==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=6-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 15-MAY-09 2WIS . COMPND 2 MOLECULE: KILLERRED; . SOURCE 2 ORGANISM_SCIENTIFIC: ANTHOMEDUSAE SP. DC-2005; . AUTHOR P.CARPENTIER,S.VIOLOT,L.BLANCHOIN,D.BOURGEOIS . 454 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19105.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 342 75.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 26 5.7 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 190 41.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 6 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 65 14.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 37 8.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 12 2.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 2 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 1 2 2 3 1 4 1 3 1 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A E 0 0 194 0, 0.0 2,-0.2 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 123.1 -27.5 -48.6 19.4 2 3 A G > + 0 0 32 82,-0.1 3,-1.3 83,-0.0 4,-0.2 -0.658 360.0 27.0-133.1-172.6 -24.9 -45.9 19.9 3 4 A G G > S- 0 0 1 80,-2.8 3,-1.7 78,-0.3 4,-0.3 -0.157 119.2 -4.6 60.7-140.8 -23.9 -42.3 19.0 4 5 A P G > S+ 0 0 38 0, 0.0 3,-0.9 0, 0.0 4,-0.3 0.605 119.5 77.9 -64.4 -14.6 -26.6 -39.7 18.1 5 6 A A G X S+ 0 0 40 -3,-1.3 3,-0.6 1,-0.3 -2,-0.2 0.806 92.0 53.9 -64.1 -29.1 -29.3 -42.4 18.4 6 7 A L G < S+ 0 0 32 -3,-1.7 3,-0.3 1,-0.2 -1,-0.3 0.652 106.9 52.3 -73.4 -17.9 -29.0 -42.0 22.1 7 8 A F G < S+ 0 0 1 -3,-0.9 -1,-0.2 -4,-0.3 -2,-0.2 0.358 74.7 98.8-105.8 5.5 -29.5 -38.2 21.8 8 9 A Q S < S+ 0 0 122 -3,-0.6 2,-0.2 -4,-0.3 -1,-0.2 0.321 82.1 65.3 -72.0 8.3 -32.8 -38.4 19.7 9 10 A S S S- 0 0 44 -3,-0.3 2,-0.2 28,-0.0 -3,-0.0 -0.733 98.6 -89.7-119.8 170.2 -34.5 -37.8 23.1 10 11 A D - 0 0 102 -2,-0.2 2,-0.3 25,-0.2 25,-0.2 -0.569 54.7-168.0 -74.9 150.3 -34.7 -35.0 25.6 11 12 A M E -A 34 0A 4 23,-2.9 23,-3.5 -2,-0.2 2,-0.2 -0.974 24.4-151.8-148.9 151.3 -32.0 -35.3 28.2 12 13 A T E -Ab 33 113A 67 100,-1.6 102,-1.6 -2,-0.3 2,-0.3 -0.664 18.3-159.5-111.4 174.8 -30.7 -34.2 31.5 13 14 A F E -Ab 32 114A 3 19,-3.0 19,-2.3 100,-0.3 2,-0.4 -0.990 7.8-148.9-157.2 158.0 -27.1 -34.1 32.7 14 15 A K E -Ab 31 115A 80 100,-3.0 102,-3.1 -2,-0.3 2,-0.4 -0.984 8.7-164.2-135.5 135.8 -25.0 -34.0 35.9 15 16 A I E -Ab 30 116A 0 15,-2.5 15,-1.1 -2,-0.4 2,-0.5 -0.976 5.3-169.5-126.0 132.4 -21.7 -32.4 36.5 16 17 A F E -Ab 29 117A 68 100,-2.9 102,-3.0 -2,-0.4 2,-0.7 -0.947 10.7-168.3-120.9 108.9 -19.3 -33.0 39.5 17 18 A I E -Ab 28 118A 0 11,-2.9 11,-1.6 -2,-0.5 2,-0.7 -0.863 10.2-172.6-105.6 113.6 -16.4 -30.5 39.7 18 19 A D E +Ab 27 119A 78 100,-3.4 102,-1.5 -2,-0.7 2,-0.2 -0.907 33.1 158.3 -94.7 111.5 -13.5 -31.2 42.0 19 20 A G E -Ab 26 120A 2 7,-3.1 7,-2.3 -2,-0.7 2,-0.3 -0.750 31.7-167.9-130.5 176.1 -11.4 -28.1 41.9 20 21 A E E -Ab 25 121A 33 100,-1.5 102,-2.6 5,-0.3 2,-0.4 -0.943 7.4-159.8-159.4 148.7 -8.8 -25.8 43.4 21 22 A V E > S-Ab 24 122A 0 3,-1.2 3,-1.2 -2,-0.3 102,-0.2 -0.878 81.8 -30.5-136.5 102.5 -7.5 -22.2 42.8 22 23 A N T 3 S- 0 0 69 100,-2.0 101,-0.1 -2,-0.4 2,-0.1 0.874 131.6 -39.6 53.5 41.8 -4.1 -21.4 44.3 23 24 A G T 3 S+ 0 0 56 99,-0.2 2,-0.5 1,-0.2 -1,-0.3 -0.337 108.5 120.4 110.4 -48.8 -4.8 -23.8 47.1 24 25 A Q E < -A 21 0A 88 -3,-1.2 -3,-1.2 -4,-0.1 -1,-0.2 -0.336 54.5-145.5 -63.8 104.9 -8.4 -23.1 47.8 25 26 A K E +A 20 0A 138 -2,-0.5 2,-0.3 -5,-0.2 -5,-0.3 -0.365 23.3 174.2 -70.2 144.2 -10.4 -26.4 47.3 26 27 A F E -A 19 0A 3 -7,-2.3 -7,-3.1 -2,-0.1 2,-0.4 -0.991 20.9-154.1-151.9 145.9 -13.9 -26.3 45.9 27 28 A T E -A 18 0A 53 -2,-0.3 21,-1.8 -9,-0.2 22,-0.9 -0.946 10.2-167.3-119.0 142.7 -16.7 -28.6 44.7 28 29 A I E -AC 17 47A 0 -11,-1.6 -11,-2.9 -2,-0.4 2,-0.5 -0.993 7.4-168.5-126.0 133.6 -19.4 -27.7 42.2 29 30 A V E +AC 16 46A 22 17,-3.9 17,-0.6 -2,-0.4 2,-0.2 -0.836 18.1 177.1-121.9 93.1 -22.5 -29.8 41.5 30 31 A A E -AC 15 45A 0 -15,-1.1 -15,-2.5 -2,-0.5 2,-0.3 -0.621 3.3-177.2 -90.6 152.0 -24.1 -28.5 38.4 31 32 A D E +AC 14 44A 58 13,-2.8 13,-3.4 -2,-0.2 2,-0.3 -0.992 11.3 142.6-146.5 149.9 -27.2 -30.0 36.9 32 33 A G E -AC 13 43A 10 -19,-2.3 -19,-3.0 -2,-0.3 2,-0.3 -0.984 31.7-123.5-167.4 178.6 -29.3 -29.3 33.8 33 34 A S E -AC 12 42A 61 9,-0.7 9,-0.8 -21,-0.3 2,-0.3 -0.890 10.7-159.8-130.3 161.7 -31.3 -30.5 30.9 34 35 A S E -AC 11 41A 2 -23,-3.5 -23,-2.9 -2,-0.3 2,-0.5 -0.984 15.0-140.2-141.3 148.2 -31.2 -30.1 27.1 35 36 A K > - 0 0 108 5,-0.6 3,-3.0 -2,-0.3 5,-0.2 -0.926 37.7 -69.1-120.4 125.6 -34.1 -30.6 24.7 36 37 A F T 3 S+ 0 0 96 -2,-0.5 -26,-0.1 1,-0.4 -28,-0.1 -0.173 124.6 29.4 -43.8 133.5 -34.0 -32.3 21.3 37 38 A P T 3 S- 0 0 53 0, 0.0 -1,-0.4 0, 0.0 34,-0.2 -0.882 99.8-164.6 -86.2 24.9 -32.6 -31.1 18.8 38 39 A H < + 0 0 12 -3,-3.0 31,-0.1 2,-0.1 30,-0.1 -0.008 46.2 121.4 54.9-167.3 -30.4 -29.6 21.6 39 40 A G S S+ 0 0 5 29,-1.5 178,-2.0 176,-0.1 2,-0.3 0.274 76.5 59.9 97.9 -12.3 -28.0 -26.6 20.8 40 41 A D E + D 0 216A 47 -5,-0.2 -5,-0.6 176,-0.2 2,-0.3 -0.915 69.2 145.0-146.3 120.0 -29.6 -24.4 23.4 41 42 A F E -CD 34 215A 7 174,-1.5 174,-2.1 -2,-0.3 2,-0.3 -0.990 35.2-130.9-157.4 159.3 -29.7 -25.4 27.0 42 43 A N E -CD 33 214A 70 -9,-0.8 -9,-0.7 -2,-0.3 2,-0.4 -0.798 12.8-159.3-113.4 152.4 -29.4 -24.2 30.6 43 44 A V E -CD 32 213A 5 170,-2.1 170,-1.9 -2,-0.3 2,-0.7 -0.998 10.7-148.7-133.1 138.2 -27.4 -25.3 33.5 44 45 A H E -CD 31 212A 82 -13,-3.4 -13,-2.8 -2,-0.4 2,-0.5 -0.936 21.6-163.2-105.4 109.5 -28.0 -24.8 37.2 45 46 A A E -CD 30 211A 0 166,-2.9 166,-2.2 -2,-0.7 2,-0.5 -0.869 8.8-177.4-104.4 121.4 -24.5 -24.6 38.8 46 47 A V E -CD 29 210A 14 -17,-0.6 -17,-3.9 -2,-0.5 2,-0.5 -0.972 20.0-142.1-124.9 120.3 -24.3 -25.0 42.6 47 48 A C E > -C 28 0A 0 162,-1.8 3,-0.6 -2,-0.5 -19,-0.3 -0.689 6.6-163.0 -77.4 126.3 -21.1 -24.8 44.7 48 49 A E T 3 S+ 0 0 88 -21,-1.8 -20,-0.2 -2,-0.5 -1,-0.1 0.456 87.6 58.3 -89.6 -2.8 -21.2 -27.4 47.5 49 50 A T T 3 S- 0 0 69 -22,-0.9 2,-1.2 1,-0.1 -1,-0.2 0.084 108.4-121.0-109.8 20.2 -18.4 -25.6 49.4 50 51 A G S < S+ 0 0 19 -3,-0.6 159,-2.7 -23,-0.1 2,-0.7 -0.656 85.2 44.9 85.2 -94.1 -20.4 -22.3 49.7 51 52 A K S S- 0 0 132 -2,-1.2 -3,-0.0 157,-0.2 157,-0.0 -0.801 72.7-137.1 -96.8 115.4 -18.3 -19.5 48.1 52 53 A L - 0 0 8 -2,-0.7 4,-0.1 1,-0.1 -26,-0.1 -0.613 21.4-144.4 -57.7 122.4 -16.7 -20.1 44.7 53 54 A P S S+ 0 0 18 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.307 75.3 32.0 -83.6 2.4 -13.3 -18.4 45.5 54 55 A M S S- 0 0 7 1,-0.1 78,-0.2 79,-0.1 77,-0.1 -0.862 107.3 -55.9-143.2 176.4 -12.8 -16.9 42.0 55 56 A S - 0 0 0 76,-1.8 -1,-0.1 -2,-0.3 4,-0.1 -0.179 34.0-139.2 -54.9 149.9 -15.0 -15.6 39.2 56 57 A W S > S+ 0 0 23 1,-0.1 3,-0.8 2,-0.1 4,-0.3 0.589 91.8 82.3 -82.7 -14.7 -17.8 -17.8 37.9 57 58 A K G > S+ 0 0 39 1,-0.2 3,-2.3 2,-0.2 4,-0.2 0.961 86.7 46.4 -58.1 -60.1 -16.9 -16.5 34.4 58 59 A P G 3 S+ 0 0 0 0, 0.0 3,-0.3 0, 0.0 -1,-0.2 0.559 104.2 67.5 -64.8 -2.6 -13.9 -18.8 33.6 59 60 A I G X> S+ 0 0 1 -3,-0.8 4,-2.0 1,-0.2 3,-0.7 0.540 71.6 95.1 -91.4 -9.0 -16.0 -21.7 34.8 60 61 A C T <4 S+ 0 0 10 -3,-2.3 -1,-0.2 -4,-0.3 3,-0.1 0.875 85.6 46.7 -47.8 -47.4 -18.4 -21.3 31.8 61 62 A H T 34 S+ 0 0 27 -3,-0.3 -1,-0.3 -4,-0.2 -2,-0.1 0.760 110.0 54.7 -73.7 -23.8 -16.6 -23.9 29.7 62 63 A L T <4 0 0 2 -3,-0.7 -1,-0.2 -4,-0.2 -2,-0.2 0.825 360.0 360.0 -74.6 -33.0 -16.5 -26.3 32.7 63 64 A I < 0 0 8 -4,-2.0 -2,-0.2 -3,-0.1 -3,-0.2 0.723 360.0 360.0 -93.3 360.0 -20.2 -26.2 33.3 64 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 65 68 A E > 0 0 14 0, 0.0 3,-1.9 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 61.0 -21.4 -30.5 27.5 66 69 A P G > + 0 0 24 0, 0.0 3,-1.2 0, 0.0 14,-0.2 0.759 360.0 64.1 -63.7 -27.7 -20.2 -30.8 23.9 67 70 A F G 3 S+ 0 0 0 1,-0.3 4,-0.1 12,-0.2 13,-0.1 0.594 92.0 67.8 -69.1 -14.2 -22.7 -33.5 22.9 68 71 A F G < S+ 0 0 0 -3,-1.9 -29,-1.5 -30,-0.1 2,-0.4 0.081 76.2 109.4 -93.4 24.0 -25.4 -30.8 23.6 69 72 A A S < S- 0 0 7 -3,-1.2 2,-0.8 -31,-0.1 149,-0.2 -0.783 77.8-115.3 -94.5 144.5 -24.3 -28.8 20.6 70 73 A R B -e 218 0A 127 147,-2.0 149,-2.4 -2,-0.4 -32,-0.1 -0.716 36.8-167.2 -79.9 112.2 -26.6 -28.6 17.5 71 74 A Y - 0 0 30 -2,-0.8 6,-0.1 147,-0.2 149,-0.1 -0.872 15.3-128.0-101.3 133.0 -24.7 -30.3 14.8 72 75 A P > - 0 0 57 0, 0.0 3,-1.4 0, 0.0 148,-0.0 -0.287 30.6-100.9 -73.5 170.7 -25.8 -30.0 11.2 73 76 A D T 3 S+ 0 0 174 1,-0.3 4,-0.1 3,-0.0 -2,-0.0 0.794 115.4 54.7 -66.8 -35.0 -26.3 -33.1 9.0 74 77 A G T 3 S+ 0 0 82 1,-0.1 2,-0.6 2,-0.1 -1,-0.3 0.470 97.8 77.7 -78.7 -0.1 -23.0 -33.0 7.0 75 78 A I S < S- 0 0 16 -3,-1.4 2,-0.1 144,-0.1 -1,-0.1 -0.946 81.1-128.7-120.5 118.4 -20.9 -32.8 10.1 76 79 A S - 0 0 18 -2,-0.6 2,-1.4 1,-0.1 3,-0.2 -0.370 19.0-136.3 -60.2 126.6 -20.2 -35.9 12.2 77 80 A H >> - 0 0 11 1,-0.2 4,-2.3 -2,-0.1 3,-0.8 -0.658 24.4-178.5 -92.0 82.6 -21.0 -35.2 15.8 78 81 A F H 3> S+ 0 0 0 -2,-1.4 4,-1.8 1,-0.2 -1,-0.2 0.860 76.2 59.3 -47.5 -50.2 -17.9 -36.7 17.6 79 82 A A H 34 S+ 0 0 3 1,-0.2 -1,-0.2 2,-0.2 -12,-0.2 0.868 113.9 38.3 -54.2 -41.1 -19.1 -35.9 21.1 80 83 A Q H X4 S+ 0 0 17 -3,-0.8 3,-1.8 1,-0.2 -77,-0.3 0.853 109.0 60.4 -75.4 -38.4 -22.2 -38.0 20.6 81 84 A E H 3< S+ 0 0 45 -4,-2.3 -78,-0.3 1,-0.3 -2,-0.2 0.781 92.4 71.1 -60.8 -24.5 -20.6 -40.7 18.5 82 85 A C T 3X S+ 0 0 0 -4,-1.8 4,-2.2 -5,-0.2 -1,-0.3 0.648 85.4 132.9 -65.5 -18.6 -18.4 -41.4 21.6 83 86 A F T <4 + 0 0 0 -3,-1.8 -80,-2.8 2,-0.3 -77,-0.1 -0.601 54.6 19.5 -92.3 150.2 -21.4 -42.9 23.4 84 87 A P T 4 S+ 0 0 70 0, 0.0 25,-0.6 0, 0.0 -1,-0.2 -0.941 130.7 47.1 -68.3 -30.6 -22.1 -45.3 25.1 85 88 A E T 4 S- 0 0 80 1,-0.1 -2,-0.3 23,-0.1 22,-0.1 0.770 106.0-147.6 -49.2 -33.3 -18.3 -45.8 25.7 86 89 A G < - 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