==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=22-APR-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNE SYSTEM 10-JAN-12 3VG0 . COMPND 2 MOLECULE: MONOCLONAL ANTIBODY 9F8 FAB FRAGMENT L CHAIN; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR B.CARPENTER,G.R.HEMSWORTH,R.J.ROSS,P.J.ARTYMIUK . 432 2 4 4 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19074.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 282 65.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 9 2.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 172 39.8 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 45 10.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 33 7.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 13 3.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 4 7 1 5 9 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 5 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 L E 0 0 138 0, 0.0 2,-0.2 0, 0.0 95,-0.1 0.000 360.0 360.0 360.0 160.0 77.7 6.2 18.5 2 4 L I - 0 0 18 93,-0.3 2,-0.4 92,-0.2 88,-0.1 -0.336 360.0-156.3 -72.6 126.7 75.7 4.1 16.0 3 5 L V - 0 0 85 -2,-0.2 23,-1.6 94,-0.1 2,-0.6 -0.836 2.8-155.3-100.0 133.8 72.7 5.6 14.2 4 6 L M E -A 25 0A 8 -2,-0.4 2,-0.5 94,-0.4 96,-0.4 -0.961 15.6-161.9-106.4 114.6 69.9 3.3 12.8 5 7 L T E -A 24 0A 66 19,-1.4 19,-2.4 -2,-0.6 2,-0.6 -0.843 16.2-173.9 -98.9 130.3 68.2 5.1 10.0 6 8 L Q - 0 0 9 -2,-0.5 17,-0.1 17,-0.2 94,-0.1 -0.931 8.2-169.7-117.6 101.9 64.8 4.1 8.8 7 9 L S + 0 0 110 -2,-0.6 2,-0.7 15,-0.1 -1,-0.1 0.749 68.0 72.3 -71.8 -27.1 64.3 6.3 5.8 8 10 L P - 0 0 41 0, 0.0 3,-0.1 0, 0.0 -1,-0.1 -0.824 56.6-177.9 -86.4 117.6 60.7 5.4 5.2 9 11 L K S S+ 0 0 59 -2,-0.7 94,-2.1 1,-0.3 2,-0.4 0.820 78.0 23.9 -78.1 -30.5 58.7 7.1 8.0 10 12 L F E S-d 103 0B 139 92,-0.3 2,-0.4 75,-0.0 -1,-0.3 -0.998 71.4-162.5-140.2 133.1 55.4 5.6 6.7 11 13 L M E -d 104 0B 51 92,-2.4 94,-3.8 -2,-0.4 2,-0.3 -0.981 9.4-151.9-127.4 127.4 55.1 2.5 4.6 12 14 L S E +d 105 0B 52 -2,-0.4 2,-0.3 92,-0.2 94,-0.2 -0.779 25.3 145.9-108.9 145.1 51.9 1.7 2.5 13 15 L T E -d 106 0B 4 92,-1.6 94,-3.4 -2,-0.3 2,-0.3 -0.972 36.8-110.3-166.4 167.9 50.6 -1.7 1.5 14 16 L S E > -d 107 0B 42 -2,-0.3 3,-2.3 92,-0.2 64,-0.3 -0.832 47.0 -84.3-114.2 151.3 47.5 -4.0 0.9 15 17 L I T 3 S+ 0 0 96 92,-0.6 64,-0.2 -2,-0.3 62,-0.1 -0.238 118.0 15.7 -48.5 130.4 46.1 -7.0 2.8 16 18 L G T 3 S+ 0 0 37 62,-3.3 61,-0.4 1,-0.2 -1,-0.3 0.518 100.8 120.7 79.7 3.5 48.0 -10.1 1.7 17 19 L D < - 0 0 74 -3,-2.3 61,-2.1 59,-0.1 2,-0.4 -0.221 64.9-109.1 -83.9-178.1 50.9 -8.2 0.0 18 20 L R - 0 0 164 58,-0.2 2,-0.4 59,-0.2 58,-0.2 -0.934 34.0-174.8-113.2 139.1 54.6 -8.3 0.9 19 21 L V E - B 0 75A 7 56,-1.9 56,-3.5 -2,-0.4 2,-0.5 -0.995 12.4-158.5-135.1 139.9 56.3 -5.4 2.6 20 22 L N E - B 0 74A 71 -2,-0.4 2,-0.5 54,-0.2 54,-0.2 -0.984 3.5-166.4-113.8 121.8 60.0 -4.8 3.4 21 23 L I E - B 0 73A 0 52,-2.7 52,-2.9 -2,-0.5 2,-0.3 -0.952 20.1-153.5-103.0 130.0 60.8 -2.3 6.1 22 24 L T E - B 0 72A 62 -2,-0.5 2,-0.4 50,-0.2 50,-0.2 -0.733 16.8-166.1-113.8 156.3 64.5 -1.5 6.0 23 25 L a E - B 0 71A 0 48,-2.5 48,-2.3 -2,-0.3 2,-0.7 -0.943 6.6-161.5-145.3 114.1 67.0 -0.4 8.5 24 26 L K E -AB 5 70A 38 -19,-2.4 -19,-1.4 -2,-0.4 46,-0.3 -0.857 12.7-147.3-108.4 101.6 70.4 1.0 7.5 25 27 L A E -A 4 0A 11 44,-3.2 -21,-0.2 -2,-0.7 -23,-0.0 -0.276 13.5-137.2 -63.6 146.8 73.2 1.0 10.2 26 28 L T S S- 0 0 72 -23,-1.6 2,-0.3 -2,-0.0 -1,-0.1 0.476 84.5 -4.3 -80.8 -6.6 75.9 3.7 10.4 27 29 L Q S S+ 0 0 44 41,-0.2 3,-0.1 -24,-0.2 -2,-0.1 -0.913 74.2 120.9-162.2 179.6 78.6 1.0 11.2 28 30 L N - 0 0 35 -2,-0.3 -3,-0.0 1,-0.1 39,-0.0 0.223 50.4-147.4 122.3 136.5 79.1 -2.7 12.0 29 31 L V S S+ 0 0 124 40,-0.0 2,-0.3 39,-0.0 -1,-0.1 -0.420 78.8 137.2 -96.5 43.5 80.6 -6.1 11.5 30 32 L R + 0 0 5 38,-0.4 38,-1.8 -3,-0.1 62,-0.0 -0.740 56.8 169.0-113.3 141.2 77.1 -7.0 12.9 31 33 L T + 0 0 71 -2,-0.3 2,-1.4 36,-0.2 21,-0.2 -0.220 60.5 123.4-108.5 30.7 74.1 -9.3 12.7 32 34 L A + 0 0 9 18,-0.2 59,-3.0 36,-0.1 2,-0.4 -0.352 38.7 123.8 -87.5 52.1 73.4 -7.6 16.1 33 35 L V E -E 90 0B 4 -2,-1.4 18,-2.9 57,-0.2 2,-0.3 -0.963 39.7-170.1-124.2 130.5 70.0 -6.4 15.0 34 36 L T E -E 89 0B 0 55,-2.3 55,-2.4 -2,-0.4 2,-0.4 -0.891 11.1-147.6-115.2 147.0 66.6 -7.0 16.6 35 37 L W E -EF 88 48B 0 13,-2.8 12,-3.5 -2,-0.3 13,-0.9 -0.962 15.5-172.3-118.8 137.0 63.2 -6.1 15.0 36 38 L Y E -EF 87 46B 0 51,-2.4 51,-1.6 -2,-0.4 2,-0.4 -0.880 17.5-148.4-127.0 154.4 60.1 -5.1 17.0 37 39 L Q E -EF 86 45B 19 8,-2.0 8,-1.9 -2,-0.3 2,-0.5 -0.987 12.7-172.6-116.3 128.4 56.4 -4.4 16.3 38 40 L Q E -E 85 0B 19 47,-2.6 47,-3.0 -2,-0.4 5,-0.1 -0.960 9.9-157.3-121.3 109.9 54.7 -1.7 18.4 39 41 L K > - 0 0 61 -2,-0.5 3,-1.8 4,-0.4 45,-0.2 -0.517 47.1 -67.8 -78.1 153.8 50.9 -1.6 17.8 40 42 L P T 3 S- 0 0 58 0, 0.0 -1,-0.2 0, 0.0 3,-0.1 -0.168 115.9 -1.3 -48.7 126.5 49.2 1.8 18.8 41 43 L G T 3 S+ 0 0 92 1,-0.2 2,-0.3 -3,-0.1 -2,-0.0 0.639 116.0 86.0 69.2 16.6 49.2 2.5 22.5 42 44 L Q S < S- 0 0 101 -3,-1.8 -1,-0.2 0, 0.0 281,-0.1 -0.827 79.8 -77.0-135.6-172.6 50.9 -0.7 23.6 43 45 L S - 0 0 23 -2,-0.3 -4,-0.4 1,-0.1 280,-0.1 -0.513 47.8 -95.0 -94.4 151.1 54.3 -2.2 24.1 44 46 L P - 0 0 1 0, 0.0 277,-0.5 0, 0.0 2,-0.4 -0.115 29.9-153.4 -61.9 151.4 56.9 -3.5 21.6 45 47 L Q E -FG 37 320B 87 -8,-1.9 -8,-2.0 275,-0.1 275,-0.1 -1.000 19.6-120.9-130.5 124.5 57.1 -7.2 20.7 46 48 L A E +F 36 0B 3 273,-0.7 -10,-0.3 -2,-0.4 3,-0.1 -0.406 35.2 168.3 -59.9 133.6 60.3 -8.8 19.4 47 49 L L E + 0 0 6 -12,-3.5 8,-3.0 1,-0.4 2,-0.4 0.717 65.2 19.6-108.0 -55.5 59.8 -10.3 16.0 48 50 L I E -FH 35 54B 1 -13,-0.9 -13,-2.8 6,-0.2 -1,-0.4 -0.980 64.7-166.9-127.8 125.5 63.2 -11.1 14.7 49 51 L F E > S+ H 0 53B 58 4,-2.5 4,-1.4 -2,-0.4 3,-0.4 -0.882 71.4 14.8-115.7 149.2 66.4 -11.6 16.7 50 52 L L T 4 S- 0 0 35 -2,-0.3 2,-2.3 1,-0.2 -1,-0.2 0.915 122.3 -74.4 51.4 51.3 70.1 -11.7 15.5 51 53 L A T 4 S+ 0 0 3 -18,-2.9 -1,-0.2 -3,-0.3 21,-0.2 -0.197 129.7 30.3 67.3 -45.4 69.0 -10.3 12.0 52 54 L S T 4 S+ 0 0 65 -2,-2.3 2,-0.4 -3,-0.4 -1,-0.2 -0.025 89.9 103.1-141.4 28.0 67.4 -13.6 10.7 53 55 L N E < -H 49 0B 73 -4,-1.4 -4,-2.5 10,-0.1 2,-0.5 -0.961 62.5-127.5-117.1 140.0 65.9 -15.6 13.7 54 56 L R E -H 48 0B 115 -2,-0.4 -6,-0.2 -6,-0.2 3,-0.1 -0.717 18.7-128.6 -92.4 120.9 62.2 -15.7 14.6 55 57 L H > - 0 0 20 -8,-3.0 3,-1.8 -2,-0.5 -1,-0.0 -0.359 46.1 -83.3 -58.7 140.1 61.0 -15.0 18.2 56 58 L T T 3 S+ 0 0 122 1,-0.2 -1,-0.1 -2,-0.0 0, 0.0 -0.126 114.8 16.2 -43.7 139.6 58.7 -17.7 19.6 57 59 L G T 3 S+ 0 0 81 1,-0.3 -1,-0.2 -3,-0.1 -2,-0.1 0.535 88.8 131.2 70.7 6.9 55.0 -17.4 18.7 58 60 L V < - 0 0 20 -3,-1.8 -1,-0.3 -11,-0.1 -11,-0.0 -0.829 66.6-110.9 -85.7 124.0 55.8 -15.0 15.8 59 61 L P > - 0 0 64 0, 0.0 3,-1.8 0, 0.0 -1,-0.0 -0.245 25.4-108.9 -65.6 151.1 53.9 -16.3 12.7 60 62 L A T 3 S+ 0 0 74 1,-0.3 -2,-0.0 -6,-0.0 16,-0.0 0.596 108.2 73.4 -53.4 -21.0 56.1 -17.8 9.9 61 63 L R T 3 S+ 0 0 36 15,-0.1 15,-2.7 16,-0.1 2,-0.6 0.514 78.6 84.9 -77.0 -7.9 55.4 -14.9 7.4 62 64 L F E < +C 75 0A 6 -3,-1.8 2,-0.3 13,-0.2 13,-0.2 -0.886 60.2 166.3 -91.2 122.0 57.7 -12.5 9.4 63 65 L T E -C 74 0A 59 11,-2.2 11,-2.8 -2,-0.6 2,-0.3 -0.951 21.4-152.0-134.4 156.2 61.4 -13.0 8.2 64 66 L G E +C 73 0A 12 -2,-0.3 2,-0.3 9,-0.2 9,-0.2 -0.919 16.7 169.0-126.2 153.7 64.6 -11.0 8.7 65 67 L S E +C 72 0A 58 7,-1.3 7,-2.7 -2,-0.3 2,-0.1 -0.989 19.3 80.4-157.9 155.6 67.7 -10.8 6.6 66 68 L G E -C 71 0A 36 -2,-0.3 2,-0.3 5,-0.2 5,-0.2 -0.334 40.1-128.6 128.9 159.7 70.9 -8.8 6.2 67 69 L S E > -C 70 0A 89 3,-0.5 2,-1.6 -2,-0.1 3,-0.7 -0.979 66.2 -11.9-138.0 148.3 74.5 -8.2 7.3 68 70 L G T 3 S- 0 0 18 -38,-1.8 -38,-0.4 -2,-0.3 -41,-0.2 -0.449 127.7 -3.3 77.0 -86.1 76.7 -5.3 8.4 69 71 L T T 3 S+ 0 0 64 -2,-1.6 -44,-3.2 1,-0.2 2,-0.4 0.823 118.1 60.8-109.2 -53.5 74.8 -2.2 7.6 70 72 L D E < +BC 24 67A 100 -3,-0.7 -3,-0.5 -46,-0.3 2,-0.3 -0.679 56.8 179.6 -92.6 130.1 71.5 -2.9 5.8 71 73 L F E -BC 23 66A 2 -48,-2.3 -48,-2.5 -2,-0.4 2,-0.5 -0.961 9.8-156.6-130.2 144.3 68.7 -5.0 7.4 72 74 L T E -BC 22 65A 32 -7,-2.7 -7,-1.3 -2,-0.3 2,-0.7 -0.971 9.1-162.3-131.6 120.4 65.3 -6.0 6.1 73 75 L L E -BC 21 64A 0 -52,-2.9 -52,-2.7 -2,-0.5 2,-0.5 -0.913 20.3-161.5 -93.1 119.4 62.2 -7.0 7.9 74 76 L T E -BC 20 63A 17 -11,-2.8 -11,-2.2 -2,-0.7 2,-0.6 -0.909 9.6-167.8-103.3 127.6 59.9 -8.8 5.4 75 77 L I E -BC 19 62A 0 -56,-3.5 -56,-1.9 -2,-0.5 3,-0.3 -0.951 10.9-166.9-110.9 106.0 56.2 -9.2 6.1 76 78 L N + 0 0 56 -15,-2.7 -58,-0.2 -2,-0.6 -59,-0.1 -0.641 69.1 13.1 -82.7 151.9 54.6 -11.7 3.7 77 79 L N S S- 0 0 82 -61,-0.4 -1,-0.2 -2,-0.2 -59,-0.2 0.926 90.8-140.3 51.7 60.7 50.8 -11.9 3.4 78 80 L V - 0 0 0 -61,-2.1 -62,-3.3 -3,-0.3 2,-0.3 -0.209 18.6-161.4 -57.0 136.1 50.1 -8.7 5.3 79 81 L K > - 0 0 89 -64,-0.2 3,-1.8 -3,-0.1 4,-0.2 -0.778 31.8-111.2-116.4 167.3 47.1 -8.9 7.7 80 82 L S G > S+ 0 0 16 1,-0.3 3,-1.8 -2,-0.3 89,-0.1 0.839 115.6 65.4 -71.0 -24.6 45.2 -6.0 9.3 81 83 L E G 3 S+ 0 0 85 1,-0.3 -1,-0.3 88,-0.1 87,-0.1 0.625 86.0 75.1 -67.5 -14.1 46.6 -6.9 12.7 82 84 L D G < + 0 0 3 -3,-1.8 2,-0.3 86,-0.1 -1,-0.3 0.548 60.4 122.6 -80.3 -8.0 50.0 -5.9 11.3 83 85 L L < + 0 0 10 -3,-1.8 2,-0.3 -4,-0.2 22,-0.1 -0.425 47.9 106.3 -48.6 112.5 49.0 -2.3 11.6 84 86 L A S S- 0 0 3 20,-0.3 20,-2.5 -2,-0.3 2,-0.5 -0.969 76.3 -87.8-174.8 172.5 51.9 -1.1 13.8 85 87 L D E -EI 38 103B 50 -47,-3.0 -47,-2.6 -2,-0.3 2,-0.4 -0.882 40.5-156.6 -97.2 130.2 55.2 0.8 14.0 86 88 L Y E +EI 37 102B 0 16,-2.8 16,-2.3 -2,-0.5 2,-0.3 -0.940 19.6 165.4-111.7 129.3 58.2 -1.5 13.1 87 89 L F E -E 36 0B 3 -51,-1.6 -51,-2.4 -2,-0.4 2,-0.3 -0.981 26.5-131.8-142.3 155.0 61.7 -0.6 14.3 88 90 L a E -E 35 0B 0 -2,-0.3 11,-2.7 -53,-0.2 2,-0.4 -0.759 10.4-150.0-102.4 150.9 65.2 -2.1 14.7 89 91 L L E -EJ 34 98B 1 -55,-2.4 -55,-2.3 -2,-0.3 2,-0.6 -0.989 9.4-146.2-115.4 132.2 67.6 -2.1 17.7 90 92 L Q E +EJ 33 97B 4 7,-3.1 7,-1.3 -2,-0.4 -57,-0.2 -0.875 22.3 171.1 -89.4 120.7 71.3 -2.2 17.1 91 93 L H + 0 0 30 -59,-3.0 -58,-0.1 -2,-0.6 5,-0.1 0.015 49.7 96.8-121.3 31.0 73.0 -4.1 19.9 92 94 L W S S+ 0 0 146 -60,-0.3 2,-0.3 1,-0.2 4,-0.1 0.913 92.9 12.8 -76.3 -48.2 76.6 -4.4 18.6 93 95 L N S S- 0 0 38 2,-0.2 -1,-0.2 -61,-0.1 4,-0.1 -0.849 94.7 -62.5-125.6 160.0 78.0 -1.4 20.6 94 96 L Y S S+ 0 0 92 -2,-0.3 -92,-0.2 2,-0.1 2,-0.1 -0.625 107.2 44.6 -77.2 144.6 77.1 0.9 23.5 95 97 L P S S- 0 0 8 0, 0.0 -93,-0.3 0, 0.0 -2,-0.2 0.707 88.2-132.2 -70.8 152.3 74.8 2.8 23.6 96 98 L L - 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