BMRB Entry 5528

Name: Solution structure of the complementary RNA promoter of influenza a virus
Published: 2003-07-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5528

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of the complementary RNA promoter of influenza a virus PubMed: 12771209

Deposition date: 2002-09-13 Original release date: 2003-07-07

Authors: Park, C.-J.; Bae, S.-H.; Varani, G.; Lee, M.-K.; Choi, B.-S.

Citation: Park, C.-J.; Bae, S.-H.; Lee, M.-K.; Varani, G.; Choi, B.-S.. "Solution Structure of the Influenza A Virus cRNA Promoter: Implications for Differential Recognition of Viral Promoter Structures by RNA-dependent RNA Polymerase" Nucleic Acids Res. 31, 2824-2832 (2003).

Assembly members:
complementary RNA promoter of influenza virus, polymer, 25 residues, Formula weight is not available

Natural source: Common Name: influenza A virus Taxonomy ID: 11320 Superkingdom: not available Kingdom: not available Genus/species: influenzavirus A not available

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
complementary RNA promoter of influenza virus: GGAAGCAGGCUUCGGCCUUG UUUCC

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
31P chemical shifts	13

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	complementary RNA promoter	1

Entities:

Entity 1, complementary RNA promoter 25 residues - Formula weight is not available

1

G

A

G

C

A

G

C

2

U

C

G

C

U

G

3

U

C

Samples:

sample_1: complementary RNA promoter of influenza virus 1 mM; phosphate buffer 20 mM; EDTA 0.1 mM; H2O 90%; D2O 10%

sample_2: complementary RNA promoter of influenza virus, [U-15N; U-13C], 1 mM; phosphate buffer 20 mM; EDTA 0.1 mM; D2O 100%

sample_cond_1: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K

sample_cond_2: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 280 K

sample_cond_3: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 290 K

sample_cond_4: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
DQF-COSY	not available	not available	not available
HETCOR	not available	not available	not available
3D 13C NOESY-HSQC	not available	not available	not available
3D HCCH-COSY	not available	not available	not available
3D HCCH-COSY-TOCSY	not available	not available	not available

Software:

FELIX v95 - processing

VNMR v6.1c - collection

CNS v1.0 - refinement, structure solution

NMR spectrometers:

Varian INOVA 600 MHz