BMRB Entry 4271
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4271
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Title: Sequence Specific 1H, 13C and 15N Assignments of a Calcium Binding Protein from Entamoeba Histolytica
Deposition date: 1998-11-30 Original release date: 2000-05-25
Authors: Govil, Girjesh; Chary, Kandala; Sahu, Sarata; Chauhan, Sanjay; Bhattacharya, A.; Atreya, H.
Citation: Govil, Girjesh; Chary, Kandala; Sahu, Sarata; Chauhan, Sanjay; Bhattacharya, A.; Atreya, H.. "Sequence Specific 1H, 13C and 15N Assignments of a Calcium Binding Protein from Entamoeba Histolytica" J. Biomol. NMR 14, 93-94 (1999).
Assembly members:
Entamoeba Histolytica CaBP, polymer, 134 residues, 14939 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Entamoeba Histolytica Taxonomy ID: 5759 Superkingdom: Eukaryota Kingdom: not available Genus/species: Entamoeba histolytica
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Entamoeba Histolytica CaBP: MAEALFKEIDVNGDGAVSYE
EVKAFVSKKRAIKNEQLLQL
IFKSIDADGNGEIDQNEFAK
FYGSIQGQDLSDDKIGLKVL
YKLMDVDGDGKLTKEEVTSF
FKKHGIEKVAEQVMKADANG
DGYITLEEFLEFSL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 496 |
| 13C chemical shifts | 380 |
| 15N chemical shifts | 131 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Eh CaBP | 1 |
| 2 | Calcium Ion | 2 |
Entities:
Entity 1, Eh CaBP 134 residues - 14939 Da.
| 1 | MET | ALA | GLU | ALA | LEU | PHE | LYS | GLU | ILE | ASP | ||||
| 2 | VAL | ASN | GLY | ASP | GLY | ALA | VAL | SER | TYR | GLU | ||||
| 3 | GLU | VAL | LYS | ALA | PHE | VAL | SER | LYS | LYS | ARG | ||||
| 4 | ALA | ILE | LYS | ASN | GLU | GLN | LEU | LEU | GLN | LEU | ||||
| 5 | ILE | PHE | LYS | SER | ILE | ASP | ALA | ASP | GLY | ASN | ||||
| 6 | GLY | GLU | ILE | ASP | GLN | ASN | GLU | PHE | ALA | LYS | ||||
| 7 | PHE | TYR | GLY | SER | ILE | GLN | GLY | GLN | ASP | LEU | ||||
| 8 | SER | ASP | ASP | LYS | ILE | GLY | LEU | LYS | VAL | LEU | ||||
| 9 | TYR | LYS | LEU | MET | ASP | VAL | ASP | GLY | ASP | GLY | ||||
| 10 | LYS | LEU | THR | LYS | GLU | GLU | VAL | THR | SER | PHE | ||||
| 11 | PHE | LYS | LYS | HIS | GLY | ILE | GLU | LYS | VAL | ALA | ||||
| 12 | GLU | GLN | VAL | MET | LYS | ALA | ASP | ALA | ASN | GLY | ||||
| 13 | ASP | GLY | TYR | ILE | THR | LEU | GLU | GLU | PHE | LEU | ||||
| 14 | GLU | PHE | SER | LEU |
Entity 2, Calcium Ion - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: Entamoeba Histolytica CaBP, [U-98% 13C; U-98% 15N], 3 – 4 mM
sample_2: Entamoeba Histolytica CaBP, [U-98% 15N], 3 – 4 mM
cabp1: pH: 6.0; temperature: 308 K; ionic strength: 0.05 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | not available | not available | cabp1 |
| 15N-TOCSY-HSQC | not available | not available | cabp1 |
| 15N-NOESY-HSQC | not available | not available | cabp1 |
| HCA | not available | not available | cabp1 |
| H(CO)CA | not available | not available | cabp1 |
| HCO | not available | not available | cabp1 |
| CBCANH | not available | not available | cabp1 |
| CBCA(CO)NH | not available | not available | cabp1 |
Software:
Felix v95.0 - Some C programmes were written with peak lists from triple resonance spectra as input to make the assignments semi-automated.
NMR spectrometers:
- Varian Unity plus 600 MHz
Related Database Links:
| BMRB | 19197 |
| PDB | |
| DBJ | BAN39246 |
| GB | AAA29089 EAL48959 EKE39141 EMD43507 EMH75928 |
| REF | XP_008858522 XP_654345 |
| SP | P38505 |
Download simulated HSQC data in one of the following formats:
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