10.6 79 83 A S T 3X S+ 0 0 0 -4,-0.5 4,-2.8 -5,-0.2 -1,-0.2 0.606 80.3 139.1 -71.7 -8.3 11.8 -8.9 -7.6 80 84 A F T <4 + 0 0 11 -3,-1.4 -78,-0.1 2,-0.3 -77,-0.1 -0.543 50.8 26.2 -86.8 153.5 9.8 -11.5 -9.5 81 85 A P T 4 S+ 0 0 66 0, 0.0 25,-0.5 0, 0.0 -1,-0.3 -0.941 131.7 41.2 -77.9 -23.4 9.3 -14.4 -9.6 82 86 A E T 4 S- 0 0 74 1,-0.1 -2,-0.3 23,-0.1 2,-0.1 0.872 96.5-157.3 -50.1 -44.7 10.3 -14.5 -5.9 83 87 A G < - 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