BMRB Entry 18287
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18287
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Title: Backbone and sidechain 1H chemical shifts for PAP248-286 (SEVI) in solution at pH 6 PubMed: 22360607
Deposition date: 2012-02-21 Original release date: 2012-03-22
Authors: Popovych, Nataliya; Brender, Jeffrey; Soong, Ronald; Vivekanandan, Subramanian; Hartman, Kevin; Basrur, Venkatesha; MacDonald, Peter; Ramamoorthy, Ayyalusamy
Citation: Popovych, Nataliya; Brender, Jeffrey; Soong, Ronald; Vivekanandan, Subramanian; Hartman, Kevin; Basrur, Venkatesha; Macdonald, Peter; Ramamoorthy, Ayyalusamy. "Site specific interaction of the polyphenol EGCG with the SEVI amyloid precursor peptide PAP(248-286)." J. Phys. Chem. B 116, 3650-3658 (2012).
Assembly members:
PAP248-286, polymer, 39 residues, Formula weight is not available
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
PAP248-286: GIHKQKEKSRLQGGVLVNEI
LNHMKRATQIPSYKKLIMY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 230 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PAP248-286 | 1 |
Entities:
Entity 1, PAP248-286 39 residues - Formula weight is not available
| 1 | GLY | ILE | HIS | LYS | GLN | LYS | GLU | LYS | SER | ARG | ||||
| 2 | LEU | GLN | GLY | GLY | VAL | LEU | VAL | ASN | GLU | ILE | ||||
| 3 | LEU | ASN | HIS | MET | LYS | ARG | ALA | THR | GLN | ILE | ||||
| 4 | PRO | SER | TYR | LYS | LYS | LEU | ILE | MET | TYR |
Samples:
sample_1: PAP248-286 0.3 mM; sodium phosphate 50 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 6; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - data analysis
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 900 MHz