BMRB Entry 4035
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4035
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Title: Solution Structure of the DNA-Binding Domain of a Human Papillomavirus E2 Protein: Evidence for Flexible DNA-Binding Regions
Deposition date: 1997-06-16 Original release date: 2005-04-21
Authors: Liang, Heng; Petros, Andrew; Meadows, Robert; Yoon, Ho; Egan, David; Walter, Karl; Holzman, Thomas; Robins, Terry; Fesik, Stephen
Citation: Liang, Heng; Petros, Andrew; Meadows, Robert; Yoon, Ho; Egan, David; Walter, Karl; Holzman, Thomas; Robins, Terry; Fesik, Stephen. "Solution Structure of the DNA-Binding Domain of a Human Papillomavirus E2 Protein: Evidence for Flexible DNA-Binding Regions" Biochemistry 35, 2095-2103 (1996).
Assembly members:
Regulatory protein E2, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: human Papillomavirus Taxonomy ID: 10566 Superkingdom: not available Kingdom: not available Genus/species: Papillomavirus human papillomavirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Regulatory protein E2: MATTPIIHLKGDANILKCLR
YRLSKYKQLYEQVSSTWHWT
CTDGKHKNAIVTLTYISTSQ
RDDFLNTVKIPNTVSVSTGY
MTI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 296 |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 599 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E2-HPV | 1 |
Entities:
Entity 1, E2-HPV 83 residues - Formula weight is not available
| 1 | MET | ALA | THR | THR | PRO | ILE | ILE | HIS | LEU | LYS | ||||
| 2 | GLY | ASP | ALA | ASN | ILE | LEU | LYS | CYS | LEU | ARG | ||||
| 3 | TYR | ARG | LEU | SER | LYS | TYR | LYS | GLN | LEU | TYR | ||||
| 4 | GLU | GLN | VAL | SER | SER | THR | TRP | HIS | TRP | THR | ||||
| 5 | CYS | THR | ASP | GLY | LYS | HIS | LYS | ASN | ALA | ILE | ||||
| 6 | VAL | THR | LEU | THR | TYR | ILE | SER | THR | SER | GLN | ||||
| 7 | ARG | ASP | ASP | PHE | LEU | ASN | THR | VAL | LYS | ILE | ||||
| 8 | PRO | ASN | THR | VAL | SER | VAL | SER | THR | GLY | TYR | ||||
| 9 | MET | THR | ILE |
Samples:
sample_one: Regulatory protein E2, [U-15N;U-13C], 1.7 mM; D2O 10%; H2O 90%; sodium_phosphate 20 mM; dithiothreitol 5 mM
sample_two: Regulatory protein E2, [U-15N;U-13C], 1.7 mM; D2O 100%; sodium_phosphate 20 mM; dithiothreitol 5 mM
sample_conditions_one: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker AMX600 600 MHz
- Bruker DMX500 500 MHz
- Bruker AMX500 500 MHz
Related Database Links:
| PDB | |
| EMBL | CRH59757 CRH70730 |
| GB | AAA46953 AEI60921 AEI60929 AEI60937 AEI60945 |
| REF | WP_057257005 |
| SP | P17383 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts