BMRB Entry 6824
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6824
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Title: 1H, 13C, and 15N Chemical Shift Assignments for ORF 7a coded X4 protein of SARS Coronavirus PubMed: 16328780
Deposition date: 2005-09-13 Original release date: 2007-01-29
Authors: Stangler, Thomas; Stoldt, Matthias; Willbold, Dieter
Citation: Hanel, Karen; Stangler, Thomas; Stoldt, Matthias; Willbold, Dieter. "Solution structure of the X4 protein coded by the SARS related coronavirus reveals an immunoglobulin like fold and suggests a binding activity to integrin I domains." J. Biomed. Sci. 13, 281-293 (2005).
Assembly members:
SARS CoV 7a protein, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: SARS Coronavirus Taxonomy ID: 227859 Superkingdom: Viruses Kingdom: Not applicable Genus/species: Coronavirus SARS Coronavirus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SARS CoV 7a protein: GPELYHYQECVRGTTVLLKE
PCPSGTYEGNSPFHPLADNK
FALTCTSTHFAFACADGTRH
TYQLRARSVSPKLFIRQEEV
QQELYSR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 350 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 524 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SARS CoV 7a protein | 1 |
Entities:
Entity 1, SARS CoV 7a protein 87 residues - Formula weight is not available
| 1 | GLY | PRO | GLU | LEU | TYR | HIS | TYR | GLN | GLU | CYS | ||||
| 2 | VAL | ARG | GLY | THR | THR | VAL | LEU | LEU | LYS | GLU | ||||
| 3 | PRO | CYS | PRO | SER | GLY | THR | TYR | GLU | GLY | ASN | ||||
| 4 | SER | PRO | PHE | HIS | PRO | LEU | ALA | ASP | ASN | LYS | ||||
| 5 | PHE | ALA | LEU | THR | CYS | THR | SER | THR | HIS | PHE | ||||
| 6 | ALA | PHE | ALA | CYS | ALA | ASP | GLY | THR | ARG | HIS | ||||
| 7 | THR | TYR | GLN | LEU | ARG | ALA | ARG | SER | VAL | SER | ||||
| 8 | PRO | LYS | LEU | PHE | ILE | ARG | GLN | GLU | GLU | VAL | ||||
| 9 | GLN | GLN | GLU | LEU | TYR | SER | ARG |
Samples:
sample_1: SARS CoV 7a protein, [U-95% 13C; U-98% 15N], 0.4 mM; Sodium Acetate, [U-2H], 1 mM
conditions_1: ionic strength: 5 mM; pH: 5; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| H(CCO)NH-TOCSY | sample_1 | not available | conditions_1 |
| C(CO)NH-TOCSY | sample_1 | not available | conditions_1 |
| HCCH-COSY | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| 1H-13C-HSQC-NOESY | sample_1 | not available | conditions_1 |
Software:
NMRPipe - Processing
CARA v1.1 - data evaluation
NMR spectrometers:
- Varian Unity INOVA 600 MHz
- Varian Unity Inova 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC81354 BAC81368 BAC81382 BAC81396 BAC81410 |
| GB | AAP13449 AAP13573 AAP30036 AAP33703 AAP37023 |
| REF | NP_828857 |
| SP | P59635 Q3I5J0 Q3LZX7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts