BMRB Entry 5378
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5378
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Title: NMR Structure of a Variant Human Prion Protein with Two Disulfide Bridges PubMed: 12547204
Deposition date: 2002-05-17 Original release date: 2003-02-20
Authors: Zahn, Ralph; Guntert, Peter; von Schroetter, Christine; Wuthrich, Kurt
Citation: Zahn, Ralph; Guntert, Peter; von Schroetter, Christine; Wuthrich, Kurt. "NMR Structure of a Variant Human Prion Protein with Two Disulfide Bridges" J. Mol. Biol. 326, 225-234 (2003).
Assembly members:
human prion protein 121-230, polymer, 112 residues, 13092 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
human prion protein 121-230: GSVVGGLGGYMLGSAMSRPI
IHFGSDYEDRYYRENMHRYP
NQVYYRPCDEYSNQNNFVHD
CVNITIKQHTVTTTTKGENF
TETDVKMMERVVEQMCITQY
ERCSQAYYQRGS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 770 |
| 13C chemical shifts | 321 |
| 15N chemical shifts | 130 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hprp(121-230) subunit 1 | 1 |
Entities:
Entity 1, hprp(121-230) subunit 1 112 residues - 13092 Da.
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | ILE | ||||
| 3 | ILE | HIS | PHE | GLY | SER | ASP | TYR | GLU | ASP | ARG | ||||
| 4 | TYR | TYR | ARG | GLU | ASN | MET | HIS | ARG | TYR | PRO | ||||
| 5 | ASN | GLN | VAL | TYR | TYR | ARG | PRO | CYS | ASP | GLU | ||||
| 6 | TYR | SER | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | ||||
| 7 | CYS | VAL | ASN | ILE | THR | ILE | LYS | GLN | HIS | THR | ||||
| 8 | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | ||||
| 9 | THR | GLU | THR | ASP | VAL | LYS | MET | MET | GLU | ARG | ||||
| 10 | VAL | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | ||||
| 11 | GLU | ARG | CYS | SER | GLN | ALA | TYR | TYR | GLN | ARG | ||||
| 12 | GLY | SER |
Samples:
sample_1: human prion protein 121-230, [U-15N; U-13C], 0.7 mM
sample_2: human prion protein 121-230 0.7 mM
Ex-cond_1: pH: 4.5 na; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H-15N/13C NOESY | not available | not available | not available |
| 1H-1H-15N TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HSQC | not available | not available | not available |
Software:
PROSA -
XEASY -
DYANA-CANDID -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 750 MHz
Related Database Links:
| BMRB | 15676 17756 17780 18426 4379 4402 4434 4620 4736 5713 |
| PDB | |
| DBJ | BAA00011 BAG32276 BAG32278 BAG32279 BAG52189 |
| EMBL | CAA58442 |
| GB | AAA19664 AAA60182 AAA68633 AAB59442 AAB59443 |
| REF | NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902 |
| SP | P04156 P40252 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts