BMRB Entry 4528
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4528
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Title: 1H, 13C, and 15N Chemical Shift Assignments for the N-terminal receiver domain of NtrC (phosphorylated)
Deposition date: 1999-11-05 Original release date: 2000-06-16
Authors: Kern, D.; Volkman, B.; Luginbuhl, P.; Nohaile, M.; Kustu, S.; Wemmer, D.
Citation: Kern, D.; Volkman, B.; Luginbuhl, P.; Nohaile, M.; Kustu, S.; Wemmer, D.. "Structure of a Transiently Phosphorylated "Switch" in Bacterial Signal Transduction" Nature 402, 894-898 (1999).
Assembly members:
NITROGEN REGULATION PROTEIN, polymer, 124 residues, Formula weight is not available
PO4, non-polymer, 94.971 Da.
Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 602 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella typhimurium
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NITROGEN REGULATION PROTEIN: MQRGIVWVVDDDSSIRWVLE
RALAGAGLTCTTFENGNEVL
AALASKTPDVLLSDIRMPGM
DGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFD
YLPKPFDIDEAVALVERAIS
HYQE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 750 |
| 13C chemical shifts | 363 |
| 15N chemical shifts | 113 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | P-NtrCr | 1 |
| 2 | PO4 | 2 |
Entities:
Entity 1, P-NtrCr 124 residues - Formula weight is not available
| 1 | MET | GLN | ARG | GLY | ILE | VAL | TRP | VAL | VAL | ASP | ||||
| 2 | ASP | ASP | SER | SER | ILE | ARG | TRP | VAL | LEU | GLU | ||||
| 3 | ARG | ALA | LEU | ALA | GLY | ALA | GLY | LEU | THR | CYS | ||||
| 4 | THR | THR | PHE | GLU | ASN | GLY | ASN | GLU | VAL | LEU | ||||
| 5 | ALA | ALA | LEU | ALA | SER | LYS | THR | PRO | ASP | VAL | ||||
| 6 | LEU | LEU | SER | ASP | ILE | ARG | MET | PRO | GLY | MET | ||||
| 7 | ASP | GLY | LEU | ALA | LEU | LEU | LYS | GLN | ILE | LYS | ||||
| 8 | GLN | ARG | HIS | PRO | MET | LEU | PRO | VAL | ILE | ILE | ||||
| 9 | MET | THR | ALA | HIS | SER | ASP | LEU | ASP | ALA | ALA | ||||
| 10 | VAL | SER | ALA | TYR | GLN | GLN | GLY | ALA | PHE | ASP | ||||
| 11 | TYR | LEU | PRO | LYS | PRO | PHE | ASP | ILE | ASP | GLU | ||||
| 12 | ALA | VAL | ALA | LEU | VAL | GLU | ARG | ALA | ILE | SER | ||||
| 13 | HIS | TYR | GLN | GLU |
Entity 2, PO4 - O4 P - 94.971 Da.
| 1 | PO4 |
Samples:
sample_1: NITROGEN REGULATION PROTEIN, [U-15N], 0.3 mM; Na phosphate 200 mM; MgCl2 50 mM; Carbamoylphosphate 200 mM
sample_2: NITROGEN REGULATION PROTEIN, [U-15N; U-13C], 0.3 mM; Na phosphate 200 mM; MgCl2 50 mM; Carbamoylphosphate 200 mM
sample_cond_1: pH: 6.75; temperature: 298 K; ionic strength: 375 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-SEPARATED_NOESY | not available | not available | sample_cond_1 |
| 3D 13C-SEPARATED_NOESY | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
Software:
DYANA v1.5 - STRUCTURE SOLUTION, DATA ANALYSIS
FELIX v95.0 - PROCESSING
XWINNMR v1.5 - COLLECTION
XEASY v1.3.13 - DATA ANALYSIS
NMR spectrometers:
- Bruker DMX 750 MHz
Related Database Links:
| BMRB | 25124 25125 4527 |
| PDB | |
| DBJ | BAB38213 BAE77441 BAG79675 BAI27885 BAI33008 |
| EMBL | CAA28808 CAA59425 CAD03095 CAP78325 CAQ34219 |
| GB | AAB03002 AAC76865 AAG59057 AAL22844 AAN45373 |
| PIR | AC0950 E86074 |
| REF | NP_312817 NP_418304 NP_458044 NP_462885 NP_709666 |
| SP | P0AFB8 P0AFB9 P41789 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts