BMRB Entry 16634
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16634
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Title: Backbone Dynamics and Global Effects of an Activity Enhancing Mutation in Minimized Mtu RecA Inteins PubMed: 20562025
Deposition date: 2009-12-15 Original release date: 2010-06-28
Authors: Du, Zhenming; Liu, Yangzhong; Ban, David; Lopez, Maria; Belfort, Marlene; Wang, Chunyu
Citation: Du, Zhenming; Liu, Yangzhong; Ban, David; Lopez, Maria; Belfort, Marlene; Wang, Chunyu. "Backbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins." J. Mol. Biol. 400, 755-767 (2010).
Assembly members:
Mtu_RecA_intein, polymer, 139 residues, Formula weight is not available
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Mtu_RecA_intein: CLAEGTRIFDPVTGTTHRIE
DVVGGRKPIHVVAAAKDGTL
HARPVVSWFDQGTRDVIGLR
IAGGAIVWATPDHKVLTEYG
WRAAGELRKGDRVAVRDVET
GELRYSVIREVLPTRRARTF
GLEVEELHTLVAEGVVVHN
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 132 |
| 1H chemical shifts | 132 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ddIhh-V67CM | 1 |
Entities:
Entity 1, ddIhh-V67CM 139 residues - Formula weight is not available
| 1 | CYS | LEU | ALA | GLU | GLY | THR | ARG | ILE | PHE | ASP | ||||
| 2 | PRO | VAL | THR | GLY | THR | THR | HIS | ARG | ILE | GLU | ||||
| 3 | ASP | VAL | VAL | GLY | GLY | ARG | LYS | PRO | ILE | HIS | ||||
| 4 | VAL | VAL | ALA | ALA | ALA | LYS | ASP | GLY | THR | LEU | ||||
| 5 | HIS | ALA | ARG | PRO | VAL | VAL | SER | TRP | PHE | ASP | ||||
| 6 | GLN | GLY | THR | ARG | ASP | VAL | ILE | GLY | LEU | ARG | ||||
| 7 | ILE | ALA | GLY | GLY | ALA | ILE | VAL | TRP | ALA | THR | ||||
| 8 | PRO | ASP | HIS | LYS | VAL | LEU | THR | GLU | TYR | GLY | ||||
| 9 | TRP | ARG | ALA | ALA | GLY | GLU | LEU | ARG | LYS | GLY | ||||
| 10 | ASP | ARG | VAL | ALA | VAL | ARG | ASP | VAL | GLU | THR | ||||
| 11 | GLY | GLU | LEU | ARG | TYR | SER | VAL | ILE | ARG | GLU | ||||
| 12 | VAL | LEU | PRO | THR | ARG | ARG | ALA | ARG | THR | PHE | ||||
| 13 | GLY | LEU | GLU | VAL | GLU | GLU | LEU | HIS | THR | LEU | ||||
| 14 | VAL | ALA | GLU | GLY | VAL | VAL | VAL | HIS | ASN |
Samples:
sample_1: Mtu RecA intein, [U-99% 13C; U-99% 15N], 0.15 – 0.3 mM; H2O 90%; D2O 10%; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
SPARKY, Goddard - chemical shift assignment
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 15560 17414 |
| PDB | |
| EMBL | CFR77613 CMM98736 |
| GB | EPZ65824 KAM73204 |
| REF | WP_049963343 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts