BMRB Entry 19050
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PDB ID: 2m5e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19050
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Title: Structure of the C-domain of Calcium-saturated Calmodulin bound to the IQ motif of NaV1.2 PubMed: 28343066
Deposition date: 2013-02-21 Original release date: 2014-07-21
Authors: Fowler, C.; Feldkamp, Michael; Yu, Liping; Shea, Madeline
Citation: Hovey, Liam; Fowler, C; Mahling, Ryan; Lin, Zesen; Miller, Mark; Marx, Dagan; Yoder, Jesse; Kim, Elaine; Tefft, Kristin; Waite, Brett; Feldkamp, Michael; Yu, Liping; Shea, Madeline. "Calcium triggers reversal of calmodulin on nested anti-parallel sites in the IQ motif of the neuronal voltage-dependent sodium channel NaV1.2." Biophys. Chem. 224, 1-19 (2017).
Assembly members:
C-domain_of_Calmodulin_(residues_76-148), polymer, 73 residues, 8416.369 Da.
IQ_motif_peptide_of_NaV1-2_(residues_1901-1927), polymer, 27 residues, 3370.125 Da.
CALCIUM ION, non-polymer, 40.078 Da.
Natural source: Common Name: Ciliates Taxonomy ID: 5888 Superkingdom: Eukaryota Kingdom: not available Genus/species: Paramecium tetraurelia
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
C-domain_of_Calmodulin_(residues_76-148): MKEQDSEEELIEAFKVFDRD
GNGLISAAELRHVMTNLGEK
LTDDEVDEMIREADIDGDGH
INYEEFVRMMVSK
IQ_motif_peptide_of_NaV1-2_(residues_1901-1927): KRKQEEVSAIVIQRAYRRYL
LKQKVKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 314 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 606 |
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