BMRB Entry 6383
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6383
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Title: NMR solution structure of the recombinant elk and mouse/elk variant prion proteins
Deposition date: 2004-11-10 Original release date: 2005-01-14
Authors: Gossert, Alvar; Bonjour, Sophie; Lysek, Dominikus
Citation: Gossert, Alvar; Bonjour, Sophie; Lysek, Dominikus; Fiorito, Francesco; Wuthrich, Kurt. "Prion protein NMR structures of the elk and of mouse/elk hybrids" Proc. Natl. Acad. Sci. U.S.A. 102, 646-650 (2005).
Assembly members:
elk prion protein, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: American elk Taxonomy ID: 9864 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: cervus Cervus elaphus
Experimental source: Production method: recombinant technology Host organism: Eschericia coli
Entity Sequences (FASTA):
elk prion protein: GSVVGGLGGYMLGSAMSRPL
IHFGNDYEDRYYRENMYRYP
NQVYYRPVDQYNNQNTFVHD
CVNITVKQHTVTTTTKGENF
TETDIKMMERVVEQMCITQY
QRESEAYYQRGAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 325 |
| 15N chemical shifts | 132 |
| 1H chemical shifts | 800 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | eprp monomer | 1 |
Entities:
Entity 1, eprp monomer 113 residues - Formula weight is not available
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | LEU | ||||
| 3 | ILE | HIS | PHE | GLY | ASN | ASP | TYR | GLU | ASP | ARG | ||||
| 4 | TYR | TYR | ARG | GLU | ASN | MET | TYR | ARG | TYR | PRO | ||||
| 5 | ASN | GLN | VAL | TYR | TYR | ARG | PRO | VAL | ASP | GLN | ||||
| 6 | TYR | ASN | ASN | GLN | ASN | THR | PHE | VAL | HIS | ASP | ||||
| 7 | CYS | VAL | ASN | ILE | THR | VAL | LYS | GLN | HIS | THR | ||||
| 8 | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | ||||
| 9 | THR | GLU | THR | ASP | ILE | LYS | MET | MET | GLU | ARG | ||||
| 10 | VAL | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | ||||
| 11 | GLN | ARG | GLU | SER | GLU | ALA | TYR | TYR | GLN | ARG | ||||
| 12 | GLY | ALA | SER |
Samples:
sample_1: elk prion protein, [U-13C; U-15N], 1 mM
conditions_1: pH: 4.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-edited NOESY | sample_1 | not available | conditions_1 |
| 13C-edited NOESY | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 750 MHz
Related Database Links:
| PDB | |
| EMBL | CAA70902 |
| GB | AAA68941 AAB61573 AAB94788 AAC12860 AAC12861 |
| SP | P47852 P67986 P67987 Q5UJG3 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts