==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=29-APR-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER LUMINESCENT PROTEIN 21-APR-08 2ZMW . COMPND 2 MOLECULE: FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: FUNGIA CONCINNA; . AUTHOR A.KIKUCHI,E.FUKUMURA,S.KARASAWA,H.MIZUNO,A.MIYAWAKI,Y.SHIRO, . 852 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 37576.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 611 71.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 50 5.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 408 47.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 16 1.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 54 6.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 68 8.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 24 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 3 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 4 0 8 0 4 4 4 8 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A V 0 0 195 0, 0.0 2,-0.2 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 136.0 44.9 -29.1 43.5 2 3 A S - 0 0 61 1,-0.1 3,-0.1 3,-0.0 0, 0.0 -0.709 360.0-176.2-156.1 96.3 42.8 -26.0 43.3 3 4 A V S S+ 0 0 58 1,-0.2 2,-0.5 -2,-0.2 -1,-0.1 0.909 83.5 32.1 -60.1 -45.7 42.9 -23.7 40.3 4 5 A I S S- 0 0 3 78,-0.1 -1,-0.2 4,-0.0 4,-0.1 -0.962 80.0-158.9-119.2 118.4 40.6 -21.1 41.7 5 6 A K - 0 0 90 -2,-0.5 29,-0.2 2,-0.2 -3,-0.0 -0.498 34.9-103.4 -94.4 164.1 40.5 -20.6 45.5 6 7 A P S S+ 0 0 66 0, 0.0 2,-0.4 0, 0.0 29,-0.2 0.746 114.5 41.7 -53.6 -25.6 37.8 -19.0 47.8 7 8 A E S S+ 0 0 132 25,-0.2 2,-0.3 26,-0.1 -2,-0.2 -0.995 74.6 163.5-127.9 130.0 40.0 -15.9 47.8 8 9 A M E -A 31 0A 4 23,-2.0 23,-2.2 -2,-0.4 2,-0.3 -0.918 26.1-129.9-140.4 165.6 41.8 -14.5 44.8 9 10 A K E -Ab 30 112A 104 102,-3.1 104,-2.5 -2,-0.3 2,-0.3 -0.738 15.8-152.9-114.1 164.2 43.4 -11.3 43.7 10 11 A M E -Ab 29 113A 2 19,-2.2 19,-2.2 -2,-0.3 2,-0.4 -0.999 8.5-167.0-141.4 142.5 43.0 -9.2 40.5 11 12 A R E -Ab 28 114A 143 102,-2.5 104,-2.9 -2,-0.3 2,-0.4 -0.999 17.8-159.5-128.0 128.8 45.2 -6.8 38.5 12 13 A Y E +Ab 27 115A 23 15,-2.7 15,-1.7 -2,-0.4 2,-0.3 -0.884 16.5 172.7-116.9 143.1 43.6 -4.6 35.9 13 14 A Y E -Ab 26 116A 79 102,-2.4 104,-2.5 -2,-0.4 2,-0.4 -0.947 7.6-179.5-147.5 123.6 44.9 -2.7 32.8 14 15 A M E -Ab 25 117A 9 11,-2.8 11,-1.9 -2,-0.3 2,-0.6 -0.982 18.3-160.2-129.2 138.1 42.8 -0.9 30.2 15 16 A D E +Ab 24 118A 38 102,-2.3 104,-2.4 -2,-0.4 2,-0.3 -0.969 40.0 157.8-108.6 118.8 43.7 1.0 27.1 16 17 A G E -Ab 23 119A 8 7,-2.9 7,-2.7 -2,-0.6 2,-0.3 -0.897 33.0-171.1-145.0 172.9 40.7 3.2 26.2 17 18 A S E -Ab 22 120A 0 102,-1.5 104,-3.1 -2,-0.3 2,-0.4 -0.936 3.3-171.7-161.7 140.4 39.2 6.2 24.5 18 19 A V E > S-Ab 21 121A 0 3,-1.7 3,-2.4 -2,-0.3 104,-0.2 -0.990 79.9 -12.4-138.6 124.8 35.8 7.8 24.7 19 20 A N T 3 S- 0 0 11 102,-2.0 103,-0.1 -2,-0.4 3,-0.1 0.849 131.2 -53.0 52.6 36.6 34.8 10.6 22.3 20 21 A G T 3 S+ 0 0 10 101,-0.5 2,-0.8 1,-0.2 -1,-0.3 0.438 107.3 128.4 82.2 -2.1 38.4 10.9 21.3 21 22 A H E < -A 18 0A 85 -3,-2.4 -3,-1.7 26,-0.0 2,-0.2 -0.812 49.0-150.6 -90.3 111.5 39.7 11.3 24.9 22 23 A E E +A 17 0A 37 -2,-0.8 25,-2.9 -5,-0.2 26,-0.4 -0.578 27.7 150.9 -84.4 144.4 42.5 8.7 25.3 23 24 A F E -AC 16 46A 7 -7,-2.7 -7,-2.9 23,-0.3 2,-0.3 -0.981 33.8-135.2-162.5 168.7 43.2 7.1 28.7 24 25 A T E -AC 15 45A 40 21,-1.9 21,-2.7 -2,-0.3 2,-0.4 -0.964 12.7-154.3-132.2 147.5 44.4 4.1 30.6 25 26 A I E +AC 14 44A 9 -11,-1.9 -11,-2.8 -2,-0.3 2,-0.3 -0.991 15.1 175.2-124.3 134.6 43.0 2.4 33.7 26 27 A E E +AC 13 43A 96 17,-2.0 17,-3.3 -2,-0.4 2,-0.3 -0.986 14.6 135.1-136.2 144.9 45.0 0.4 36.2 27 28 A G E -AC 12 42A 4 -15,-1.7 -15,-2.7 -2,-0.3 2,-0.3 -0.984 33.7-133.6-171.8 179.9 43.9 -1.2 39.4 28 29 A E E +AC 11 41A 83 13,-1.6 13,-2.2 -2,-0.3 2,-0.3 -0.980 23.2 164.6-148.5 154.8 43.9 -4.1 41.8 29 30 A G E -AC 10 40A 9 -19,-2.2 -19,-2.2 -2,-0.3 2,-0.3 -0.910 24.8-131.4-157.2-176.5 41.3 -5.9 43.8 30 31 A T E +AC 9 39A 80 9,-2.2 9,-2.5 -2,-0.3 2,-0.3 -0.969 28.8 167.4-143.4 152.2 40.4 -9.0 45.8 31 32 A G E -AC 8 38A 6 -23,-2.2 -23,-2.0 -2,-0.3 7,-0.3 -0.966 39.3-115.6-159.7 175.8 37.4 -11.3 45.6 32 33 A R E >>> - C 0 37A 79 5,-2.8 5,-2.0 -2,-0.3 3,-1.1 -0.878 24.7-169.1-122.6 93.1 35.5 -14.5 46.5 33 34 A P G >45S+ 0 0 0 0, 0.0 3,-1.3 0, 0.0 35,-0.4 0.893 86.6 46.0 -47.8 -53.3 34.9 -16.4 43.2 34 35 A Y G 345S+ 0 0 113 1,-0.3 36,-0.1 -29,-0.2 33,-0.1 0.661 114.8 48.6 -70.2 -15.3 32.5 -19.0 44.7 35 36 A E G <45S- 0 0 101 -3,-1.1 34,-0.3 -29,-0.2 -1,-0.3 0.391 108.5-123.4-103.1 1.9 30.6 -16.3 46.5 36 37 A G T <<5 + 0 0 0 -3,-1.3 174,-2.4 -4,-0.6 2,-0.4 0.843 66.2 134.2 60.1 37.3 30.3 -14.1 43.4 37 38 A H E < +CD 32 209A 39 -5,-2.0 -5,-2.8 172,-0.2 2,-0.3 -0.938 25.8 165.6-124.2 140.3 31.9 -11.2 45.2 38 39 A Q E -CD 31 208A 5 170,-1.8 170,-2.2 -2,-0.4 2,-0.3 -0.996 10.3-174.0-152.0 146.8 34.7 -8.7 44.1 39 40 A E E +CD 30 207A 97 -9,-2.5 -9,-2.2 -2,-0.3 2,-0.3 -0.989 17.2 158.0-138.6 145.1 36.1 -5.4 45.3 40 41 A M E -CD 29 206A 18 166,-2.0 166,-2.1 -2,-0.3 2,-0.4 -0.981 34.1-130.0-161.5 169.4 38.7 -3.2 43.7 41 42 A T E -CD 28 205A 49 -13,-2.2 -13,-1.6 -2,-0.3 2,-0.4 -0.999 26.2-166.9-129.3 128.6 40.2 0.2 43.3 42 43 A L E -CD 27 204A 13 162,-3.3 162,-2.5 -2,-0.4 2,-0.5 -0.956 8.4-154.1-124.7 139.2 40.8 1.7 39.9 43 44 A R E -CD 26 203A 93 -17,-3.3 -17,-2.0 -2,-0.4 2,-0.5 -0.939 7.3-154.1-115.5 125.4 42.9 4.6 38.8 44 45 A V E -C 25 0A 5 158,-3.1 2,-0.5 -2,-0.5 -19,-0.2 -0.846 9.0-172.5-100.1 127.4 42.1 6.6 35.6 45 46 A T E -C 24 0A 81 -21,-2.7 -21,-1.9 -2,-0.5 2,-0.2 -0.954 14.8-145.1-122.7 111.8 44.9 8.5 33.8 46 47 A M E > -C 23 0A 14 4,-0.6 3,-1.8 -2,-0.5 -23,-0.3 -0.500 18.0-127.4 -74.1 141.8 44.0 10.7 30.9 47 48 A A T 3 S+ 0 0 74 -25,-2.9 -24,-0.1 1,-0.3 -1,-0.1 0.866 113.9 38.3 -56.5 -38.0 46.5 10.9 28.1 48 49 A K T 3 S- 0 0 204 -26,-0.4 -1,-0.3 1,-0.3 -25,-0.1 0.261 117.7-120.4 -96.7 11.7 46.5 14.7 28.3 49 50 A G < + 0 0 43 -3,-1.8 -1,-0.3 1,-0.2 -2,-0.1 -0.111 67.0 92.5 74.0 179.5 46.3 14.6 32.1 50 51 A G S S- 0 0 50 -4,-0.1 -4,-0.6 -3,-0.1 -1,-0.2 -0.212 82.1 -31.3 89.4 177.5 43.4 16.2 33.9 51 52 A P S S- 0 0 105 0, 0.0 -6,-0.1 0, 0.0 150,-0.0 -0.342 71.3 -95.8 -70.9 150.1 40.1 14.8 35.2 52 53 A M - 0 0 23 1,-0.1 4,-0.1 -8,-0.1 -2,-0.1 -0.535 31.6-143.6 -67.4 127.9 38.3 12.0 33.3 53 54 A P S S+ 0 0 39 0, 0.0 2,-0.3 0, 0.0 80,-0.2 0.097 73.9 54.9 -81.9 22.2 35.7 13.7 31.0 54 55 A F S S- 0 0 0 78,-0.1 77,-0.2 79,-0.1 2,-0.1 -0.988 93.0 -88.2-152.3 157.1 33.1 11.0 31.4 55 56 A A > - 0 0 0 75,-2.0 3,-1.8 -2,-0.3 4,-0.2 -0.443 34.2-130.0 -66.7 137.4 31.1 9.1 34.1 56 57 A F T >> S+ 0 0 2 1,-0.3 3,-2.0 2,-0.2 4,-1.8 0.709 99.9 80.6 -62.4 -20.9 33.0 6.0 35.3 57 58 A D H 3> S+ 0 0 0 1,-0.3 4,-0.6 2,-0.2 -1,-0.3 0.809 78.4 71.5 -55.4 -28.6 29.8 4.0 34.8 58 59 A L H <4 S+ 0 0 0 -3,-1.8 -1,-0.3 1,-0.2 -2,-0.2 0.793 116.8 17.8 -59.2 -28.8 30.8 3.9 31.1 59 60 A V H X4 S+ 0 0 7 -3,-2.0 3,-2.1 -4,-0.2 -2,-0.2 0.484 97.4 95.2-121.8 -6.8 33.6 1.5 32.0 60 61 A S H 3< S+ 0 0 8 -4,-1.8 -3,-0.1 1,-0.3 -2,-0.1 0.834 80.2 61.5 -55.9 -36.5 32.7 0.1 35.4 61 62 A H T 3< 0 0 22 -4,-0.6 -1,-0.3 1,-0.2 -2,-0.1 0.511 360.0 360.0 -68.5 -6.2 31.1 -3.0 33.9 62 63 A V < 0 0 24 -3,-2.1 -1,-0.2 2,-0.0 -2,-0.2 0.860 360.0 360.0 -85.1 360.0 34.4 -3.9 32.3 63 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 64 68 A H > 0 0 13 0, 0.0 3,-2.2 0, 0.0 -2,-0.0 0.000 360.0 360.0 360.0 71.4 35.1 -10.8 35.6 65 69 A R T 3 + 0 0 11 1,-0.3 3,-0.2 2,-0.0 14,-0.2 0.662 360.0 78.8 -57.7 -20.6 32.6 -13.6 35.4 66 70 A P T 3 S+ 0 0 0 0, 0.0 2,-1.5 0, 0.0 -1,-0.3 0.843 85.1 70.9 -58.9 -22.8 34.7 -16.1 37.3 67 71 A F S < S+ 0 0 0 -3,-2.2 2,-0.4 -31,-0.1 143,-0.2 -0.493 75.4 114.8 -90.5 65.0 33.5 -14.1 40.2 68 72 A T - 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