BMRB Entry 5804

Name: Three-disulfide variant of hen lysozyme: C76A/C94A
Published: 2004-09-14

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5804

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Three-disulfide variant of hen lysozyme: C76A/C94A PubMed: 15157100

Deposition date: 2003-05-19 Original release date: 2004-09-14

Authors: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi

Citation: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins." Biochemistry 43, 6663-6669 (2004).

Assembly members:
three-disulfide variant of hen lysozyme, polymer, 130 residues, 14374 Da.

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
three-disulfide variant of hen lysozyme: MKVFGRCELAAAMKRHGLDN YRGYSLGNWVCAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWCNDGRTPGSRNLANIP CSALLSSDITASVNAAKKIV SDGNGMNAWVAWRNRCKGTD VQAWIRGCRL

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	782

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	CYS	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	CYS	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	ALA	ASN	ILE	PRO
9	CYS	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	ALA	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	CYS	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

Name	Sample	Sample state	Sample conditions
DQF-COSY	not available	not available	not available
NOESY	not available	not available	not available
HOHAHA	not available	not available	not available
15N 3D-NOESY-HSQC	not available	not available	not available
15N 3D-TOCSY-HSQC	not available	not available	not available
1H-15N HSQC	not available	not available	not available
15N 3D-HSQC-NOESY-HSQC	not available	not available	not available

Biological Magnetic Resonance Data Bank

BMRB Entry 5804

Title: Three-disulfide variant of hen lysozyme: C76A/C94A PubMed: 15157100

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	CYS	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	CYS	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	ALA	ASN	ILE	PRO
9	CYS	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	ALA	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	CYS	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	CYS	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	CYS	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	ALA	ASN	ILE	PRO
9	CYS	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	ALA	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	CYS	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	CYS	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	CYS	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	ALA	ASN	ILE	PRO
9	CYS	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	ALA	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	CYS	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU