==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=6-MAY-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 16-OCT-11 3U8C . COMPND 2 MOLECULE: FLUORESCENT PROTEIN RSTAGRFP; . SOURCE 2 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT; . AUTHOR S.PLETNEV . 892 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 32813.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 652 73.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 407 45.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 81 9.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 77 8.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 24 2.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 11 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 4 0 0 8 0 4 5 7 4 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A E 0 0 206 0, 0.0 77,-0.1 0, 0.0 76,-0.0 0.000 360.0 360.0 360.0 -41.2 9.0 -9.9 -17.6 2 4 A L + 0 0 73 75,-0.2 2,-0.8 2,-0.1 75,-0.0 0.401 360.0 84.9 -95.8 -7.8 6.6 -11.9 -15.5 3 5 A I S S- 0 0 5 77,-0.1 2,-0.1 74,-0.1 4,-0.1 -0.888 76.0-152.5 -98.3 111.3 4.6 -8.8 -14.9 4 6 A K - 0 0 132 -2,-0.8 29,-0.1 2,-0.2 -2,-0.1 -0.477 30.3-100.5 -93.2 160.5 2.3 -8.4 -17.8 5 7 A E S S+ 0 0 129 -2,-0.1 28,-0.4 26,-0.1 2,-0.4 0.767 118.8 32.0 -46.6 -31.8 0.8 -5.2 -19.3 6 8 A N S S+ 0 0 79 25,-0.2 2,-0.4 26,-0.1 25,-0.2 -0.997 79.7 179.7-125.3 130.7 -2.3 -6.1 -17.4 7 9 A M E -A 30 0A 9 23,-2.1 23,-2.3 -2,-0.4 2,-0.3 -0.993 16.8-145.6-134.5 139.5 -2.2 -8.1 -14.1 8 10 A H E -A 29 0A 111 101,-0.5 103,-3.1 -2,-0.4 2,-0.4 -0.685 15.9-161.8 -97.3 158.5 -4.8 -9.4 -11.6 9 11 A M E -Ab 28 111A 3 19,-2.6 19,-2.4 -2,-0.3 2,-0.4 -0.996 10.9-162.4-142.8 143.6 -4.3 -9.5 -7.9 10 12 A K E -Ab 27 112A 47 101,-3.0 103,-3.0 -2,-0.4 2,-0.4 -0.963 18.4-163.0-119.5 142.3 -6.0 -11.3 -5.1 11 13 A L E -Ab 26 113A 0 15,-2.7 15,-2.0 -2,-0.4 2,-0.4 -0.984 16.4-167.5-138.8 137.1 -5.5 -10.1 -1.4 12 14 A Y E -Ab 25 114A 109 101,-3.1 103,-3.8 -2,-0.4 2,-0.4 -0.960 15.1-179.1-117.7 129.6 -6.0 -11.4 2.1 13 15 A M E +Ab 24 115A 5 11,-3.0 11,-3.0 -2,-0.4 2,-0.4 -0.996 7.8 177.2-134.6 131.0 -5.9 -9.1 5.1 14 16 A E E +Ab 23 116A 48 101,-2.5 103,-2.1 -2,-0.4 2,-0.3 -1.000 19.5 143.5-129.2 133.1 -6.2 -9.7 8.8 15 17 A G E -Ab 22 117A 3 7,-2.0 7,-2.4 -2,-0.4 2,-0.4 -0.945 38.6-137.0-152.7 178.5 -5.9 -7.2 11.5 16 18 A T E -Ab 21 118A 31 101,-1.4 103,-3.2 -2,-0.3 2,-0.5 -0.978 16.6-172.2-138.3 124.0 -7.0 -5.8 14.8 17 19 A V E > S-Ab 20 119A 0 3,-2.3 3,-2.1 -2,-0.4 103,-0.2 -0.982 76.8 -19.2-117.4 121.6 -7.5 -2.0 15.3 18 20 A N T 3 S- 0 0 17 101,-3.3 -1,-0.2 -2,-0.5 102,-0.1 0.923 130.8 -48.0 51.8 50.8 -8.1 -0.9 19.0 19 21 A N T 3 S+ 0 0 69 100,-0.4 2,-0.7 1,-0.2 -1,-0.3 0.415 111.7 122.5 71.9 -2.2 -9.1 -4.4 20.0 20 22 A H E < -A 17 0A 59 -3,-2.1 -3,-2.3 30,-0.0 2,-0.3 -0.824 49.6-153.1 -94.1 120.0 -11.5 -4.9 17.1 21 23 A H E +A 16 0A 63 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.647 23.7 157.3 -86.4 144.1 -10.6 -7.9 14.9 22 24 A F E -A 15 0A 0 -7,-2.4 -7,-2.0 -2,-0.3 2,-0.3 -0.978 27.4-144.9-153.8 169.4 -11.5 -8.1 11.3 23 25 A K E -AC 14 45A 79 22,-1.6 21,-2.0 -2,-0.3 22,-1.5 -0.991 13.6-171.6-135.5 141.3 -10.6 -9.8 8.0 24 26 A C E -AC 13 43A 2 -11,-3.0 -11,-3.0 -2,-0.3 2,-0.3 -0.973 4.5-173.6-124.3 149.5 -10.7 -8.4 4.4 25 27 A T E +AC 12 42A 38 17,-1.9 17,-2.7 -2,-0.4 2,-0.3 -0.942 12.2 174.3-126.0 161.9 -10.3 -10.0 1.1 26 28 A S E -AC 11 41A 3 -15,-2.0 -15,-2.7 -2,-0.3 2,-0.3 -0.987 14.2-164.8-154.1 163.4 -10.1 -8.2 -2.2 27 29 A E E +AC 10 40A 90 13,-1.9 13,-2.7 -2,-0.3 2,-0.3 -0.982 26.6 122.0-140.1 152.5 -9.5 -8.7 -5.9 28 30 A G E -AC 9 39A 1 -19,-2.4 -19,-2.6 -2,-0.3 2,-0.3 -0.956 34.6-129.0-169.5-150.5 -8.7 -6.5 -8.8 29 31 A E E +AC 8 38A 54 9,-1.1 9,-2.3 -2,-0.3 2,-0.3 -0.982 17.5 174.4-164.4 172.3 -6.3 -5.7 -11.5 30 32 A G E -AC 7 37A 7 -23,-2.3 -23,-2.1 -2,-0.3 7,-0.3 -0.882 40.9 -88.7-162.6-166.6 -4.3 -2.9 -13.1 31 33 A K E >>> - C 0 36A 88 5,-2.1 4,-1.7 -2,-0.3 5,-1.5 -0.914 32.2-162.7-126.2 95.6 -1.8 -1.9 -15.6 32 34 A P T 345S+ 0 0 0 0, 0.0 -1,-0.1 0, 0.0 -27,-0.1 0.724 87.7 46.8 -52.5 -35.0 1.6 -2.2 -13.9 33 35 A Y T 345S+ 0 0 106 -28,-0.4 35,-0.1 1,-0.2 -28,-0.1 0.685 111.9 50.3 -92.5 -16.6 3.5 0.0 -16.4 34 36 A E T <45S- 0 0 102 -3,-0.7 33,-0.2 -29,-0.3 -1,-0.2 0.775 108.0-125.2 -74.9 -33.3 0.8 2.8 -16.4 35 37 A G T <5 + 0 0 0 -4,-1.7 178,-2.3 1,-0.3 2,-0.3 0.754 67.5 124.9 87.7 28.0 0.8 2.9 -12.5 36 38 A T E < +CD 31 212A 39 -5,-1.5 -5,-2.1 176,-0.2 -1,-0.3 -0.906 27.0 156.4-122.4 152.2 -2.9 2.3 -12.3 37 39 A Q E -CD 30 211A 11 174,-1.2 174,-2.5 -2,-0.3 2,-0.4 -0.970 24.0-147.2-172.4 152.6 -5.0 -0.3 -10.5 38 40 A T E -CD 29 210A 19 -9,-2.3 -9,-1.1 -2,-0.3 2,-0.4 -0.975 11.7-162.9-130.3 136.8 -8.4 -0.9 -9.0 39 41 A M E -CD 28 209A 6 170,-2.9 170,-2.5 -2,-0.4 2,-0.6 -0.988 9.3-158.5-124.2 137.6 -9.4 -2.9 -6.0 40 42 A R E -CD 27 208A 56 -13,-2.7 -13,-1.9 -2,-0.4 2,-0.4 -0.944 25.4-168.9-108.4 118.6 -12.7 -4.3 -5.0 41 43 A I E -CD 26 207A 0 166,-2.9 166,-2.5 -2,-0.6 2,-0.5 -0.893 15.4-170.1-121.8 139.6 -12.7 -5.0 -1.3 42 44 A K E -CD 25 206A 121 -17,-2.7 -17,-1.9 -2,-0.4 2,-0.8 -0.974 22.2-138.6-126.0 129.9 -15.0 -6.8 1.1 43 45 A V E +C 24 0A 13 162,-3.1 -19,-0.3 -2,-0.5 4,-0.1 -0.793 28.7 174.7 -78.8 112.7 -14.8 -6.8 4.9 44 46 A V E + 0 0 84 -21,-2.0 2,-0.3 -2,-0.8 -20,-0.2 0.518 61.7 31.3-101.9 -6.5 -15.6 -10.6 5.5 45 47 A E E S+C 23 0A 86 -22,-1.5 -22,-1.6 -3,-0.1 -1,-0.2 -0.991 118.6 16.7-151.3 145.1 -15.1 -10.6 9.2 46 48 A G S S- 0 0 25 -2,-0.3 -2,-0.1 -24,-0.2 -1,-0.0 0.630 93.0-169.8 65.7 13.7 -15.7 -8.1 12.0 47 49 A G + 0 0 41 2,-0.1 -1,-0.2 -4,-0.1 2,-0.1 -0.518 57.2 46.4 -80.9 154.9 -17.8 -6.0 9.7 48 50 A P S S- 0 0 78 0, 0.0 156,-0.1 0, 0.0 -1,-0.1 0.661 89.6-133.4 -76.3 148.1 -19.0 -3.4 9.8 49 51 A L - 0 0 3 154,-0.4 4,-0.1 1,-0.1 -2,-0.1 -0.362 2.5-147.5 -61.9 130.3 -15.8 -1.8 11.0 50 52 A P S S+ 0 0 56 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.054 80.5 60.6 -87.2 29.8 -16.2 0.5 13.9 51 53 A F S S- 0 0 0 79,-0.1 2,-0.4 80,-0.1 78,-0.2 -0.956 103.7 -72.8-145.5 165.3 -13.3 2.7 12.6 52 54 A A > - 0 0 1 76,-1.7 3,-1.8 -2,-0.3 4,-0.2 -0.429 30.6-145.6 -65.9 119.6 -12.6 4.6 9.4 53 55 A F G >> S+ 0 0 0 -2,-0.4 3,-3.1 1,-0.2 4,-0.6 0.856 91.2 80.8 -42.2 -43.5 -11.9 2.3 6.5 54 56 A D G >4 S+ 0 0 5 1,-0.3 3,-1.2 2,-0.2 4,-0.4 0.765 80.0 64.3 -33.4 -40.1 -9.5 5.0 5.3 55 57 A I G <4 S+ 0 0 2 -3,-1.8 -1,-0.3 1,-0.3 -2,-0.2 0.645 102.3 49.0 -70.3 -13.9 -6.8 3.8 7.7 56 58 A L G X4 S+ 0 0 0 -3,-3.1 3,-2.4 -4,-0.2 4,-0.5 0.590 85.4 98.1 -90.3 -11.9 -6.5 0.5 5.9 57 59 A A G X< S+ 0 0 2 -3,-1.2 3,-1.0 -4,-0.6 -2,-0.1 0.790 82.0 43.9 -49.9 -46.3 -6.3 2.2 2.4 58 60 A T G 3 S+ 0 0 31 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.413 101.6 70.5 -84.1 -1.7 -2.5 2.0 1.9 59 61 A S G < 0 0 1 -3,-2.4 -1,-0.2 1,-0.1 -2,-0.2 0.520 360.0 360.0 -96.4 -4.3 -2.2 -1.6 3.2 60 62 A F < 0 0 7 -3,-1.0 -2,-0.2 -4,-0.5 -1,-0.1 0.801 360.0 360.0 -88.4 360.0 -4.0 -3.1 0.2 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 66 A S > 0 0 11 0, 0.0 3,-1.2 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 81.3 0.2 -2.6 -4.1 63 67 A R T 3 + 0 0 14 1,-0.3 3,-0.5 2,-0.1 13,-0.2 0.517 360.0 73.5 -80.7 -7.7 3.8 -1.8 -5.2 64 68 A T T 3 S+ 0 0 0 1,-0.2 2,-0.8 12,-0.1 -1,-0.3 0.778 87.6 67.3 -73.2 -26.3 3.9 -3.7 -8.5 65 69 A F < + 0 0 1 -3,-1.2 2,-0.6 -30,-0.1 148,-0.4 -0.280 67.6 122.5 -98.7 48.4 1.7 -1.0 -10.1 66 70 A I - 0 0 2 -2,-0.8 2,-0.3 -3,-0.5 148,-0.2 -0.920 59.3-133.0-111.9 112.5 4.1 2.0 -10.0 67 71 A N E -e 214 0A 43 146,-2.1 148,-3.1 -2,-0.6 2,-0.7 -0.409 19.8-150.4 -57.8 115.7 4.8 3.6 -13.4 68 72 A H E -e 215 0A 30 -2,-0.3 3,-0.4 146,-0.2 2,-0.2 -0.825 15.4-167.0 -92.7 115.3 8.5 4.1 -13.5 69 73 A T > + 0 0 45 146,-1.6 3,-2.2 -2,-0.7 145,-0.0 -0.499 63.6 18.4 -86.7 168.0 9.4 7.2 -15.6 70 74 A Q T 3 S- 0 0 146 1,-0.3 -1,-0.2 -2,-0.2 146,-0.0 0.742 124.9 -69.5 47.0 31.2 12.8 8.1 -16.9 71 75 A G T 3 S+ 0 0 65 -3,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.591 73.6 167.0 68.4 11.3 14.2 4.7 -16.4 72 76 A I < - 0 0 5 -3,-2.2 -1,-0.2 143,-0.2 143,-0.1 -0.426 49.2-104.5 -53.9 127.2 14.3 4.5 -12.6 73 77 A P - 0 0 34 0, 0.0 2,-1.2 0, 0.0 3,-0.1 -0.345 29.7-125.7 -59.0 133.0 14.9 0.8 -11.8 74 78 A D > - 0 0 20 1,-0.2 4,-2.7 -6,-0.1 5,-0.2 -0.703 23.0-169.8 -94.2 92.2 11.7 -0.8 -10.7 75 79 A F T 4 S+ 0 0 2 -2,-1.2 4,-0.2 1,-0.2 -1,-0.2 0.777 85.3 43.0 -45.4 -38.1 12.6 -2.3 -7.3 76 80 A W T >4 S+ 0 0 0 -13,-0.2 3,-0.8 2,-0.2 -1,-0.2 0.873 111.7 48.5 -86.3 -39.3 9.3 -4.2 -7.2 77 81 A K G >4 S+ 0 0 23 1,-0.2 3,-1.3 -14,-0.2 -75,-0.2 0.915 109.4 56.3 -68.8 -38.3 9.0 -5.5 -10.7 78 82 A Q G 3< S+ 0 0 57 -4,-2.7 -1,-0.2 1,-0.2 -2,-0.2 0.586 92.7 71.8 -61.8 -17.8 12.6 -6.8 -10.6 79 83 A S G <> S+ 0 0 0 -3,-0.8 4,-2.7 -5,-0.2 99,-0.3 0.587 82.4 140.1 -82.5 -7.7 11.8 -8.9 -7.5 80 84 A F T <4 + 0 0 10 -3,-1.3 -77,-0.1 1,-0.3 -78,-0.0 -0.542 50.1 26.8 -81.4 152.1 9.7 -11.4 -9.4 81 85 A P T 4 S+ 0 0 66 0, 0.0 25,-0.6 0, 0.0 -1,-0.3 -0.983 132.0 41.0 -83.8 -15.1 9.3 -14.3 -9.5 82 86 A E T 4 S- 0 0 74 -3,-0.3 -2,-0.3 1,-0.1 24,-0.1 0.895 96.9-161.0 -55.8 -39.5 10.2 -14.4 -5.8 83 87 A G < - 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