BMRB Entry 4774
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4774
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Title: Three-dimensional Structure of the Histidine-containing Phosphocarrier Protein (Hpr) from Enterococcus faecalis in Solution PubMed: 11168402
Deposition date: 2000-07-03 Original release date: 2001-05-03
Authors: Maurer, Till; Doker, Rolf; Gorler, Adrian; Hengstenberg, Wolfgang; Kalbitzer, Hans
Citation: Maurer, Till; Doker, Rolf; Gorler, Adrian; Hengstenberg, Wolfgang; Kalbitzer, Hans. "Three-dimensional Structure of the Histidine-containing Phosphocarrier Protein (Hpr) from Enterococcus faecalis in Solution" Eur. J. Biochem. 268, 635-644 (2001).
Assembly members:
HISTIDINE CONTAINING PHOSPHCARRIER PROTEIN, polymer, 89 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1351 Superkingdom: Eubacteria Kingdom: Firmicutes Genus/species: Enterococcus faecalis
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
HISTIDINE CONTAINING PHOSPHCARRIER PROTEIN: MEKKEFHIVAETGIHARPAT
LLVQTASKFNSDINLEYKGK
SVNLKSIMGVMSLGVGQGSD
VTITVDGADEAEGMAAIVET
LQKEGLAEQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 609 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HPr | 1 |
Entities:
Entity 1, HPr 89 residues - Formula weight is not available
| 1 | MET | GLU | LYS | LYS | GLU | PHE | HIS | ILE | VAL | ALA | ||||
| 2 | GLU | THR | GLY | ILE | HIS | ALA | ARG | PRO | ALA | THR | ||||
| 3 | LEU | LEU | VAL | GLN | THR | ALA | SER | LYS | PHE | ASN | ||||
| 4 | SER | ASP | ILE | ASN | LEU | GLU | TYR | LYS | GLY | LYS | ||||
| 5 | SER | VAL | ASN | LEU | LYS | SER | ILE | MET | GLY | VAL | ||||
| 6 | MET | SER | LEU | GLY | VAL | GLY | GLN | GLY | SER | ASP | ||||
| 7 | VAL | THR | ILE | THR | VAL | ASP | GLY | ALA | ASP | GLU | ||||
| 8 | ALA | GLU | GLY | MET | ALA | ALA | ILE | VAL | GLU | THR | ||||
| 9 | LEU | GLN | LYS | GLU | GLY | LEU | ALA | GLU | GLN |
Samples:
sample_1: HISTIDINE CONTAINING PHOSPHCARRIER PROTEIN 5.0 mM
sample_cond_1: pH: 7.4; temperature: 298 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | not available | sample_cond_1 |
| COSY | sample_1 | not available | sample_cond_1 |
| TOCSY | sample_1 | not available | sample_cond_1 |
| NOESY-TOCSY | sample_1 | not available | sample_cond_1 |
Software:
DYANA v1.5 - STRUCTURE CALCULATION, REFINEMENT
XWINNMR - Data processing
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DMX 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAA79533 CBL32713 CCO71634 |
| GB | AAO80530 ADX79343 AEA93134 AFO43418 AHI39812 |
| REF | NP_814460 WP_002355551 WP_002358685 WP_002388527 WP_010776971 |
| SP | P07515 |