BMRB Entry 6130
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6130
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Title: Solution structure and backbone dynamics of the Cu(I) and apo-forms of the second metal-binding domain of the Menkes protein ATP7A PubMed: 15035611
Deposition date: 2004-03-03 Original release date: 2004-05-15
Authors: Banci, L.; Bertini, I.; Del Conte, R.; D'Onofrio, M.; Rosato, A.
Citation: Banci, L.; Bertini, I.; Del Conte, R.; D'Onofrio, M.; Rosato, A.. "Solution structure and backbone dynamics of the Cu(I) and apo forms of the second metal-binding domain of the Menkes protein ATP7A" Biochemistry 43, 3396-3403 (2004).
Assembly members:
ATPase, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ATPase: GEVVLKMKVEGMTCHSCTST
IEGKIGKLQGVQRIKVSLDN
QEATIVYQPHLISVEEMKKQ
IEAMGFPAFVKKIEGR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 320 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 524 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Copper-transporting ATPase 1 | 1 |
Entities:
Entity 1, Copper-transporting ATPase 1 76 residues - Formula weight is not available
| 1 | GLY | GLU | VAL | VAL | LEU | LYS | MET | LYS | VAL | GLU | ||||
| 2 | GLY | MET | THR | CYS | HIS | SER | CYS | THR | SER | THR | ||||
| 3 | ILE | GLU | GLY | LYS | ILE | GLY | LYS | LEU | GLN | GLY | ||||
| 4 | VAL | GLN | ARG | ILE | LYS | VAL | SER | LEU | ASP | ASN | ||||
| 5 | GLN | GLU | ALA | THR | ILE | VAL | TYR | GLN | PRO | HIS | ||||
| 6 | LEU | ILE | SER | VAL | GLU | GLU | MET | LYS | LYS | GLN | ||||
| 7 | ILE | GLU | ALA | MET | GLY | PHE | PRO | ALA | PHE | VAL | ||||
| 8 | LYS | LYS | ILE | GLU | GLY | ARG |
Samples:
sample_1: ATPase 1 mM; phosphate 100 mM; H2O 90%; D2O 10%
sample_2: ATPase, [U-15N], 1 mM; phosphate 100 mM; H2O 90%; D2O 10%
sample_3: ATPase, [U-13C; U-15N], 1 mM; phosphate 100 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| [1H-1H]-NOESY | not available | not available | sample_cond_1 |
| 13C-NOESY-HSQC | not available | not available | sample_cond_1 |
| 15N-NOESY-HSQC | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
Software:
DYANA v1.5 - refinement, structure solution
NMR spectrometers:
- unknown unknown 0 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts