BMRB Entry 17043
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17043
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Title: TMS2 domain of Dengue virus NS4A protein
Deposition date: 2010-07-06 Original release date: 2014-03-05
Authors: Montserret, Roland; Bartenschlager, Ralf; Penin, Francois
Citation: Montserret, Roland; Penin, Francois; Bartenschlager, Ralf. "TMS2 domain of Dengue virus NS4A protein" J. Biol. Chem. ., .-..
Assembly members:
TMS2, polymer, 34 residues, 3661.534 Da.
Natural source: Common Name: Dengue virus Taxonomy ID: 12637 Superkingdom: not available Kingdom: not available Genus/species: Flavivirus not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
TMS2: KKSGRGIGKMTLGMCCIITA
SILLWYAQIQGGKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 96 |
| 1H chemical shifts | 221 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TMS2 domain | 1 |
Entities:
Entity 1, TMS2 domain 34 residues - 3661.534 Da.
two solubilisation tag added to the sequence ; KK in N terminus and GGKK in C terminus
| 1 | LYS | LYS | SER | GLY | ARG | GLY | ILE | GLY | LYS | MET | ||||
| 2 | THR | LEU | GLY | MET | CYS | CYS | ILE | ILE | THR | ALA | ||||
| 3 | SER | ILE | LEU | LEU | TRP | TYR | ALA | GLN | ILE | GLN | ||||
| 4 | GLY | GLY | LYS | LYS |
Samples:
sample_1: entity 1 ± 0.1 mM; trifluoroethanol, [U-99% 2H], 50 ± 1 % v/v; H2O 50 ± 1 % v/v; DTT, [U-99% 2H], 20 ± 1 mM; DSS 0.2 ± 0.05 mM
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
X-PLOR NIH v2.24, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz