BMRB Entry 16601
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16601
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Title: kkgf PubMed: 22593157
Deposition date: 2009-11-09 Original release date: 2012-06-26
Authors: Montserret, Roland; Penin, Francois
Citation: Oehler, Verena; Filipe, Ana; Montserret, Roland; da Costa, Daniel; Brown, Gaie; Penin, Francois; McLauchlan, John. "Structural analysis of hepatitis C virus core-e1 signal Peptide and requirements for cleavage of the genotype 3a signal sequence by signal Peptide peptidase." J. Virol. 86, 7818-7828 (2012).
Assembly members:
HCV_Core-E1, polymer, 27 residues, 2881.567 Da.
Natural source: Common Name: Hepatitis C virus Taxonomy ID: 11103 Superkingdom: virus Kingdom: not available Genus/species: not available Hepatitis C
Experimental source: Production method: chemical synthesis Host organism: none
Entity Sequences (FASTA):
HCV_Core-E1: KKGFPFSIFLLALLSCITVP
VSAAQVK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 75 |
| 1H chemical shifts | 164 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HCV_Core-E1 | 1 |
Entities:
Entity 1, HCV_Core-E1 27 residues - 2881.567 Da.
| 1 | LYS | LYS | GLY | PHE | PRO | PHE | SER | ILE | PHE | LEU | ||||
| 2 | LEU | ALA | LEU | LEU | SER | CYS | ILE | THR | VAL | PRO | ||||
| 3 | VAL | SER | ALA | ALA | GLN | VAL | LYS |
Samples:
sample_1: kkgf 2 ± 0.1 mM; DSS 0.01 mM; H2O 50 %v/v; trifluoroethanol d2OH 50 %v/v
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - data collection
SPARKY, Goddard - chemical shift assignment
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
Molmol, Koradi, Billeter and Wuthrich - structures superposition
TALOS, Cornilescu, Delaglio and Bax - dihedral angle calculations
NMR spectrometers:
- Bruker Avance 500 MHz