BMRB Entry 15225
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15225
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Title: 1H, 13C, and 15N resonance assignments of domain 2 of non-structural protein 5A (NS5A) of hepatitis C virus PubMed: 17880107
Deposition date: 2007-04-26 Original release date: 2007-10-29
Authors: Liang, Yu; Ye, Hong; Kang, Cong Bao; Yoon, Ho Sup
Citation: Liang, Yu; Ye, Hong; Kang, Cong Bao; Yoon, Ho Sup. "Domain 2 of Non-structural Protein 5A (NS5A) of Hepatitis C Virus is Natively Unfolded" Biochemistry 46, 11550-11558 (2007).
Assembly members:
Domain_2_of_Non-structural_Protein_5A_(NS5AD2), polymer, 102 residues, Formula weight is not available
Natural source: Common Name: hepatitis c virus Taxonomy ID: 11103 Superkingdom: not available Kingdom: Viruses Genus/species: not available Hepacivirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Domain_2_of_Non-structural_Protein_5A_(NS5AD2): KATCTANHDSPDAELIEANL
LWRQEMGGNITRVESENKVV
ILDSFDPLVAEEDEREVSVP
AEILRKSRRFARALPVWARP
DYNPPLVETWKKPDYEHHHH
HH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 385 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 636 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Domain 2 of Non-structural Protein 5A (NS5AD2) | 1 |
Entities:
Entity 1, Domain 2 of Non-structural Protein 5A (NS5AD2) 102 residues - Formula weight is not available
| 1 | LYS | ALA | THR | CYS | THR | ALA | ASN | HIS | ASP | SER | ||||
| 2 | PRO | ASP | ALA | GLU | LEU | ILE | GLU | ALA | ASN | LEU | ||||
| 3 | LEU | TRP | ARG | GLN | GLU | MET | GLY | GLY | ASN | ILE | ||||
| 4 | THR | ARG | VAL | GLU | SER | GLU | ASN | LYS | VAL | VAL | ||||
| 5 | ILE | LEU | ASP | SER | PHE | ASP | PRO | LEU | VAL | ALA | ||||
| 6 | GLU | GLU | ASP | GLU | ARG | GLU | VAL | SER | VAL | PRO | ||||
| 7 | ALA | GLU | ILE | LEU | ARG | LYS | SER | ARG | ARG | PHE | ||||
| 8 | ALA | ARG | ALA | LEU | PRO | VAL | TRP | ALA | ARG | PRO | ||||
| 9 | ASP | TYR | ASN | PRO | PRO | LEU | VAL | GLU | THR | TRP | ||||
| 10 | LYS | LYS | PRO | ASP | TYR | GLU | HIS | HIS | HIS | HIS | ||||
| 11 | HIS | HIS |
Samples:
sample_1: Domain 2 of Non-structural Protein 5A (NS5AD2), [U-15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.01%
sample_2: Domain 2 of Non-structural Protein 5A (NS5AD2), [U-13C; U-15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.01%
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v1.3, Bruker Biospin - collection, processing
FELIX v2004, Accelrys Software Inc. - data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| EMBL | CAJ20207 CAJ20220 |
| GB | AAA45534 AAA45676 AAA45677 AAB66324 AAB67036 |
| REF | NP_671491 NP_751927 |
| SP | P26664 P27958 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts