BMRB Entry 11506

Assembly:

Entities:

Entity 1, entity 403 residues - 46071.996 Da.

Samples:

sample_1: entity, [U-13C; U-15N; U-2H; 1H ILE, LEU, VAL, ALA METHYL; 1H MET METHYL,GAMMA; 1H PHE EPSILON] VRK1, 0.4 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; glutamic acid 50 mM; arginine 50 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_2: entity, [U-13C; U-15N; U-2H; 1H ILE, LEU, VAL METHYL; 1H PHE, TYR EPSILON] VRK1, 0.4 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; glutamic acid 50 mM; arginine 50 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_3: entity, [U-15N; U-2H; 13C,1H ILE, LEU, VAL METHYL; 13C,1H PHE DELTA/EPSILON/ZETA] VRK1, 0.4 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; glutamic acid 50 mM; arginine 50 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_4: entity, SAIL-VRK1, 0.4 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; glutamic acid 50 mM; arginine 50 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

AZARA, Boucher - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

• Bruker Avance 900 MHz