BMRB Entry 15824
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15824
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Title: Bank Vole Prion Protein (121-231) PubMed: 18773909
Deposition date: 2008-06-25 Original release date: 2008-09-12
Authors: Christen, Barbara; Hornemann, Simone; Wuthrich, Kurt
Citation: Christen, Barbara; Perez, Daniel; Hornemann, Simone; Wuthrich, Kurt. "NMR Structure of the Bank Vole Prion Protein at 20 degrees C Contains a Structured Loop of Residues 165-171" J. Mol. Biol. 383, 306-312 (2008).
Assembly members:
Prion_Protein, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Bank vole Taxonomy ID: 447135 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Clethrionomys glareolus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Prion_Protein: GSVVGGLGGYMLGSAMSRPM
IHFGNDWEDRYYRENMNRYP
NQVYYRPVDQYNNQNNFVHD
CVNITIKQHTVTTTTKGENF
TETDVKMMERVVEQMCVTQY
QKESQAYYEGRSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 364 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 414 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Prion Protein | 1 |
Entities:
Entity 1, Prion Protein 113 residues - Formula weight is not available
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | MET | ||||
| 3 | ILE | HIS | PHE | GLY | ASN | ASP | TRP | GLU | ASP | ARG | ||||
| 4 | TYR | TYR | ARG | GLU | ASN | MET | ASN | ARG | TYR | PRO | ||||
| 5 | ASN | GLN | VAL | TYR | TYR | ARG | PRO | VAL | ASP | GLN | ||||
| 6 | TYR | ASN | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | ||||
| 7 | CYS | VAL | ASN | ILE | THR | ILE | LYS | GLN | HIS | THR | ||||
| 8 | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | ||||
| 9 | THR | GLU | THR | ASP | VAL | LYS | MET | MET | GLU | ARG | ||||
| 10 | VAL | VAL | GLU | GLN | MET | CYS | VAL | THR | GLN | TYR | ||||
| 11 | GLN | LYS | GLU | SER | GLN | ALA | TYR | TYR | GLU | GLY | ||||
| 12 | ARG | SER | SER |
Samples:
15N: Prion Protein, [U-99% 15N], 1 mM; D2O 10%; H2O 90%; sodium acetate, [U-2H], 10 mM; H2O 90%; D2O, [U-100% 2H], 10%
13C_15N: Prion Protein, [U-99% 13C; U-99% 15N], 1 mM; D2O 10%; H2O 90%; sodium acetate, [U-2H], 10 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: ionic strength: 0.01 M; pH: 4.5; pressure: 1 atm; temperature: 293.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | 15N | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | 13C_15N | isotropic | sample_conditions_1 |
Software:
OPAL v1.2, Luginbuhl, Guntert, Billeter and Wuthrich - refinement
CARA v1.5.5, Keller, R. - data analysis
ATHNOS-CANDID v1.2, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking
DYANA v1.0.3, Guntert, Braun and Wuthrich - structure solution
Molmol v2K.2, Koradi, Billeter and Wuthrich - data analysis
xwinnmr, Bruker Biospin - collection
NMR spectrometers:
- Bruker DRX 750 MHz
- Bruker Avance 900 MHz
Related Database Links:
| BMRB | 15845 |
| PDB | |
| GB | AAL57231 AAL57232 ABR23642 ACJ06784 ACV85679 |
| REF | XP_005365625 XP_006984054 XP_007609107 |