BMRB Entry 1704
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR1704
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Title: Two-Dimensional NMR Studies of Staphylococcal Nuclease: Evidence for Conformational Heterogeneity from Hydrogen-1, Carbon-13, and Nitrogen-15 Spin System Assignments of the Aromatic Amino Acids in the Nuclease H124L-Thymidine 3',5'-Bisphosp
Deposition date: 1995-07-31 Original release date: 1995-07-31
Authors: Wang, Jinfeng; Hinck, Andrew; Loh, Stewart; Markley, John
Citation: Wang, Jinfeng; Hinck, Andrew; Loh, Stewart; Markley, John. "Two-Dimensional NMR Studies of Staphylococcal Nuclease: Evidence for Conformational Heterogeneity from Hydrogen-1, Carbon-13, and Nitrogen-15 Spin System Assignments of the Aromatic Amino Acids in the Nuclease H124L-Thymidine 3',5'-Bisphosp" Biochemistry 29, 4242-4253 (1990).
Assembly members:
micrococcal nuclease, polymer, 143 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aereus
Experimental source: Production method: not available
Entity Sequences (FASTA):
micrococcal nuclease: ATSTKKLHKEPATLIKAIDG
DTVKLMYKGQPMTFRLLLVD
TPETKHPKKGVEKYGPEASA
FTKKMVENAKKIEVEFDKGQ
RTDKYGRGLAYIYADGKMVN
EALVRQGLAKVAYVYKPNNT
HEQLLRKSEAQAKKEKLNIW
SED
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 100 |
| 15N chemical shifts | 5 |
| 1H chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | micrococcal nuclease | 1 |
Entities:
Entity 1, micrococcal nuclease 143 residues - Formula weight is not available
| 1 | ALA | THR | SER | THR | LYS | LYS | LEU | HIS | LYS | GLU | ||||
| 2 | PRO | ALA | THR | LEU | ILE | LYS | ALA | ILE | ASP | GLY | ||||
| 3 | ASP | THR | VAL | LYS | LEU | MET | TYR | LYS | GLY | GLN | ||||
| 4 | PRO | MET | THR | PHE | ARG | LEU | LEU | LEU | VAL | ASP | ||||
| 5 | THR | PRO | GLU | THR | LYS | HIS | PRO | LYS | LYS | GLY | ||||
| 6 | VAL | GLU | LYS | TYR | GLY | PRO | GLU | ALA | SER | ALA | ||||
| 7 | PHE | THR | LYS | LYS | MET | VAL | GLU | ASN | ALA | LYS | ||||
| 8 | LYS | ILE | GLU | VAL | GLU | PHE | ASP | LYS | GLY | GLN | ||||
| 9 | ARG | THR | ASP | LYS | TYR | GLY | ARG | GLY | LEU | ALA | ||||
| 10 | TYR | ILE | TYR | ALA | ASP | GLY | LYS | MET | VAL | ASN | ||||
| 11 | GLU | ALA | LEU | VAL | ARG | GLN | GLY | LEU | ALA | LYS | ||||
| 12 | VAL | ALA | TYR | VAL | TYR | LYS | PRO | ASN | ASN | THR | ||||
| 13 | HIS | GLU | GLN | LEU | LEU | ARG | LYS | SER | GLU | ALA | ||||
| 14 | GLN | ALA | LYS | LYS | GLU | LYS | LEU | ASN | ILE | TRP | ||||
| 15 | SER | GLU | ASP |
Samples:
sample_one:
sample_condition_set_one: pH: 5.5 na; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_condition_set_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 136 1581 1582 16585 17718 18013 1874 1875 1876 1877 1878 18788 188 189 2784 2785 4010 4052 4053 |
| PDB | |
| DBJ | BAB41979 BAB56977 BAB94634 BAF67032 BAF77694 |
| EMBL | CAA24594 CAG39855 CAG42530 CAI80436 CAQ49298 |
| GB | AAC14660 AAW36415 ABD22328 ABD29945 ABE02272 |
| PRF | 1109959A 710414A |
| REF | WP_000141556 WP_000141557 WP_001548082 WP_001566557 WP_001574556 |
| SP | P00644 Q5HHM4 Q6GB41 Q6GIK1 Q7A6P2 |