BMRB Entry 6433
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6433
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Title: Sequence specific 1HN, 13C and 15N resonance assignments of a novel calcium binding protein from Entamoeba histolytica PubMed: 15929013
Deposition date: 2004-12-16 Original release date: 2005-07-26
Authors: Bansal-Mutalik, Ritu; Mustafi, Sourajit; Bhattacharya, Alok; Chary, V. R.
Citation: Bansal-Mutalik, Ritu; Mustafi, Sourajit; Bhattacharya, Alok; Chary, Kandala. "Letter to the Editor: Sequence specific 1HN, 13C and 15N resonance assignments of a novel calcium-binding protein from Entamoeba histolytica." J. Biomol. NMR 31, 379-380 (2005).
Assembly members:
Entamoeba histolytica calcium binding protein, polymer, 134 residues, 14944 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Entamoeba histolytica calcium binding protein: MAEALFKQLDANGDGSVSYE
EVKAFVSSKRPIKNEQLLQL
IFKAIDIDGNGEIDLAEFTK
FAAAVKEQDLSDEKVGLKIL
YKLMDADGDGKLTKEEVTTF
FKKFGYEKVVDQIMKADANG
DGYITLEEFLAFNL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 373 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 577 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EhCaBP2 | 1 |
| 2 | CALCIUM (II) ION | 2 |
Entities:
Entity 1, EhCaBP2 134 residues - 14944 Da.
| 1 | MET | ALA | GLU | ALA | LEU | PHE | LYS | GLN | LEU | ASP | ||||
| 2 | ALA | ASN | GLY | ASP | GLY | SER | VAL | SER | TYR | GLU | ||||
| 3 | GLU | VAL | LYS | ALA | PHE | VAL | SER | SER | LYS | ARG | ||||
| 4 | PRO | ILE | LYS | ASN | GLU | GLN | LEU | LEU | GLN | LEU | ||||
| 5 | ILE | PHE | LYS | ALA | ILE | ASP | ILE | ASP | GLY | ASN | ||||
| 6 | GLY | GLU | ILE | ASP | LEU | ALA | GLU | PHE | THR | LYS | ||||
| 7 | PHE | ALA | ALA | ALA | VAL | LYS | GLU | GLN | ASP | LEU | ||||
| 8 | SER | ASP | GLU | LYS | VAL | GLY | LEU | LYS | ILE | LEU | ||||
| 9 | TYR | LYS | LEU | MET | ASP | ALA | ASP | GLY | ASP | GLY | ||||
| 10 | LYS | LEU | THR | LYS | GLU | GLU | VAL | THR | THR | PHE | ||||
| 11 | PHE | LYS | LYS | PHE | GLY | TYR | GLU | LYS | VAL | VAL | ||||
| 12 | ASP | GLN | ILE | MET | LYS | ALA | ASP | ALA | ASN | GLY | ||||
| 13 | ASP | GLY | TYR | ILE | THR | LEU | GLU | GLU | PHE | LEU | ||||
| 14 | ALA | PHE | ASN | LEU |
Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: Entamoeba histolytica calcium binding protein, [U-13C; U-15N], 1.5 mM
cond_1: pH: 4.2; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| N15 HSQC | sample_1 | not available | cond_1 |
| CBCANH | sample_1 | not available | cond_1 |
| CBCA(CO)NH | sample_1 | not available | cond_1 |
| HNCA | sample_1 | not available | cond_1 |
| HN(CA)CO | sample_1 | not available | cond_1 |
| HNCO | sample_1 | not available | cond_1 |
Software:
Felix - Data processing
NMR spectrometers:
- Varian . 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA22013 |
| GB | AAR97975 EAL51694 EDR27146 EKE36904 EMD43571 |
| REF | XP_001736602 XP_008860769 XP_657080 |
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