BMRB Entry 36008

Assembly:

Entities:

Entity 1, entity_1 111 residues - 12809.266 Da.

Samples:

sample_1: DTT 2 mM; EDTA 0.1 mM; SUMO1 K39Ac, [U-100% 13C; U-100% 15N], 1 mM; potassium chloride 100 mM; potassium phosphate 10 mM; sodium azide 0.001%; H2O 90%; D2O, [U-2H], 10%

sample_2: DTT 2 mM; EDTA 0.1 mM; SUMO1 K39Ac, [U-100% 15N], 1 mM; potassium chloride 100 mM; potassium phosphate 10 mM; sodium azide 0.001%; H2O 90%; D2O, [U-2H], 10%

sample_3: DTT 2 mM; EDTA 0.1 mM; Pf1 phage 10 mg/mL; SUMO1 K39Ac, [U-100% 15N], 1 mM; potassium chloride 100 mM; potassium phosphate 10 mM; sodium azide 0.001%; H2O 90%; D2O, [U-2H], 10%

sample_4: DTT 2 mM; EDTA 0.1 mM; SUMO1 K39Ac 1 mM; potassium chloride 100 mM; potassium phosphate 10 mM; sodium azide 0.001%; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 290 K

Experiments:

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

SPARKY v3.113, Goddard - data analysis

TOPSPIN v3.2, Bruker Biospin - processing

X-PLOR NIH v2.34, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

• Bruker Avance 500 MHz
• Bruker AvanceIII 600 MHz
• Bruker Avance 800 MHz
• Bruker AvanceIII 850 MHz