*** W.e1: 11.72/131.6 sees 8.12, 7.29, 7.04, 6.95, 6.84, 6.63, 6.54, 5.72, 5.58, 4.18, 3.36, 3.04, 2.85, 2.59, 2.46, 2.35, 2.24, 2.10, 1.91, 1.76 11.53/130.8 sees 8.11, 7.28, 7.10, 6.99, 5.59, 4.16, 3.19, 3.06, 2.72, 2.44, 2.30, 2.09, 1.91, 1.81 9.91/127.7 sees 11.46, 8.60, 8.10, 7.43, 7.26, 7.13, 7.02, 6.88, 5.34,.4.84, 4.64, 4.38, 4.13, 3.87, 3.42, 3.24, 3.11, 2.90, 2.50, 2.27, 2.06, 1.84, 1.39, 1.01, -0.08(= A127.HB#?) ***important: D30.HN: A127.HN = unic (strong) H125.HN = unic (weak) Q126.HN or A124.HN or E135.HN or N97.HN (weak) D30.HN sees E137.HN or aro (weak) D30.HN sees N107.HA or aro (weak) D30.HN sees Q126.HA or ... (strong) D30.HN sees Q126.HG1 or ... (medium) R31.HN: A127.HN = unic (weak) R31.HN; H125.HN = unic (strong) R31.HN sees Q126.HN or A124.HN or E135.HN or N97.HN or ... (strong) beta2 (43-52) has a kink around S49? A36.HN: N107.HA = unic (medium) D38.HN: N107.HA = unic (weak) Y41.HN: N107.HN or ... (medium) Y41.aro: 5.93, 6.61, 7.76 (less probable)? W54.HN: V104.HG2# or A127.HB# or L122.HD2# or L27.HG or L108.HD2# C66.HN: sees 12.518 and 11.513 (His sc) same as unassigned residue 8.31/103.9 sees 8.25 ppm, i.e., N1-PVB or ... (strong) sees 8.97 ppm, i.e., N5-PVB or R68.HN or I62.HN or ... (very weak) sees 5.59 ppm, i.e., T6-PVB or the impossible=C29.HA (strong) sees 2.27 ppm, i.e., M11-PVB or P65.HB1 or ... (medium) sees 2.07 ppm, i.e., M9-PVB or M10-PVB or ... (medium) sees 1.92 ppm, i.e., M8-PVB or P65.HB2 or ... (medium) sees 1.52 ppm, i.e., M6-PVB or P65.HG2 or ... (strong) F67.HN: sees 5.58 ppm, i.e., T6-PVB or the impossible=C29.HA (medium) F67.aro=6.64? R68.HN: sees 2.27 ppm, i.e., M11-PVB or P65.HB1 or ... (weak) sees 1.61 ppm, i.e., M7-PVB or ... (weak) H70.HN: sees 11.715 (His sc) (very weak) sees 5.59 ppm, i.e., T6-PVB or the impossible=C29.HA (medium) A74.HN: sees 5.59 ppm, i.e., T6-PVB or the impossible=C29.HA (strong) G78.HN: Q117.HA or L118.HA or F119.HA or ... (medium) D116.HA or ... (medium) D115.HB1 or ... (medium) Q117.HB1 or R76.HB1 or ... (medium) I114.HD1# or R79.HB2 or ... (medium) R79.HN: D116.HB1 or D115.HB1 or ... (medium) Q81.HN: V110.HA or ... (strong) L108.HA or ... (medium) M112.HA or ... (weak) A82.HN: L108.HA or A106.HA or ... (medium) T83.HN: L108.HN or ... (weak) N107.HN or ... (strong) T84.HN: N107.HN or ... (weak) D85.HN: K103.HN or ... (medium) G90.HN: only a few, very weak peaks L91.HB: 2 configurations T92.HN: 6.14/107.5 has a conf. with diff. n15noes at 6.32/107.8 T92.HN: check tocsy for HA/HB/HG2# at 4.29/4.42/1.36 C94.HN: can't see HA, HB1, HB2; wrong assigned??? H95.HN: sees 11.49 (His sc) N97.HN: tons of strong peaks cannot be assigned!!! L102.HN: Q134.HN = unic (strong) E135.HA or ... (medium) Q134.HG2 or Q134.HB1 or ... (strong) Q132.HG2 or K103.HB2 or ... (medium) K103.HN: Q134.HG2 or Q134.HB1 or ... (medium) A106.HN: Q126.HN or A124.HN or ... (medium) S128.HN or K103.HN or ... (medium) N97.HN or ... (medium) L96.HN or ... (weak) D85.HB1 or ... (medium) D85.HB2 or ... (medium) H125.HB2 or ... (medium) N107.HN: T83.HN=unic (medium) L108.HN: A124.HN or Q126.HN or ... (medium) H125.HD2 or ... (weak) H125.HA or ... (strong) V110.HN: A82.HN or V109.HN or T84.HN or ... (medium) Q81.HA or I80.HA or ... (medium) M112.HN: Y75.HN or I114.HN or ... (medium) Y75.HE1 or ... (medium) G120.HN: V110.HG1# or L121.HB2 or V113.HG1# or ... (strong) L122.HN: V109.HN or R105.HN or ... (weak) A124.HN: L108.HD2# or V104.HG2# or ... (medium) L108.HG or ... (medium) Q126.HN: R105.HN or V109.HN (weak) K103.HN or ... (medium) R105.HD1 or N107.HB1 or K103.HE2 or V104.HA or ... (medium) HB1/HB2/HG2 = 2.65, 2.13, 1.83 V104.HB# or K103.HG2 or R105.HB1 or ... (medium) V104.HG1# or L108.HB1 or ... (medium) V104.HG2# or L108.HD1# or ... (medium) E129.HN: HA/HB1/HB2/HG1/HG2 = 4.64, 2.37, 2.20, 1.81 R131.HN: sees D85.HN, HA, HB1, HB2? Q132.HN: sees D85, I86, F87, K88? W133.HN: sees R31, D30, A89, F87, D85? Q134.HN: sees F87, D85, D30? V151.HN: sees 8.79: HN of L152, E153? sees 4.17: HA of V151, L152, E153? sees 3.14: HA of V151, L152, E153? sees 2.34, 1.75, 1.12, 0.91, 0.63, 0.46 = V151,L152,E153:HBs,HGs,HDs? L155.HN: sees 4.84, 4.31, 1.78. 1.63, 1.41, 1.21, 1.08, 0.99, 0.87, 0.44, 0.29: s.c. of L155, W154, H156, etc.? ***Q?: A124.HN; N107.HA, L108.HB2 A28.HN: Q134.HG1, Q132.HB2, I136.HG2# or I138,HG2#, I138.HD1#, A127.HB# F16.aro: 7.01 (6.9-7.1)? E17.HN: F119.HB2? E17: HB1/HB2/HG1/HG2=2.78, 2.56, 2.17, 2.03? A21.HN: S128.HN or R131.HN, Q140.HN or Q132.HN or S149.HN or E143.HN or R100.HN, K88.HN or A145.HN or Q77.HN R24.HN: W133.HD1, A124.HA or E48.HA or V32.HA, I123.HA or V110.HA or V50.HA R24: HD2=3.25? ***notes: E8: disappeared L9: very weak L9: HA=4.22, 4.08?; HB1,2/HG=1.90, 1.79? I15: acoperit de K88 L19: weak, acoperit ***unassigned: 8.34/105.0 8.31/103.9 sees: 11.497 and 15.525 (His sc) ***check: A3.HN = 8.60, 9.34, 9.06(from A2 and V4)