BMRB Entry 6415
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6415
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Three-disulfide variant of hen lysozyme: C30A/C115A PubMed: 15157100
Deposition date: 2004-12-07 Original release date: 2005-04-04
Authors: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi
Citation: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR Characterization of Three-Disulfide Variants of Lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A -A Marginally Stable State in Folded Proteins" Biochemistry 43, 6663-6669 (2004).
Assembly members:
hen egg white lysozyme variant, polymer, 130 residues, 14374 Da.
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
hen egg white lysozyme variant: MKVFGRCELAAAMKRHGLDN
YRGYSLGNWVAAAKFESNFN
TQATNRNTDGSTDYGILQIN
SRWWCNDGRTPGSRNLCNIP
CSALLSSDITASVNCAKKIV
SDGNGMNAWVAWRNRAKGTD
VQAWIRGCRL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 752 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hen lysozyme | 1 |
Entities:
Entity 1, hen lysozyme 130 residues - 14374 Da.
| 1 | MET | LYS | VAL | PHE | GLY | ARG | CYS | GLU | LEU | ALA | |
| 2 | ALA | ALA | MET | LYS | ARG | HIS | GLY | LEU | ASP | ASN | |
| 3 | TYR | ARG | GLY | TYR | SER | LEU | GLY | ASN | TRP | VAL | |
| 4 | ALA | ALA | ALA | LYS | PHE | GLU | SER | ASN | PHE | ASN | |
| 5 | THR | GLN | ALA | THR | ASN | ARG | ASN | THR | ASP | GLY | |
| 6 | SER | THR | ASP | TYR | GLY | ILE | LEU | GLN | ILE | ASN | |
| 7 | SER | ARG | TRP | TRP | CYS | ASN | ASP | GLY | ARG | THR | |
| 8 | PRO | GLY | SER | ARG | ASN | LEU | CYS | ASN | ILE | PRO | |
| 9 | CYS | SER | ALA | LEU | LEU | SER | SER | ASP | ILE | THR | |
| 10 | ALA | SER | VAL | ASN | CYS | ALA | LYS | LYS | ILE | VAL | |
| 11 | SER | ASP | GLY | ASN | GLY | MET | ASN | ALA | TRP | VAL | |
| 12 | ALA | TRP | ARG | ASN | ARG | ALA | LYS | GLY | THR | ASP | |
| 13 | VAL | GLN | ALA | TRP | ILE | ARG | GLY | CYS | ARG | LEU |
Samples:
sample_1: hen egg white lysozyme variant, [U-15N], 0.2 – 1.0 mM
EX-cond_1: pH: 3.8 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample_1 | not available | EX-cond_1 |
| NOESY | sample_1 | not available | EX-cond_1 |
| HOHAHA | sample_1 | not available | EX-cond_1 |
| 15N 3D-NOESY-HSQC | sample_1 | not available | EX-cond_1 |
| 15N 3D-TOCSY-HSQC | sample_1 | not available | EX-cond_1 |
| 1H-15N HSQC | sample_1 | not available | EX-cond_1 |
| 15N 3D-HSQC-NOESY-HSQC | sample_1 | not available | EX-cond_1 |
Software:
XWINNMR v2.6 -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| GenBank | AAL69327 AAA48943 |
| SWISS-PROT | P00698 |
| PDB | |
| 1 | . . . . . . . . . . . . . . . . |
| . | . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
| Hew | Lysozyme: |