==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=10-MAR-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE 15-DEC-08 2W5X . COMPND 2 MOLECULE: ALKALINE PHOSPHATASE; . SOURCE 2 ORGANISM_SCIENTIFIC: ANTARCTIC BACTERIUM TAB5; . AUTHOR D.KOUTSIOULIS,A.LYSKOWSKI,S.MAKI,E.GUTHRIE,G.FELLER, . 692 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 23883.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 490 70.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 84 12.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 56 8.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 3 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 43 6.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 90 13.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 165 23.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 11 1.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 5 0 2 5 1 0 6 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 8 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 8 2 2 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 2 2 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 30 A Q 0 0 176 0, 0.0 2,-0.4 0, 0.0 3,-0.3 0.000 360.0 360.0 360.0 174.7 -1.1 42.2 -15.0 2 31 A L - 0 0 150 1,-0.2 2,-0.0 318,-0.0 0, 0.0 -0.951 360.0 -9.7-134.8 114.6 -3.9 40.8 -12.9 3 32 A K + 0 0 98 -2,-0.4 -1,-0.2 338,-0.1 0, 0.0 0.624 40.0 166.9-153.5 158.1 -3.0 39.2 -10.7 4 33 A T - 0 0 34 -3,-0.3 2,-0.2 338,-0.1 338,-0.2 -0.878 28.1-149.5-107.4 110.7 -0.2 37.5 -8.9 5 34 A P - 0 0 2 0, 0.0 260,-0.2 0, 0.0 259,-0.1 -0.568 4.9-158.6 -74.1 140.3 -0.8 36.3 -5.3 6 35 A K S S+ 0 0 63 258,-1.8 2,-0.3 -2,-0.2 259,-0.2 0.850 78.9 32.5 -78.9 -40.1 2.2 36.2 -3.0 7 36 A N E -a 265 0A 5 257,-2.2 259,-2.8 212,-0.2 2,-0.4 -0.828 67.2-155.8-119.3 155.2 0.6 33.7 -0.6 8 37 A V E -ab 266 221A 0 212,-2.3 214,-2.2 -2,-0.3 2,-0.5 -0.997 5.0-170.9-132.8 132.2 -1.9 30.8 -1.0 9 38 A I E -ab 267 222A 0 257,-2.8 259,-2.8 -2,-0.4 2,-0.6 -0.980 8.2-167.7-118.7 117.9 -4.2 29.2 1.5 10 39 A L E -ab 268 223A 1 212,-3.4 214,-2.1 -2,-0.5 2,-0.5 -0.922 2.7-167.4-107.6 115.7 -5.9 26.0 0.3 11 40 A L E -ab 269 224A 0 257,-3.2 259,-2.3 -2,-0.6 2,-0.6 -0.909 3.5-164.5-105.8 123.0 -8.8 24.9 2.5 12 41 A I E -ab 270 225A 0 212,-2.5 214,-2.5 -2,-0.5 2,-0.9 -0.925 10.5-162.4-113.9 118.3 -10.1 21.3 1.9 13 42 A S E > - b 0 226A 1 257,-2.4 3,-1.6 -2,-0.6 259,-0.4 -0.847 26.3-136.9 -93.5 103.2 -13.4 20.2 3.3 14 43 A D T 3 S- 0 0 1 212,-1.6 216,-0.6 -2,-0.9 217,-0.1 -0.453 80.4 -0.5 -67.4 120.9 -13.1 16.4 3.1 15 44 A G T 3 S+ 0 0 1 258,-0.5 260,-1.5 -2,-0.3 2,-0.8 0.555 91.6 140.5 85.3 10.3 -16.3 14.8 1.8 16 45 A A < + 0 0 0 -3,-1.6 -1,-0.2 258,-0.2 -3,-0.1 -0.770 23.2 176.5 -97.1 105.3 -18.3 18.0 1.3 17 46 A G > - 0 0 1 -2,-0.8 4,-2.4 258,-0.2 5,-0.2 -0.277 54.5 -83.4 -84.4-176.9 -20.4 18.2 -1.8 18 47 A L H > S+ 0 0 6 294,-0.4 4,-2.6 1,-0.2 641,-0.4 0.836 130.7 56.5 -60.3 -33.0 -22.7 21.1 -2.5 19 48 A S H > S+ 0 0 19 2,-0.2 4,-1.2 1,-0.2 -1,-0.2 0.929 107.6 47.3 -64.2 -43.8 -25.4 19.6 -0.3 20 49 A Q H >> S+ 0 0 0 2,-0.2 3,-0.7 1,-0.2 4,-0.6 0.929 112.6 49.5 -61.4 -45.6 -23.0 19.5 2.6 21 50 A I H >< S+ 0 0 1 -4,-2.4 3,-1.4 1,-0.2 4,-0.4 0.928 105.4 59.6 -56.1 -43.8 -22.0 23.1 1.9 22 51 A S H >< S+ 0 0 0 -4,-2.6 3,-1.8 1,-0.3 4,-0.4 0.776 88.4 71.7 -59.8 -29.8 -25.7 24.0 1.8 23 52 A S H XX S+ 0 0 0 -4,-1.2 4,-3.0 -3,-0.7 3,-1.9 0.872 87.9 64.1 -56.4 -35.6 -26.3 22.8 5.4 24 53 A T H - 0 0 7 0, 0.0 3,-1.4 0, 0.0 -1,-0.1 -0.301 15.8-126.7 -61.0 145.0 -21.9 31.4 6.0 33 62 A N G > S+ 0 0 37 1,-0.3 3,-2.4 216,-0.2 4,-0.3 0.746 103.2 82.5 -65.1 -21.8 -18.5 32.7 5.1 34 63 A Y G > S+ 0 0 0 1,-0.3 3,-1.9 2,-0.2 -1,-0.3 0.850 78.3 68.2 -43.2 -39.4 -19.1 30.8 1.8 35 64 A T G < S+ 0 0 40 -3,-1.4 640,-0.3 1,-0.3 -1,-0.3 0.558 82.5 73.4 -66.0 -7.3 -21.1 33.9 0.7 36 65 A Q G < S+ 0 0 51 -3,-2.4 -1,-0.3 -4,-0.1 2,-0.2 0.700 80.5 92.7 -77.4 -18.6 -18.0 36.0 0.5 37 66 A F < + 0 0 0 -3,-1.9 638,-0.5 -4,-0.3 281,-0.1 -0.534 39.6 167.9 -81.8 138.8 -16.9 34.2 -2.7 38 67 A K + 0 0 116 279,-0.5 636,-1.8 -2,-0.2 2,-0.5 0.715 64.3 70.7-107.9 -49.0 -17.7 35.5 -6.1 39 68 A N E +C 317 0A 22 278,-0.7 278,-2.2 634,-0.2 2,-0.4 -0.602 64.8 169.8 -76.9 123.0 -15.4 33.3 -8.3 40 69 A I E +C 316 0A 1 -2,-0.5 287,-0.8 347,-0.3 347,-0.4 -0.993 7.5 169.2-138.4 137.6 -16.7 29.7 -8.4 41 70 A G E -Cd 315 327A 0 274,-2.5 274,-2.8 -2,-0.4 2,-0.4 -0.759 24.3-123.9-135.8-174.1 -15.8 26.7 -10.4 42 71 A L E -Cd 314 328A 3 285,-2.4 287,-2.4 272,-0.3 2,-0.4 -1.000 15.8-155.5-140.7 137.1 -16.4 23.0 -10.5 43 72 A I E -Cd 313 329A 0 270,-2.5 270,-2.3 -2,-0.4 2,-0.5 -0.911 14.6-136.6-117.5 141.8 -14.1 20.0 -10.5 44 73 A K E - d 0 330A 47 285,-1.9 287,-2.4 -2,-0.4 288,-0.2 -0.832 19.9-165.1 -89.8 128.1 -14.5 16.4 -11.7 45 74 A T + 0 0 0 -2,-0.5 -1,-0.1 266,-0.3 16,-0.1 0.407 36.0 131.2-104.9 1.5 -13.0 14.1 -9.1 46 75 A S - 0 0 10 264,-0.2 5,-0.2 6,-0.1 285,-0.1 -0.200 50.8-130.6 -57.1 144.5 -12.6 10.7 -10.7 47 76 A S - 0 0 12 3,-2.2 22,-0.2 20,-0.1 20,-0.1 -0.155 33.5 -88.4 -84.6-174.2 -9.1 9.1 -10.2 48 77 A S S S+ 0 0 48 20,-1.9 21,-0.2 1,-0.2 3,-0.1 0.712 125.7 16.6 -68.2 -19.3 -6.9 7.6 -12.9 49 78 A R S S+ 0 0 148 19,-0.5 2,-0.4 1,-0.2 -1,-0.2 0.509 119.6 60.3-128.7 -13.3 -8.6 4.2 -12.5 50 79 A E - 0 0 35 1,-0.1 -3,-2.2 4,-0.0 594,-0.2 -0.977 57.5-149.4-131.9 131.8 -11.9 4.8 -10.6 51 80 A D S S+ 0 0 58 593,-1.6 2,-0.6 -2,-0.4 593,-0.2 0.871 99.1 54.0 -59.7 -36.9 -15.0 6.9 -11.3 52 81 A V S S- 0 0 0 591,-2.7 -1,-0.2 -6,-0.1 258,-0.1 -0.899 86.7-150.8-105.0 114.5 -15.3 7.3 -7.6 53 82 A T - 0 0 1 -2,-0.6 2,-0.2 256,-0.2 255,-0.1 -0.317 2.3-141.0 -80.0 159.2 -12.2 8.7 -5.9 54 83 A D > - 0 0 15 -2,-0.1 4,-1.8 -4,-0.1 5,-0.2 -0.632 37.0 -96.1-106.6 178.3 -10.8 8.1 -2.4 55 84 A X H > S+ 0 0 16 -2,-0.2 4,-1.5 1,-0.2 5,-0.1 0.744 117.6 56.9 -67.6 -23.8 -9.2 10.8 -0.3 56 85 A A H > S+ 0 0 0 2,-0.2 4,-1.9 1,-0.2 18,-0.3 0.966 113.6 33.5 -77.1 -54.1 -5.6 9.8 -1.3 57 86 A S H > S+ 0 0 2 1,-0.2 4,-2.0 2,-0.2 -2,-0.2 0.863 117.6 58.4 -67.5 -33.8 -5.8 10.1 -5.1 58 87 A G H X S+ 0 0 0 -4,-1.8 4,-0.8 1,-0.2 -1,-0.2 0.868 112.6 37.7 -62.8 -38.7 -8.2 13.0 -4.6 59 88 A A H X S+ 0 0 0 -4,-1.5 4,-2.8 2,-0.2 -1,-0.2 0.768 111.1 58.7 -85.4 -28.9 -5.7 15.0 -2.6 60 89 A T H X>S+ 0 0 0 -4,-1.9 4,-2.8 12,-0.3 5,-0.6 0.852 100.0 59.7 -68.4 -31.9 -2.7 13.9 -4.6 61 90 A A H X5S+ 0 0 1 -4,-2.0 4,-1.2 3,-0.2 274,-0.5 0.918 111.7 39.6 -56.7 -45.3 -4.5 15.5 -7.6 62 91 A F H <5S+ 0 0 2 -4,-0.8 -2,-0.2 1,-0.2 -1,-0.2 0.935 122.6 40.1 -68.8 -47.9 -4.4 18.8 -5.8 63 92 A S H <5S+ 0 0 0 -4,-2.8 22,-2.3 1,-0.2 23,-0.2 0.758 131.8 21.5 -78.4 -27.0 -0.9 18.5 -4.3 64 93 A C H <5S- 0 0 7 -4,-2.8 -3,-0.2 20,-0.2 -1,-0.2 0.491 94.3-125.9-124.4 -10.2 0.9 16.9 -7.3 65 94 A G S < - 0 0 30 -2,-0.3 3,-2.4 7,-0.2 6,-0.1 -0.437 56.7 -90.3 -77.8 144.9 -4.9 5.5 -8.3 70 99 A N T 3 S+ 0 0 24 1,-0.3 -1,-0.1 -2,-0.1 3,-0.1 -0.325 116.5 26.6 -49.4 129.5 -7.1 5.4 -5.2 71 100 A A T 3 S+ 0 0 16 1,-0.3 48,-1.6 47,-0.1 -1,-0.3 0.134 89.2 132.4 96.6 -14.2 -4.9 5.0 -2.2 72 101 A A < - 0 0 0 -3,-2.4 2,-0.5 46,-0.2 -1,-0.3 -0.438 41.5-157.8 -71.9 141.4 -1.8 6.6 -3.8 73 102 A I B S-J 116 0B 0 43,-2.0 43,-2.2 -2,-0.1 -16,-0.1 -0.925 72.0 -29.9-122.5 105.9 0.0 9.3 -1.8 74 103 A G S S+ 0 0 0 -2,-0.5 8,-1.7 -18,-0.3 2,-0.3 0.785 110.7 111.7 59.9 28.8 2.1 11.7 -3.8 75 104 A V B -K 81 0C 4 6,-0.2 6,-0.2 41,-0.2 -1,-0.2 -0.963 64.9-117.5-130.3 152.6 2.8 9.2 -6.5 76 105 A A > - 0 0 18 4,-3.1 3,-2.1 -2,-0.3 -7,-0.2 -0.085 43.0 -88.1 -76.8 179.9 1.7 8.9 -10.1 77 106 A D T 3 S+ 0 0 61 -10,-0.6 -9,-0.1 1,-0.3 -1,-0.1 0.840 131.4 53.4 -62.2 -33.5 -0.5 6.2 -11.7 78 107 A D T 3 S- 0 0 103 2,-0.1 -1,-0.3 1,-0.0 -10,-0.0 0.351 121.7-112.5 -81.0 6.7 2.7 4.1 -12.4 79 108 A S S < S+ 0 0 48 -3,-2.1 2,-0.2 1,-0.3 -2,-0.1 0.480 71.0 139.1 77.1 8.2 3.5 4.6 -8.7 80 109 A T - 0 0 76 1,-0.1 -4,-3.1 -5,-0.1 -1,-0.3 -0.586 60.4-100.5 -83.2 145.6 6.5 6.8 -9.2 81 110 A A B -K 75 0C 69 -6,-0.2 2,-0.3 -2,-0.2 -6,-0.2 -0.356 39.1-164.7 -64.3 140.1 7.1 9.8 -6.9 82 111 A V - 0 0 35 -8,-1.7 2,-0.2 -2,-0.1 -17,-0.1 -0.958 28.1 -96.4-129.1 149.3 6.1 13.2 -8.2 83 112 A K - 0 0 94 -2,-0.3 2,-0.2 -19,-0.1 -9,-0.0 -0.398 40.1-148.5 -68.0 130.7 6.9 16.6 -7.0 84 113 A S > - 0 0 1 -2,-0.2 4,-2.5 1,-0.1 -20,-0.2 -0.481 27.0-106.8 -93.3 170.0 4.3 18.2 -4.8 85 114 A I H > S+ 0 0 2 -22,-2.3 4,-2.7 1,-0.2 5,-0.1 0.810 120.5 57.0 -64.1 -30.5 3.3 21.9 -4.3 86 115 A V H > S+ 0 0 1 26,-0.2 4,-2.2 2,-0.2 -1,-0.2 0.916 109.7 44.2 -67.2 -42.6 5.0 21.9 -0.9 87 116 A E H > S+ 0 0 42 2,-0.2 4,-1.9 1,-0.2 -2,-0.2 0.897 114.9 48.4 -66.7 -42.8 8.3 20.7 -2.5 88 117 A I H X S+ 0 0 0 -4,-2.5 4,-1.4 2,-0.2 -2,-0.2 0.925 110.3 52.5 -63.7 -43.6 7.9 23.2 -5.4 89 118 A A H ><>S+ 0 0 0 -4,-2.7 5,-2.5 1,-0.2 3,-0.8 0.949 107.3 51.7 -55.7 -49.9 7.1 26.0 -2.9 90 119 A A H ><5S+ 0 0 32 -4,-2.2 3,-1.9 1,-0.3 -1,-0.2 0.902 106.6 54.3 -53.6 -44.1 10.3 25.2 -0.9 91 120 A L H 3<5S+ 0 0 111 -4,-1.9 -1,-0.3 1,-0.3 -2,-0.2 0.814 108.5 49.7 -59.1 -32.9 12.3 25.4 -4.1 92 121 A N T <<5S- 0 0 47 -4,-1.4 -1,-0.3 -3,-0.8 -2,-0.2 0.141 123.2-104.8 -94.6 17.0 10.9 28.9 -4.6 93 122 A N T < 5S+ 0 0 130 -3,-1.9 2,-0.4 1,-0.2 126,-0.4 0.806 72.7 147.1 66.7 31.1 11.7 30.0 -1.1 94 123 A I < - 0 0 12 -5,-2.5 -1,-0.2 -6,-0.2 126,-0.2 -0.854 46.7-128.3-102.2 131.5 8.1 29.7 0.1 95 124 A K E -e 220 0A 50 124,-2.5 126,-2.5 -2,-0.4 2,-0.3 -0.523 29.0-152.6 -78.0 147.9 7.3 28.7 3.6 96 125 A T E +e 221 0A 0 37,-0.3 39,-1.7 124,-0.2 40,-1.2 -0.930 17.9 176.3-131.4 147.5 4.8 25.8 4.0 97 126 A G E -ef 222 136A 0 124,-1.9 126,-2.1 -2,-0.3 2,-0.4 -0.998 11.3-161.1-148.1 150.2 2.3 24.5 6.5 98 127 A V E -ef 223 137A 0 38,-1.9 40,-1.7 -2,-0.3 2,-0.4 -0.998 10.6-178.4-133.6 127.7 -0.3 21.7 6.9 99 128 A V E +ef 224 138A 0 124,-2.8 126,-2.4 -2,-0.4 2,-0.3 -0.986 8.3 164.4-126.7 138.3 -3.2 21.6 9.3 100 129 A A E -e 225 0A 0 38,-2.3 40,-0.2 -2,-0.4 126,-0.1 -0.996 39.8-141.5-150.8 151.9 -5.8 18.9 9.8 101 130 A T E S+ 0 0 0 124,-0.9 90,-1.6 -2,-0.3 2,-0.1 0.511 92.4 63.0 -90.9 -6.3 -8.5 17.7 12.3 102 131 A S E S- 0 0 0 1,-0.2 38,-2.3 88,-0.2 -2,-0.2 -0.388 115.9 -57.2 -93.5-174.4 -7.6 14.1 11.7 103 132 A S E > - f 0 140A 6 36,-0.2 3,-1.7 1,-0.1 38,-0.3 -0.387 51.1-122.4 -56.9 145.6 -4.2 12.5 12.4 104 133 A I T 3 S+ 0 0 0 36,-2.4 -1,-0.1 1,-0.3 37,-0.1 0.599 112.1 61.1 -70.5 -9.3 -1.6 14.5 10.5 105 134 A T T 3 S+ 0 0 0 4,-0.1 -1,-0.3 35,-0.1 20,-0.1 0.396 79.5 116.0 -94.2 0.3 -0.7 11.2 8.8 106 135 A E S <> S- 0 0 30 -3,-1.7 4,-2.5 1,-0.1 5,-0.3 -0.235 84.6 -92.5 -71.0 164.1 -4.1 10.7 7.2 107 136 A A H > S+ 0 0 1 1,-0.2 4,-2.0 2,-0.2 -1,-0.1 0.808 116.8 51.1 -48.3 -45.9 -4.5 10.7 3.4 108 137 A T H 4 S+ 0 0 0 2,-0.2 4,-0.4 1,-0.2 3,-0.3 0.980 118.0 32.7 -66.0 -59.9 -5.2 14.4 2.8 109 138 A P H >4 S+ 0 0 0 0, 0.0 3,-1.9 0, 0.0 4,-0.4 0.902 116.0 60.8 -62.8 -36.2 -2.4 16.1 4.8 110 139 A A H >X S+ 0 0 0 -4,-2.5 4,-2.8 1,-0.3 3,-1.7 0.864 94.2 62.0 -58.5 -38.3 -0.1 13.2 3.9 111 140 A S T 3< S+ 0 0 1 -4,-2.0 -1,-0.3 -3,-0.3 -3,-0.1 0.659 101.2 55.9 -63.0 -14.0 -0.4 13.9 0.2 112 141 A F T <4 S+ 0 0 0 -3,-1.9 -1,-0.3 -4,-0.4 -26,-0.2 0.532 124.9 14.2 -96.3 -7.0 1.1 17.3 0.8 113 142 A Y T <4 S+ 0 0 5 -3,-1.7 2,-0.3 -4,-0.4 -2,-0.2 0.510 115.3 59.7-139.8 -13.8 4.4 16.2 2.4 114 143 A A < - 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