BMRB Entry 4389
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4389
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Title: Backbone NMR Assignment and Secondary Structure of Ribosome Recycling Factor (RRF) from Pseudomonas aeruginosa
Deposition date: 1999-09-04 Original release date: 2000-12-15
Authors: Kashimori, Hiromasa; Yoshida, Takuya; Kijima, Hiroyuki; Shimahara, Hideto; Uchiyama, Susumu; Ishino, Tetsuya; Shuda, Masahiro; Nakano, Hiroaki; Shibata, Yasutaka; Saihara, Yukari; Ohkubo, Tadayasu; Yoshida, Toshio; Kaji, Akira; Kobayashi, Yuji
Citation: Kashimori, Hiromasa; Yoshida, Takuya; Kijima, Hiroyuki; Shimahara, Hideto; Uchiyama, Susumu; Ishino, Tetsuya; Shuda, Masahiro; Nakano, Hiroaki; Shibata, Yasutaka; Saihara, Yukari; Ohkubo, Tadayasu; Yoshida, Toshio; Kaji, Akira; Kobayashi, Yuji. "Backbone NMR Assignment and Secondary Structure of Ribosome Recycling Factor (RRF) from Pseudomonas aeruginosa" J. Biomol. NMR 15, 341-342 (1999).
Assembly members:
Ribosome Recycling Factor, polymer, 185 residues, 20485 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Ribosome Recycling Factor: MINEIKKEAQERMGKTLEAL
GHAFAKIRTGRAHPSILDSV
MVSYYGADTPLRQVANVTVE
DSRTLALAVFDKSMIQAVEK
AIMTSDLGLNPATAGTTIRV
PMPALTEETRKGYTKQARAE
AEQARVSVRNIRRDALAQLK
DLQKEKEISEDEERRAGDDV
QKLTDKFIGEIEKALEAKEA
DLMAV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 357 |
| 15N chemical shifts | 173 |
| 1H chemical shifts | 346 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RRF | 1 |
Entities:
Entity 1, RRF 185 residues - 20485 Da.
| 1 | MET | ILE | ASN | GLU | ILE | LYS | LYS | GLU | ALA | GLN | ||||
| 2 | GLU | ARG | MET | GLY | LYS | THR | LEU | GLU | ALA | LEU | ||||
| 3 | GLY | HIS | ALA | PHE | ALA | LYS | ILE | ARG | THR | GLY | ||||
| 4 | ARG | ALA | HIS | PRO | SER | ILE | LEU | ASP | SER | VAL | ||||
| 5 | MET | VAL | SER | TYR | TYR | GLY | ALA | ASP | THR | PRO | ||||
| 6 | LEU | ARG | GLN | VAL | ALA | ASN | VAL | THR | VAL | GLU | ||||
| 7 | ASP | SER | ARG | THR | LEU | ALA | LEU | ALA | VAL | PHE | ||||
| 8 | ASP | LYS | SER | MET | ILE | GLN | ALA | VAL | GLU | LYS | ||||
| 9 | ALA | ILE | MET | THR | SER | ASP | LEU | GLY | LEU | ASN | ||||
| 10 | PRO | ALA | THR | ALA | GLY | THR | THR | ILE | ARG | VAL | ||||
| 11 | PRO | MET | PRO | ALA | LEU | THR | GLU | GLU | THR | ARG | ||||
| 12 | LYS | GLY | TYR | THR | LYS | GLN | ALA | ARG | ALA | GLU | ||||
| 13 | ALA | GLU | GLN | ALA | ARG | VAL | SER | VAL | ARG | ASN | ||||
| 14 | ILE | ARG | ARG | ASP | ALA | LEU | ALA | GLN | LEU | LYS | ||||
| 15 | ASP | LEU | GLN | LYS | GLU | LYS | GLU | ILE | SER | GLU | ||||
| 16 | ASP | GLU | GLU | ARG | ARG | ALA | GLY | ASP | ASP | VAL | ||||
| 17 | GLN | LYS | LEU | THR | ASP | LYS | PHE | ILE | GLY | GLU | ||||
| 18 | ILE | GLU | LYS | ALA | LEU | GLU | ALA | LYS | GLU | ALA | ||||
| 19 | ASP | LEU | MET | ALA | VAL |
Samples:
sample_1: Ribosome Recycling Factor, [U-99% 2H; U-99% 15N], 1.5 mM
sample_2: Ribosome Recycling Factor, [U-99% 2H; U-99% 13C; U-99% 15N], 1.5 mM
sample_conditions_1: ionic strength: 0.8 M; pH: 5.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | sample_conditions_1 |
| 3D HNCA | not available | not available | sample_conditions_1 |
| 3D HN(CO)CA | not available | not available | sample_conditions_1 |
| 3D HNCO | not available | not available | sample_conditions_1 |
| 3D HA(CA)NH | not available | not available | sample_conditions_1 |
| 3D HA(CACO)NH | not available | not available | sample_conditions_1 |
| 4D 1H-15N-15N-1H NOESY | not available | not available | sample_conditions_1 |
Software:
PIPP v4.2.5 - peak assignments
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| DBJ | BAA32345 BAK91607 BAP21725 BAP49359 BAQ38186 |
| EMBL | CAW26110 CCQ88442 CDH69697 CDH75776 CDI92077 |
| GB | AAG07041 ABJ12886 ABR84529 AEO73780 AFM63508 |
| REF | NP_252343 WP_003092390 WP_003109339 WP_012074694 WP_023097294 |
| SP | A6V1D5 B7V7G0 O82853 Q02RC5 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts