BMRB Entry 19398
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19398
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Title: Solution structure of the forkhead domain of Brugia malayi DAF-16a
Deposition date: 2013-07-30 Original release date: 2013-09-10
Authors: Veldkamp, C.; Peterson, F.; Casper, S.; Schoeller, S.
Citation: Veldkamp, C.; Peterson, F.; Casper, S.; Schoeller, S.. "Solution structure of the forkhead domain of Brugia malayi DAF-16a" To be published ., .-..
Assembly members:
Bm-DAF-16a, polymer, 101 residues, 11673.083 Da.
Natural source: Common Name: nematodes Taxonomy ID: 6279 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Brugia malayi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Bm-DAF-16a: NPWGAESYSDLIAKALKSTF
DGRMRLNEIYNWFASNVPYF
GNRTSQEQSAGWKNSIRHNL
SLHSRFMRIQNEGAGKSSWW
VINPDAKPGRNPRRQRSATL
E
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 423 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 687 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Forkhead DNA binding domain of Brugia malayi DAF-16a | 1 |
Entities:
Entity 1, Forkhead DNA binding domain of Brugia malayi DAF-16a 101 residues - 11673.083 Da.
| 1 | ASN | PRO | TRP | GLY | ALA | GLU | SER | TYR | SER | ASP | ||||
| 2 | LEU | ILE | ALA | LYS | ALA | LEU | LYS | SER | THR | PHE | ||||
| 3 | ASP | GLY | ARG | MET | ARG | LEU | ASN | GLU | ILE | TYR | ||||
| 4 | ASN | TRP | PHE | ALA | SER | ASN | VAL | PRO | TYR | PHE | ||||
| 5 | GLY | ASN | ARG | THR | SER | GLN | GLU | GLN | SER | ALA | ||||
| 6 | GLY | TRP | LYS | ASN | SER | ILE | ARG | HIS | ASN | LEU | ||||
| 7 | SER | LEU | HIS | SER | ARG | PHE | MET | ARG | ILE | GLN | ||||
| 8 | ASN | GLU | GLY | ALA | GLY | LYS | SER | SER | TRP | TRP | ||||
| 9 | VAL | ILE | ASN | PRO | ASP | ALA | LYS | PRO | GLY | ARG | ||||
| 10 | ASN | PRO | ARG | ARG | GLN | ARG | SER | ALA | THR | LEU | ||||
| 11 | GLU |
Samples:
sample: Forkhead box DNA binding domain of Brugia malayi DAF-16a, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 90%; D20 10%; sodium phosphate 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_15N-separated_NOESY | sample | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY | sample | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY (AROMATIC) | sample | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement
TOPSPIN v2.1, Bruker - collection
NMRPipe v2007, Delagio,F. et al. - processing
XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis
GARANT v2.1, C. Bartels - data analysis
CARA v1.8.4, Keller, R. - data analysis
CYANA v3.0, Guntert, P. - structural calculation
NMR spectrometers:
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts