==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=6-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 10-MAY-07 2Z1O . COMPND 2 MOLECULE: FLUORESCENT PROTEIN DRONPA; . SOURCE 2 ORGANISM_SCIENTIFIC: ECHINOPHYLLIA SP. SC22; . AUTHOR A.KIKUCHI,J.JEYAKANTHAN,J.TAKA,Y.SHIRO,H.MIZUNO,A.MIYAWAKI . 855 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33287.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 626 73.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 6.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 408 47.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 65 7.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 59 6.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 33 3.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 7 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 7 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 4 0 8 0 4 4 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A S 0 0 122 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 141.0 61.5 40.6 52.7 2 3 A V + 0 0 51 75,-0.1 2,-0.8 78,-0.1 75,-0.0 0.555 360.0 93.3 -78.9 -7.9 58.7 43.0 51.9 3 4 A I - 0 0 7 77,-0.1 106,-0.1 74,-0.1 4,-0.0 -0.793 63.9-169.9 -89.5 112.3 59.1 44.3 55.5 4 5 A K - 0 0 104 -2,-0.8 29,-0.2 1,-0.1 3,-0.1 -0.659 35.2-110.6-102.1 159.3 61.5 47.2 55.5 5 6 A P S S+ 0 0 83 0, 0.0 2,-0.4 0, 0.0 29,-0.2 0.806 108.6 35.4 -54.8 -34.5 63.2 49.1 58.4 6 7 A D S S+ 0 0 96 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.978 75.5 163.2-128.6 120.7 61.1 52.2 57.7 7 8 A M E -A 30 0A 1 23,-2.5 23,-2.6 -2,-0.4 103,-0.2 -0.872 25.5-125.3-133.0 167.3 57.5 52.0 56.5 8 9 A K E -Ab 29 110A 100 101,-3.1 103,-2.9 -2,-0.3 2,-0.3 -0.609 12.5-147.6-110.0 169.4 54.4 54.2 56.2 9 10 A I E +Ab 28 111A 1 19,-2.3 19,-2.6 101,-0.2 2,-0.3 -0.997 13.2 176.6-139.7 141.2 50.8 54.0 57.4 10 11 A K E +Ab 27 112A 104 101,-1.9 103,-2.5 -2,-0.3 2,-0.3 -0.985 17.9 167.4-141.8 132.5 47.5 55.3 56.0 11 12 A L E -Ab 26 113A 11 15,-2.3 15,-2.3 -2,-0.3 2,-0.4 -0.981 25.8-161.3-148.6 158.1 44.1 54.6 57.6 12 13 A R E -Ab 25 114A 72 101,-1.4 103,-2.6 -2,-0.3 2,-0.5 -0.981 12.5-164.9-140.3 125.9 40.4 55.4 57.8 13 14 A M E -Ab 24 115A 0 11,-3.5 11,-2.4 -2,-0.4 2,-0.4 -0.941 4.5-175.0-112.6 133.1 38.2 54.6 60.7 14 15 A E E +Ab 23 116A 88 101,-2.2 103,-1.7 -2,-0.5 2,-0.3 -0.986 30.2 118.4-123.0 135.1 34.5 54.7 60.4 15 16 A G E -Ab 22 117A 2 7,-2.5 7,-2.1 -2,-0.4 2,-0.3 -0.942 43.9-121.5-170.1-166.4 32.5 54.1 63.6 16 17 A A E -Ab 21 118A 9 101,-2.2 103,-2.7 -2,-0.3 2,-0.4 -0.984 4.1-155.6-159.7 149.5 30.0 55.1 66.3 17 18 A V E > S-Ab 20 119A 3 3,-1.8 3,-1.7 -2,-0.3 103,-0.2 -0.991 87.3 -12.8-131.2 121.7 29.8 55.5 70.0 18 19 A N T 3 S- 0 0 9 101,-2.7 102,-0.2 -2,-0.4 -1,-0.1 0.875 132.2 -52.7 53.7 39.4 26.4 55.2 71.7 19 20 A G T 3 S+ 0 0 34 100,-0.6 -1,-0.3 1,-0.2 101,-0.1 0.424 110.0 121.5 81.8 -0.9 24.8 55.5 68.3 20 21 A H E < -A 17 0A 125 -3,-1.7 -3,-1.8 26,-0.0 -1,-0.2 -0.867 54.7-139.3-103.0 115.2 26.6 58.6 67.2 21 22 A P E +A 16 0A 90 0, 0.0 2,-0.3 0, 0.0 -5,-0.3 -0.290 28.3 159.6 -72.5 151.1 28.7 58.2 64.0 22 23 A F E -A 15 0A 6 -7,-2.1 -7,-2.5 -2,-0.1 2,-0.3 -0.964 22.1-148.0-160.1 167.8 32.1 59.6 63.2 23 24 A A E -AC 14 45A 0 22,-2.1 21,-2.9 -2,-0.3 22,-1.8 -0.992 10.4-173.2-148.2 140.0 35.1 59.1 60.9 24 25 A I E -AC 13 43A 0 -11,-2.4 -11,-3.5 -2,-0.3 2,-0.3 -0.988 4.8-162.3-136.1 142.1 38.8 59.6 61.3 25 26 A E E -AC 12 42A 76 17,-2.4 17,-3.0 -2,-0.4 2,-0.3 -0.920 11.6-172.7-123.1 151.7 41.7 59.4 58.9 26 27 A G E -AC 11 41A 5 -15,-2.3 -15,-2.3 -2,-0.3 2,-0.4 -0.990 17.2-163.7-147.2 155.4 45.4 59.1 59.7 27 28 A V E +AC 10 40A 94 13,-2.0 13,-2.9 -2,-0.3 2,-0.3 -0.997 30.3 140.9-134.8 136.4 48.8 59.1 58.2 28 29 A G E -AC 9 39A 9 -19,-2.6 -19,-2.3 -2,-0.4 2,-0.3 -0.858 32.0-136.0-156.6-168.2 51.9 57.8 59.9 29 30 A L E +AC 8 38A 84 9,-2.3 9,-2.4 -21,-0.3 2,-0.3 -0.974 20.2 170.2-156.3 162.2 55.2 55.9 59.8 30 31 A G E -AC 7 37A 2 -23,-2.6 -23,-2.5 -2,-0.3 7,-0.3 -0.982 36.8-111.6-166.1 176.4 57.1 53.4 61.9 31 32 A K E >>> - C 0 36A 69 5,-3.1 5,-1.6 -2,-0.3 4,-1.5 -0.897 24.1-166.7-123.5 95.7 60.0 50.9 62.3 32 33 A P T 345S+ 0 0 0 0, 0.0 34,-0.6 0, 0.0 -1,-0.1 0.846 88.3 47.2 -50.0 -44.1 58.6 47.3 62.3 33 34 A F T 345S+ 0 0 103 1,-0.2 -27,-0.1 -29,-0.2 32,-0.1 0.694 115.2 45.7 -76.7 -17.9 61.8 45.8 63.6 34 35 A E T <45S- 0 0 89 -3,-1.0 33,-0.3 -29,-0.2 -1,-0.2 0.595 106.1-128.5 -96.2 -14.1 62.1 48.3 66.3 35 36 A G T <5 + 0 0 0 -4,-1.5 175,-2.4 31,-0.3 2,-0.4 0.843 64.4 127.6 70.4 35.5 58.5 48.1 67.3 36 37 A K E < +CD 31 209A 111 -5,-1.6 -5,-3.1 173,-0.2 2,-0.3 -0.961 26.3 159.4-128.4 143.4 57.9 51.8 67.1 37 38 A Q E -CD 30 208A 13 171,-2.1 171,-2.3 -2,-0.4 2,-0.3 -0.993 13.3-172.9-156.5 156.8 55.3 53.9 65.3 38 39 A S E +CD 29 207A 28 -9,-2.4 -9,-2.3 -2,-0.3 2,-0.3 -0.975 9.9 179.1-148.7 156.7 53.7 57.3 65.3 39 40 A M E -CD 28 206A 5 167,-2.0 167,-2.8 -2,-0.3 2,-0.5 -0.994 27.0-126.8-160.1 156.5 50.8 58.9 63.4 40 41 A D E -CD 27 205A 97 -13,-2.9 -13,-2.0 -2,-0.3 2,-0.4 -0.939 28.7-162.4-109.2 126.1 48.7 62.0 62.9 41 42 A L E -CD 26 204A 1 163,-3.2 163,-2.2 -2,-0.5 2,-0.4 -0.893 5.5-167.9-113.3 140.3 45.0 61.6 63.1 42 43 A K E -CD 25 203A 117 -17,-3.0 -17,-2.4 -2,-0.4 2,-0.4 -0.991 22.2-129.3-131.2 128.8 42.3 63.9 61.9 43 44 A V E +C 24 0A 25 159,-2.2 -19,-0.3 -2,-0.4 3,-0.1 -0.623 28.9 173.4 -74.7 124.2 38.5 63.8 62.6 44 45 A K E + 0 0 115 -21,-2.9 2,-0.3 -2,-0.4 -20,-0.2 0.694 63.9 17.3-103.4 -27.8 36.7 64.0 59.3 45 46 A E E S+C 23 0A 106 -22,-1.8 -22,-2.1 1,-0.1 -1,-0.3 -0.991 114.0 28.3-145.9 149.0 33.1 63.4 60.4 46 47 A G S S- 0 0 42 -2,-0.3 -1,-0.1 -24,-0.3 -22,-0.0 0.530 86.5-171.5 81.2 7.7 31.2 63.6 63.7 47 48 A G + 0 0 48 2,-0.1 -1,-0.2 -24,-0.1 2,-0.1 -0.495 58.2 50.4 -87.1 157.4 33.4 66.2 65.4 48 49 A P S S- 0 0 84 0, 0.0 153,-0.1 0, 0.0 -1,-0.1 0.450 92.0-131.1 -73.6 135.5 33.8 67.5 68.0 49 50 A L - 0 0 4 151,-0.5 -2,-0.1 1,-0.1 4,-0.1 -0.321 10.6-152.2 -57.3 130.5 33.9 63.9 69.3 50 51 A P S S+ 0 0 59 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.339 70.8 61.7 -87.8 7.3 31.6 63.7 72.4 51 52 A F S S- 0 0 6 79,-0.1 2,-0.2 80,-0.1 78,-0.2 -0.851 100.0 -73.3-131.2 166.1 33.6 60.9 74.0 52 53 A A > - 0 0 3 76,-1.9 3,-1.5 -2,-0.3 4,-0.3 -0.395 35.5-144.7 -60.7 121.4 37.1 60.2 75.4 53 54 A Y T >> S+ 0 0 8 1,-0.3 3,-2.1 -2,-0.2 4,-0.8 0.783 89.3 78.7 -58.6 -28.1 39.5 59.9 72.5 54 55 A D H 3> S+ 0 0 13 1,-0.3 4,-0.5 2,-0.2 -1,-0.3 0.740 78.9 66.7 -56.8 -26.2 41.5 57.2 74.4 55 56 A I H <4 S+ 0 0 0 -3,-1.5 -1,-0.3 1,-0.2 -2,-0.2 0.750 104.3 48.2 -68.6 -16.8 39.0 54.5 73.7 56 57 A L H X> S+ 0 0 0 -3,-2.1 3,-1.8 -4,-0.3 4,-1.1 0.759 88.9 81.7 -91.1 -28.5 40.1 54.8 70.0 57 58 A T H 3< S+ 0 0 2 -4,-0.8 3,-0.3 1,-0.3 -2,-0.1 0.797 85.6 53.3 -54.1 -41.9 43.8 54.8 70.2 58 59 A T T 3< S+ 0 0 26 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.576 106.3 57.7 -72.7 -6.0 44.6 51.0 70.5 59 60 A V T <4 0 0 0 -3,-1.8 -1,-0.2 1,-0.1 -2,-0.2 0.810 360.0 360.0 -89.4 -33.9 42.6 50.5 67.4 60 61 A F < 0 0 8 -4,-1.1 -2,-0.2 -3,-0.3 -3,-0.1 0.836 360.0 360.0 -74.5 360.0 44.7 52.9 65.3 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 65 A N > 0 0 7 0, 0.0 3,-2.0 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 102.6 49.0 46.7 63.9 63 66 A R T 3 + 0 0 11 1,-0.3 3,-0.2 2,-0.0 13,-0.2 0.432 360.0 86.1 -69.5 2.7 51.2 44.2 65.6 64 67 A V T 3 S+ 0 0 0 1,-0.3 2,-1.5 12,-0.2 -1,-0.3 0.806 80.9 69.5 -70.0 -23.8 53.6 44.2 62.7 65 68 A F S < S+ 0 0 1 -3,-2.0 2,-0.3 -30,-0.1 -1,-0.3 -0.542 79.4 101.6 -91.3 68.7 55.0 47.1 64.7 66 69 A A S S- 0 0 0 -2,-1.5 2,-0.8 -34,-0.6 -31,-0.3 -0.969 77.3-116.5-151.0 134.3 56.3 45.0 67.5 67 70 A K B -e 211 0A 101 143,-2.4 145,-3.0 -33,-0.3 -34,-0.1 -0.665 34.4-166.8 -78.9 110.1 59.9 44.0 68.0 68 71 A Y - 0 0 28 -2,-0.8 6,-0.1 143,-0.2 145,-0.1 -0.873 14.6-133.8-102.2 124.9 60.0 40.2 67.8 69 72 A P > - 0 0 50 0, 0.0 3,-1.6 0, 0.0 144,-0.0 -0.319 28.5-109.0 -69.3 159.3 63.1 38.3 69.0 70 73 A E T 3 S+ 0 0 205 1,-0.3 4,-0.1 3,-0.0 -2,-0.0 0.749 114.8 62.2 -63.1 -24.0 64.4 35.6 66.6 71 74 A N T 3 S+ 0 0 124 2,-0.1 2,-0.4 143,-0.0 -1,-0.3 0.331 93.8 72.6 -87.0 8.4 63.3 32.8 69.0 72 75 A I S < S- 0 0 16 -3,-1.6 2,-0.2 111,-0.1 140,-0.1 -0.987 87.9-117.0-124.0 131.1 59.6 33.7 68.7 73 76 A V - 0 0 34 -2,-0.4 2,-1.0 1,-0.1 -2,-0.1 -0.494 24.3-142.5 -66.4 129.9 57.6 33.1 65.6 74 77 A D > + 0 0 24 -2,-0.2 4,-1.4 1,-0.2 5,-0.1 -0.788 20.8 179.9-100.3 93.4 56.4 36.4 64.1 75 78 A Y H > S+ 0 0 8 -2,-1.0 4,-0.8 1,-0.2 -1,-0.2 0.856 78.1 50.2 -58.1 -39.7 52.8 35.8 62.8 76 79 A F H >4 S+ 0 0 0 2,-0.2 3,-0.7 1,-0.2 4,-0.4 0.922 108.3 45.1 -72.4 -49.4 52.5 39.4 61.7 77 80 A K H >4 S+ 0 0 25 1,-0.2 3,-0.8 -14,-0.2 -1,-0.2 0.825 112.1 57.3 -67.2 -25.5 55.6 40.0 59.6 78 81 A Q H 3< S+ 0 0 79 -4,-1.4 -1,-0.2 1,-0.2 -2,-0.2 0.768 93.1 69.5 -71.0 -28.7 55.0 36.6 58.0 79 82 A S T - G 0 168A 1 4,-2.6 3,-2.1 -2,-0.7 76,-0.3 -0.563 26.0-117.4 -83.7 147.7 35.0 48.1 80.1 93 96 A E T 3 S+ 0 0 75 74,-2.7 75,-0.1 1,-0.3 -1,-0.1 0.673 111.4 54.1 -59.4 -19.8 34.3 47.6 83.8 94 97 A D T 3 S- 0 0 53 73,-0.2 -1,-0.3 2,-0.1 3,-0.1 0.224 125.8 -93.2 -99.8 15.8 31.2 49.8 83.8 95 98 A G S < S+ 0 0 27 -3,-2.1 2,-0.2 1,-0.3 -2,-0.1 0.325 78.7 138.8 96.8 -11.0 29.5 48.0 80.9 96 99 A G - 0 0 0 -5,-0.1 -4,-2.6 23,-0.1 2,-0.4 -0.534 34.4-165.6 -67.6 133.9 30.7 50.0 78.0 97 100 A I E -FH 91 120A 17 23,-2.4 23,-2.3 -2,-0.2 2,-0.5 -0.989 9.4-169.6-128.4 127.7 31.6 47.6 75.2 98 101 A C E -FH 90 119A 0 -8,-2.4 -8,-2.9 -2,-0.4 2,-0.4 -0.967 1.9-168.2-119.3 129.0 33.6 48.4 72.0 99 102 A N E +FH 89 118A 3 19,-2.8 19,-3.0 -2,-0.5 2,-0.3 -0.929 16.2 173.2-111.2 136.6 33.9 46.1 69.0 100 103 A A E -FH 88 117A 1 -12,-2.5 -12,-2.4 -2,-0.4 2,-0.3 -0.979 13.9-170.3-144.0 155.4 36.5 47.0 66.4 101 104 A T E -FH 87 116A 20 15,-2.1 15,-1.9 -2,-0.3 2,-0.4 -0.978 2.8-167.0-142.4 154.4 38.1 45.5 63.3 102 105 A N E -FH 86 115A 2 -16,-2.0 -16,-2.1 -2,-0.3 2,-0.6 -0.972 7.5-160.9-146.0 126.7 41.0 46.3 61.0 103 106 A D E -FH 85 114A 59 11,-1.9 11,-1.6 -2,-0.4 2,-0.6 -0.955 20.7-160.1-106.7 115.6 41.7 44.9 57.6 104 107 A I E +FH 84 113A 0 -20,-2.5 -20,-1.0 -2,-0.6 2,-0.2 -0.881 17.3 165.7-108.0 122.5 45.4 45.5 56.9 105 108 A T E - 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