BMRB Entry 6403
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6403
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Title: Ovine Prion Protein Variant R168
Deposition date: 2004-11-29 Original release date: 2004-12-23
Authors: Christen, Barbara; Wuethrich, Kurt
Citation: Lysek, Dominikus; Schorn, Christian; Nivon, Lucas; Esteve-Moya, Vicent; Christen, Barbara; Calzolai, Luigi; von Schoetter, Christine; Fiorito, Francesco; Herrmann, Torsten; Guntert, Peter; Wuthrich, Kurt. "Prion protein NMR structures of cat, dog, pig, and sheep" Proc. Natl. Acad. Sci. U.S.A. ., .-..
Assembly members:
sheep prion protein, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Sheep Taxonomy ID: 9940 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ovis aries
Experimental source: Production method: recombinant technology Host organism: Eschericia coli
Entity Sequences (FASTA):
sheep prion protein: GSVVGGLGGYMLGSAMSRPL
IHFGNDYEDRYYRENMYRYP
NQVYYRPVDRYSNQNNFVHD
CVNITVKQHTVTTTTKGENF
TETDIKIMERVVEQMCITQY
QRESQAYYQRGAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 353 |
| 15N chemical shifts | 131 |
| 1H chemical shifts | 808 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ovine Prion Protein Variant R168 | 1 |
Entities:
Entity 1, Ovine Prion Protein Variant R168 113 residues - Formula weight is not available
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | LEU | ||||
| 3 | ILE | HIS | PHE | GLY | ASN | ASP | TYR | GLU | ASP | ARG | ||||
| 4 | TYR | TYR | ARG | GLU | ASN | MET | TYR | ARG | TYR | PRO | ||||
| 5 | ASN | GLN | VAL | TYR | TYR | ARG | PRO | VAL | ASP | ARG | ||||
| 6 | TYR | SER | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | ||||
| 7 | CYS | VAL | ASN | ILE | THR | VAL | LYS | GLN | HIS | THR | ||||
| 8 | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | ||||
| 9 | THR | GLU | THR | ASP | ILE | LYS | ILE | MET | GLU | ARG | ||||
| 10 | VAL | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | ||||
| 11 | GLN | ARG | GLU | SER | GLN | ALA | TYR | TYR | GLN | ARG | ||||
| 12 | GLY | ALA | SER |
Samples:
sample_1: sheep prion protein, [U-15N], 0.75 mM; sodium acetate, [U-2H], 10 mM; H2O 90%; D2O 10%
sample_2: sheep prion protein, [U-13C; U-15N], 0.5 mM; sodium acetate, [U-2H], 10 mM; D2O 100%
conditions_1: pH: 4.50; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | conditions_1 |
| 3D 13C-separated NOESY (ali) | not available | not available | conditions_1 |
| 3D 13C-separated NOESY (arom) | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 750 MHz
- Bruker AVANCE 900 MHz
Related Database Links:
| BMRB | 6381 |
| PDB | |
| DBJ | BAA07376 BAB69955 BAB69956 BAB69957 BAH03441 |
| EMBL | CAA04235 CAA04236 CAA04272 CAA04273 CAA04274 |
| GB | AAB70468 AAB97765 AAC78726 AAD14409 AAD19980 |
| PRF | 1921132A |
| REF | NP_001009481 |
| SP | P23907 P52113 Q5UJG1 Q5XVM4 Q68G95 |
Download simulated HSQC data in one of the following formats:
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