BMRB Entry 16122
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16122
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Title: HCV NS4B(227-254) PubMed: 19357161
Deposition date: 2009-01-15 Original release date: 2009-04-17
Authors: Montserret, Roland; Penin, Francois
Citation: Gouttenoire, Jerome; Castet, Valerie; Montserret, Roland; Arora, Naveen; Raussens, Vincent; Ruysschaert, Jean-Marie; Diesis, Eric; Blum, Hubert; Penin, Francois; Moradpour, Darius. "Identification of a Novel Determinant for Membrane Association in Hepatitis C Virus Nonstructural Protein 4B" J. Virol 83, 6257-6268 (2009).
Assembly members:
HCV NS4B(227-254), polymer, 28 residues, 3111.629 Da.
Natural source: Common Name: Hepatitis C Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C Virus
Experimental source: Production method: chemical synthesis Host organism: none
Entity Sequences (FASTA):
HCV NS4B(227-254): SDAAARVTAILSSLTVTQLL
RRLHQWIS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 82 |
| 1H chemical shifts | 177 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HCV NS4B(227-254) | 1 |
Entities:
Entity 1, HCV NS4B(227-254) 28 residues - 3111.629 Da.
| 1 | SER | ASP | ALA | ALA | ALA | ARG | VAL | THR | ALA | ILE | ||||
| 2 | LEU | SER | SER | LEU | THR | VAL | THR | GLN | LEU | LEU | ||||
| 3 | ARG | ARG | LEU | HIS | GLN | TRP | ILE | SER |
Samples:
sample_1: entity 2 mM; H2O 50%; trifluoroethanol 50%
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - data analysis
NMR spectrometers:
- Bruker Avance 500 MHz