BMRB Entry 15647
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15647
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Title: 1H chemical shift and NMR structure of Herpes simplex virus type 1 (HSV-1)fusion peptide gH626-644 PubMed: 18678872
Deposition date: 2008-02-03 Original release date: 2008-08-22
Authors: Raiola, Luca; Falanga, Annarita; Isernia, Carla; Fattorusso, Roberto; Galdiero, Stefania; Pedone, Carlo
Citation: Galdiero, Stefania; Falanga, Annarita; Vitiello, Mariateresa; Raiola, Luca; Fattorusso, Roberto; Browne, Helena; Pedone, Carlo; Isernia, Carla; Galdiero, Massimiliano. "Analysis of a membrane interacting region of herpes simplex virus type 1 glycoprotein H" J. Biol. Chem. 283, 29993-30009 (2008).
Assembly members:
HSV-1_gH_protein,_gH626-644_fusion_peptide, polymer, 19 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 10298 Superkingdom: Viruses Kingdom: not available Genus/species: Simplexvirus Human herpesvirus 1
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HSV-1_gH_protein,_gH626-644_fusion_peptide: GLASTLTRWAHYNALIRAF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 140 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HSV-1 gH protein, gH626-644 fusion peptide | 1 |
Entities:
Entity 1, HSV-1 gH protein, gH626-644 fusion peptide 19 residues - Formula weight is not available
| 1 | GLY | LEU | ALA | SER | THR | LEU | THR | ARG | TRP | ALA | ||||
| 2 | HIS | TYR | ASN | ALA | LEU | ILE | ARG | ALA | PHE |
Samples:
gH626-644: TFE, [U-99% 2H], 80 v/v; H2O 20 v/v; gH626-644 2 mM
gH626-644_conditions: pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | gH626-644 | isotropic | gH626-644_conditions |
| 2D 1H-1H NOESY | gH626-644 | isotropic | gH626-644_conditions |
| 2D DQF-COSY | gH626-644 | isotropic | gH626-644_conditions |
Software:
XEASY,CYANA, Bartels et al., Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution
NMR spectrometers:
- Varian INOVA 500 MHz