BMRB Entry 6046
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6046
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Title: 1H, 13C and 15N resonance assignments for domain III of the West Nile Virus envelope protein PubMed: 15213461
Deposition date: 2003-12-12 Original release date: 2004-07-06
Authors: Volk, David; Kallick, Deborah; Holbrook, Michael; Beasley, David; Barrett, Alan; Gorenstein, David
Citation: Volk, David; Kallick, Deborah; Holbrook, Michael; Beasley, David; Barrett, Alan; Gorenstein, David. "Letter to the Editor: 1H, 13C and 15N resonance assignments for domain III of the West Nile Virus envelope protein" J. Biomol. NMR 29, 445-446 (2004).
Assembly members:
West Nile Virus envelope protein domain III, polymer, 115 residues, 12185 Da.
Natural source: Common Name: West Nile Virus I strain 385-99 Taxonomy ID: 11082 Superkingdom: Viruses Kingdom: not available Genus/species: Flavivirus West Nile Virus I
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
West Nile Virus envelope protein domain III: ISEFQLKGTTYGVCSKAFKF
LGTPADTGHGTVVLELQYTG
TDGPCKVPISSVASLNDLTP
VGRLVTVNPFVSVATANAKV
LIELEPPFGDSYIVVGRGEQ
QINHHWHKSGSSIGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 444 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 743 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | wnd3 subunit 1 | 1 |
Entities:
Entity 1, wnd3 subunit 1 115 residues - 12185 Da.
| 1 | ILE | SER | GLU | PHE | GLN | LEU | LYS | GLY | THR | THR | ||||
| 2 | TYR | GLY | VAL | CYS | SER | LYS | ALA | PHE | LYS | PHE | ||||
| 3 | LEU | GLY | THR | PRO | ALA | ASP | THR | GLY | HIS | GLY | ||||
| 4 | THR | VAL | VAL | LEU | GLU | LEU | GLN | TYR | THR | GLY | ||||
| 5 | THR | ASP | GLY | PRO | CYS | LYS | VAL | PRO | ILE | SER | ||||
| 6 | SER | VAL | ALA | SER | LEU | ASN | ASP | LEU | THR | PRO | ||||
| 7 | VAL | GLY | ARG | LEU | VAL | THR | VAL | ASN | PRO | PHE | ||||
| 8 | VAL | SER | VAL | ALA | THR | ALA | ASN | ALA | LYS | VAL | ||||
| 9 | LEU | ILE | GLU | LEU | GLU | PRO | PRO | PHE | GLY | ASP | ||||
| 10 | SER | TYR | ILE | VAL | VAL | GLY | ARG | GLY | GLU | GLN | ||||
| 11 | GLN | ILE | ASN | HIS | HIS | TRP | HIS | LYS | SER | GLY | ||||
| 12 | SER | SER | ILE | GLY | LYS |
Samples:
sample_1: West Nile Virus envelope protein domain III, [U-95% 13C; U-95% 15N], 0.7 mM; K2HPO4 50 mM; NaCl 100 mM; NaN3 10 mM; EDTA 0.1 mM
Ex-cond_1: ionic strength: 0.26 M; pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| H(CC)CONH-TOCSY | not available | not available | not available |
| (H)CC(CO)NH-TOCSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 15N-edited NOESY-HSQC | not available | not available | not available |
| 13C-edited NOESY-HSQC | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian UnityPlus 750 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
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