BMRB Entry 7063
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7063
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Title: Solution NMR structure of the putative cytoplasmic protein ygaC from Salmonella typhimurium. Northeast Structural Genomics target StR72.
Deposition date: 2006-04-10 Original release date: 2007-02-01
Authors: Aramini, J.; Swapna, G.; Ramelot, T.; Ho, C.; Cunningham, K.; Ma, L.-C.; Shetty, K.; Xiao, R.; Acton, T.; Kennedy, M.; Montelione, G.
Citation: Aramini, J.; Swapna, G.; Ramelot, T.; Ho, C.; Cunningham, K.; Ma, L.-C.; Shetty, K.; Xiao, R.; Acton, T.; Kennedy, M.; Montelione, G.. "Solution NMR structure of the putative cytoplasmic protein ygaC from Salmonella typhimurium. Northeast Structural Genomics target StR72." . ., .-..
Assembly members:
putative cytoplasmic protein, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 602 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella typhimurium
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
putative cytoplasmic protein: MYLRPDEVARVLEKAGFTVD
VVTNKTYGYRRGENYVYVNR
EARMGRTALIIHPRLKDRSS
SLADPASDIKTCDHYQNFPL
YLGGETHEHYGIPHGFSSRI
ALERYLNGLFGDEKTDLEHH
HHHH
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 538 |
| 15N chemical shifts | 126 |
| 1H chemical shifts | 831 |
| coupling constants | 65 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | putative cytoplasmic protein | 1 |
Entities:
Entity 1, putative cytoplasmic protein 124 residues - Formula weight is not available
| 1 | MET | TYR | LEU | ARG | PRO | ASP | GLU | VAL | ALA | ARG | ||||
| 2 | VAL | LEU | GLU | LYS | ALA | GLY | PHE | THR | VAL | ASP | ||||
| 3 | VAL | VAL | THR | ASN | LYS | THR | TYR | GLY | TYR | ARG | ||||
| 4 | ARG | GLY | GLU | ASN | TYR | VAL | TYR | VAL | ASN | ARG | ||||
| 5 | GLU | ALA | ARG | MET | GLY | ARG | THR | ALA | LEU | ILE | ||||
| 6 | ILE | HIS | PRO | ARG | LEU | LYS | ASP | ARG | SER | SER | ||||
| 7 | SER | LEU | ALA | ASP | PRO | ALA | SER | ASP | ILE | LYS | ||||
| 8 | THR | CYS | ASP | HIS | TYR | GLN | ASN | PHE | PRO | LEU | ||||
| 9 | TYR | LEU | GLY | GLY | GLU | THR | HIS | GLU | HIS | TYR | ||||
| 10 | GLY | ILE | PRO | HIS | GLY | PHE | SER | SER | ARG | ILE | ||||
| 11 | ALA | LEU | GLU | ARG | TYR | LEU | ASN | GLY | LEU | PHE | ||||
| 12 | GLY | ASP | GLU | LYS | THR | ASP | LEU | GLU | HIS | HIS | ||||
| 13 | HIS | HIS | HIS | HIS |
Samples:
sample_1: putative cytoplasmic protein, [U-13C; U-15N], 1.12 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%
sample_2: putative cytoplasmic protein, [U-13C; U-15N], 1.12 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 100%; H2O 95%
sample_3: putative cytoplasmic protein, [U-5% 13C; U-15N], 1.07 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%
sample_cond_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| 4D 13C-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| high resolution CH-HSQC (stereospecific assignment of Val/Leu methyls) | not available | not available | not available |
| backbone TR expts | not available | not available | not available |
| 3D HCCH-COSY | not available | not available | not available |
| 3D TOCSYs | not available | not available | not available |
| aromatic expts | not available | not available | not available |
Software:
xwinnmr v3.5pl6 - collection
VNMR v6.1C - collection
SPARKY v3.110 - data analysis
AutoAssign v1.17.0 - data analysis
AutoStruct v2.1.1 - refinement
X-PLOR NIH v2.11.2 - refinement
CNS v1.1 - refinement
PDBStat v4.01 - data analysis
PSVS v1.2 - data analysis
NMR spectrometers:
- Varian INOVA 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAJ37798 BAP08706 |
| EMBL | CAD05911 CAR34225 CAR38517 CBG25775 CBW18883 |
| GB | AAL21686 AAO70265 AAX66640 ABX68827 ACF63119 |
| PIR | AH0840 |
| REF | NP_457200 NP_461727 NP_806405 WP_000281302 WP_000281303 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts