BMRB Entry 4346
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4346
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Title: 1H, 13C, and 15N Chemical Shift Assignments for the Regulatory mRNA of the Yeast Ribosomal Protein L30
Deposition date: 1999-05-07 Original release date: 2000-03-08
Authors: Mao, Hongyuan; White, Susan; Williamson, james
Citation: Mao, Hongyuan; White, Susan; Williamson, james. "A Novel Loop-loop Recognition Motif in the Yeast Ribosomal Protein L30 Autoregulatory RNA Complex" Nat. Struct. Biol. 6, 1139-1147 (1999).
Assembly members:
L30 MRNA, polymer, 33 residues, Formula weight is not available
Natural source: Common Name: BAKER Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota FUNGI
Experimental source: Production method: enzymatic semisynthesis
Entity Sequences (FASTA):
L30 MRNA: GGACCGGAGUGUCGCAAGAC
GCAGAGAUGGUCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 193 |
| 15N chemical shifts | 24 |
| 1H chemical shifts | 255 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | L30RNA | 1 |
Entities:
Entity 1, L30RNA 33 residues - Formula weight is not available
| 1 | G | G | A | C | C | G | G | A | G | U | ||||
| 2 | G | U | C | G | C | A | A | G | A | C | ||||
| 3 | G | C | A | G | A | G | A | U | G | G | ||||
| 4 | U | C | C |
Samples:
SAMPLE_1: L30 MRNA, N/A, 1.8 mM
SAMPLE_2: L30 MRNA, [U-99% 13C; U-99% 15N], 0.9 mM
SAMPLE_3: L30 MRNA, [U-95% 13C; U-99% 15N]-G, 0.5 mM
Ex-cond_1: ionic strength: 0.1 M; pH: 6.0; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
NMRPipe -
NMR spectrometers:
- VARIAN INOVA 600 MHz
- FBML FBML 500 MHz