BMRB Entry 18636
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18636
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Title: 1H, 13C and 15N resonance assignment of the soluble form of the Lipid-modified Azurin from Neisseria gonorrhoeae PubMed: 23070845
Deposition date: 2012-08-03 Original release date: 2012-10-18
Authors: Pauleta, Sofia; Matzapetakis, Manolis; Nobrega, Claudia
Citation: Nobrega, Claudia; Matzapetakis, Manolis; Pauleta, Sofia. "1H, 13C and 15N resonance assignment of the soluble form of the Lipid-modified Azurin from Neisseria gonorrhoeae." Biomol. NMR Assignments 7, 311-314 (2013).
Assembly members:
Laz, polymer, 130 residues, 13630.2539 Da.
Natural source: Common Name: b-proteobacteria Taxonomy ID: 485 Superkingdom: Bacteria Kingdom: not available Genus/species: Neisseria gonorrhoeae
Experimental source: Production method: recombinant technology Host organism: undefined
Entity Sequences (FASTA):
Laz: ATAGNCAATVESNDNMQFNT
KDIQVSKACKEFTITLKHTG
TQPKASMGHNLVIAKAEDMD
GVFKDGVGAADTDYVKPDDA
RVVAHTKLIGGGEESSLTLD
PAKLADGDYKFACTFPGHGA
LMNGKVTLVD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 544 |
| 15N chemical shifts | 139 |
| 1H chemical shifts | 859 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Laz | 1 |
Entities:
Entity 1, Laz 130 residues - 13630.2539 Da.
| 1 | ALA | THR | ALA | GLY | ASN | CYS | ALA | ALA | THR | VAL | |
| 2 | GLU | SER | ASN | ASP | ASN | MET | GLN | PHE | ASN | THR | |
| 3 | LYS | ASP | ILE | GLN | VAL | SER | LYS | ALA | CYS | LYS | |
| 4 | GLU | PHE | THR | ILE | THR | LEU | LYS | HIS | THR | GLY | |
| 5 | THR | GLN | PRO | LYS | ALA | SER | MET | GLY | HIS | ASN | |
| 6 | LEU | VAL | ILE | ALA | LYS | ALA | GLU | ASP | MET | ASP | |
| 7 | GLY | VAL | PHE | LYS | ASP | GLY | VAL | GLY | ALA | ALA | |
| 8 | ASP | THR | ASP | TYR | VAL | LYS | PRO | ASP | ASP | ALA | |
| 9 | ARG | VAL | VAL | ALA | HIS | THR | LYS | LEU | ILE | GLY | |
| 10 | GLY | GLY | GLU | GLU | SER | SER | LEU | THR | LEU | ASP | |
| 11 | PRO | ALA | LYS | LEU | ALA | ASP | GLY | ASP | TYR | LYS | |
| 12 | PHE | ALA | CYS | THR | PHE | PRO | GLY | HIS | GLY | ALA | |
| 13 | LEU | MET | ASN | GLY | LYS | VAL | THR | LEU | VAL | ASP |
Samples:
13C15NLaz: Laz, [U-98% 13C; U-98% 15N], 1.5 mM; Phosphate 20.0 mM; Azide 1.0 mM; Ascorbate 5.0 mM; H2O 90%; D2O 10%
15NLaz: Laz, [U-98% 15N], 1.0 mM; Phosphate 20 mM; Azide 1 mM; Ascorbate 5 mM; H2O 90%; D2O 10%
1: ionic strength: 0.035 M; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | 13C15NLaz | isotropic | 1 |
| b_HNCO | 13C15NLaz | isotropic | 1 |
| b_HNcaCo | 13C15NLaz | isotropic | 1 |
| b_HNcoCACB | 13C15NLaz | isotropic | 1 |
| b_HNCACB | 13C15NLaz | isotropic | 1 |
| 13C_HSQC | 13C15NLaz | isotropic | 1 |
| hCCH_TOCSY | 13C15NLaz | isotropic | 1 |
| HNHA | 15NLaz | isotropic | 1 |
| 13C HSQC NOESY | 13C15NLaz | isotropic | 1 |
| 15N HSQC NOESY | 13C15NLaz | isotropic | 1 |
| J2 HSQC | 13C15NLaz | isotropic | 1 |
Software:
CARA v1.8, Keller and Wuthrich - Assignment
ANALYSIS v2.2, CCPN - data analysis
TOPSPIN v2.1, Bruker Biospin - Processing
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts